@article { , title = {Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity}, abstract = {Blood supply is essential for osteogenesis, yet its relationship to load-related increases in bone mass is poorly defined. Herein, we aim to investigate the link between load-induced osteogenesis and the blood supply (bone perfusion and vascular porosity) using an established osteogenic non-invasive model of axial loading. Accordingly, 12N mechanical loads were applied to the right tibiae of six male C57BL6 mice at 10-12 weeks of age, 3 times/week for two weeks. Skeletal perfusion was measured acutely (post-loading) and chronically in loaded and contralateral, non-loaded hindlimbs by Laser Doppler imaging. Vascular and lacunar porosity of the cortical bone and tibia load-related changes in trabecular and cortical bone were measured by nanoCT and micro-CT, respectively. We found that the mean skeletal perfusion (loaded: non-loaded limb ratio) increased by 56\% immediately following the first loading episode (versus baseline, P}, issn = {8750-7587}, journal = {Journal of Applied Physiology}, publicationstatus = {Published}, publisher = {American Physiological Society}, url = {https://rvc-repository.worktribe.com/output/1378225}, keyword = {ePrints migration}, year = {2020}, author = {Gohin, S and Javaheri, B and Hopkinson, M and Pitsillides, A A and Arnett, T R and Chenu, Chantal} }