@article { , title = {Epidemiological prevalence of phenotypical resistances and mobilised colistin resistance in avian commensal and pathogenic E. coli from Denmark, France, Netherlands and UK}, abstract = {Colistin has been used for treatment of non-invasive gastrointestinal infections caused by avian pathogenic E. coli (APEC). The discovery of mobilised colistin resistance (mcr) in E. coli [1] has instigated a One Health approach to minimise colistin use and the spread of resistance. The aim of this study was to compare colistin susceptibility of APECs (collected from Denmark n=25 and France n=39) versus commensal E. coli (collected from the Netherlands n=51 and the UK n=60), alongside genetic (mcr-1-5) and phenotypic resistance against six other antimicrobial classes (aminoglycosides, cephalosporins, fluoroquinolones, penicillins, sulphonamides/trimethoprim, tetracyclines). Minimum inhibitory concentration (MIC) values were determined using a broth microdilution method (EUCAST guidelines), phenotypic resistance was determined via disk diffusion. Colistin MIC of APEC were significantly lower than for commensals by 1 dilution (p < 0.0001, Anderson-Darling test) and differences in distributions were observed between countries. No isolate carried mcr-1-5. Three phenotypically resistant isolates were identified in 2/62 APEC and 1/111 commensal isolates. Gentamicin or gentamicin-ceftriaxone co-resistance was observed in two of these isolates. This study showed a low prevalence of phenotypic colistin resistance with no apparent difference in colistin resistance between commensal E. coli strains and APEC strains.}, doi = {10.3390/antibiotics11050631}, journal = {Antibiotics (special edition) - Epidemiology, Impact and Mitigation of Antimicrobial Resistance in Veterinary Medicine}, publicationstatus = {Published}, publisher = {MDPI}, year = {2022}, author = {Mead, Andrew and Billon-Lotz, Candice and Olsen, Rikke and Swift, Ben and Richez, Pascal and Stabler, Richard and Pelligand, Ludovic} }