Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella

Marugan-Hernandez, V; Fiddy, R; Nurse-Francis, J; Smith, O; Pritchard, L; Tomley, F M

Abstract

In this study, we evaluated the binding capacity of Type I MAR domains from novel E. tenella MCPs. Variants of the previously described HxT motif were analysed showing that HxT and VxT variants bind, whereas HxS and YxE variants did not. One of these MCP containing a single MAR (EtMCP2) showed an apical localization when expressed as a fusion with the fluorescent reporter mCherry in transgenic populations and a similar pattern of transcripts per zoite during endogenous development in vitro as the well-characterised microneme protein EtMIC2.

Citation

Marugan-Hernandez, V., Fiddy, R., Nurse-Francis, J., Smith, O., Pritchard, L., & Tomley, F. M. (2017). Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella. https://doi.org/10.1186/s13071-017-2454-4

• Journal Article Type: Article
• Acceptance Date: Oct 5, 2017
• Publication Date: Oct 17, 2017
• Deposit Date: Nov 2, 2017
• Publicly Available Date: Nov 2, 2017
• Journal: Parasites & Vectors
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 491
• DOI: https://doi.org/10.1186/s13071-017-2454-4
• Public URL: https://rvc-repository.worktribe.com/output/1390292