Musculoskeletal magnetic resonance imaging in the DE50-MD dog model of Duchenne muscular dystrophy

Hornby, NL; Drees, R; Harron, R; Chang, R; Wells, DJ; Piercy, RJ

Abstract

The DE50-MD canine model of Duchenne muscular dystrophy (DMD) has a dystrophin gene splice site mutation causing deletion of exon 50, an out-of-frame transcript and absence of dystrophin expression in striated muscles. We hypothesized that the musculoskeletal phenotype of DE50-MD dogs could be detected using Magnetic Resonance Imaging (MRI), that it would progress with age and that it would reflect those in other canine models and DMD patients. 15 DE50-MD and 10 age-matched littermate wild type (WT) male dogs underwent MRI every 3 months from 3 to 18 months of age. Normalized muscle volumes, global muscle T2 and ratio of post-to pre-gadolinium T1-weighted SI were evaluated in 7 pelvic limb and 4 lumbar muscles bilaterally. DE50-MD dogs, compared to WT, had smaller volumes in all muscles, except the cranial sartorius; global muscle T2 was significantly higher in DE50-MD dogs compared to WT. Muscle volumes plateaued and global muscle T2 decreased with age. Normalized muscle volumes and global muscle T2 revealed significant differences between groups longitudinally and should be useful to determine efficacy of therapeutics in this model with suitable power and low sample sizes. Musculoskeletal changes reflect those of DMD patients and other dog models. (c) 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )

Citation

Hornby, N., Drees, R., Harron, R., Chang, R., Wells, D., & Piercy, R. (2021). Musculoskeletal magnetic resonance imaging in the DE50-MD dog model of Duchenne muscular dystrophy. Neuromuscular Disorders, 31(8), 736-751. https://doi.org/10.1016/j.nmd.2021.05.010

• Journal Article Type: Article
• Acceptance Date: May 26, 2021
• Publication Date: 2021
• Deposit Date: Nov 25, 2021
• Publicly Available Date: Nov 25, 2021
• Print ISSN: 0960-8966
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 31
• Issue: 8
• Pages: 736-751
• DOI: https://doi.org/10.1016/j.nmd.2021.05.010
• Keywords: DMD; DE50-MD; Imaging biomarkers; MRI; Musculoskeletal; CRANIAL SARTORIUS MUSCLE; SKELETAL-MUSCLE; DISEASE PROGRESSION; MRI; FAT; QUANTIFICATION; GADOLINIUM; INFILTRATION; HYPERTROPHY; GADODIAMIDE
• Public URL: https://rvc-repository.worktribe.com/output/1552723