F Soldevila
Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus
Soldevila, F; Edwards, JC; Graham, SP; Crooke, HR; Werling, D; Steinbach, F
Authors
JC Edwards
SP Graham
HR Crooke
D Werling
F Steinbach
Abstract
Classical swine fever (CSF) is a highly contagious disease caused by the classical swine fever virus (CSFV). The live attenuated C-strain vaccine is highly efficacious, initiating protection within several days of delivery. The vaccine strain is detected in the tonsil early after inoculation, yet little is known of the role that tonsillar immune cells might play in initiating protection. Comparing the C-strain vaccine with the pathogenic CSFV Alfort-187 strain, changes in the myeloid cell compartment of the tonsil were observed. CSFV infection led to the emergence of an additional CD163(+)CD14(+) cell population, which showed the highest levels of Alfort-187 and C-strain infection. There was also an increase in both the frequency and activation status (as shown by increased MHC-II expression) of the tonsillar conventional dendritic cells 1 (cDC1) in pigs inoculated with the C-strain. Notably, the activation of cDC1 cells coincided in time with the induction of a local CSFV-specific IFN-gamma(+) CD8 T cell response in C-strain vaccinated pigs, but not in pigs that received Alfort-187. Moreover, the frequency of CSFV-specific IFN-gamma(+) CD8 T cells was inversely correlated to the viral load in the tonsils of individual animals. Accordingly, we hypothesise that the activation of cDC1 is key in initiating local CSFV-specific CD8 T cell responses which curtail early virus replication and dissemination.
Citation
Soldevila, F., Edwards, J., Graham, S., Crooke, H., Werling, D., & Steinbach, F. (2021). Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus. International Journal of Molecular Sciences, 22(16), https://doi.org/10.3390/ijms22168795
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 1, 2020 |
Publication Date | 2021 |
Deposit Date | Jan 5, 2022 |
Publicly Available Date | Jan 5, 2022 |
Print ISSN | 1661-6596 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 22 |
Issue | 16 |
DOI | https://doi.org/10.3390/ijms22168795 |
Keywords | dendritic cells; macrophages; myeloid; palatine tonsil; pig; CSFV; LYMPHOCYTE APOPTOSIS; BLOOD; MACROPHAGES; MONOCYTES; PATHOGENESIS; EXPRESSION; INFECTION; EPIDEMIC; SAMPLES; TISSUE |
Public URL | https://rvc-repository.worktribe.com/output/1554121 |
Files
OA
(3.3 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
You might also like
First detection and characterisation of Eimeria zaria in Europe
(2023)
Journal Article
East Coast fever, a neglected tropical disease with an outdated vaccine approach?
(2022)
Journal Article
Downloadable Citations
About RVC Repository
Administrator e-mail: publicationsrepos@rvc.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search