p Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little variation

BRaDP1T Consortium; Bosse, JT; Li, YW; Cohen, LM; Stegger, M; Angen, O; Lacouture, S; Gottschalk, M; Lei, LC; Koene, M; Kuhnert, P; Bandara, AB; Inzana, TJ; Holden, MTG; Harris, D; Oshota, O; Maskell, DJ; Tucker, AW; Wren, BW; Rycroft, AN; Langford, PR

Abstract

We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.

Citation

BRaDP1T Consortium, Bosse, J., Li, Y., Cohen, L., Stegger, M., Angen, O., Lacouture, S., Gottschalk, M., Lei, L., Koene, M., Kuhnert, P., Bandara, A., Inzana, T., Holden, M., Harris, D., Oshota, O., Maskell, D., Tucker, A., Wren, B., Rycroft, A., & Langford, P. (2021). p Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little variation. Microbial Genomics, 7(11), https://doi.org/10.1099/mgen.0.000687

• Journal Article Type: Article
• Acceptance Date: Sep 8, 2021
• Publication Date: Nov 24, 2021
• Deposit Date: Jul 25, 2023
• Publicly Available Date: Jul 25, 2023
• Journal: Microbial Genomics
• Electronic ISSN: 2057-5858
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 11
• DOI: https://doi.org/10.1099/mgen.0.000687
• Keywords: Actinobacillus pleuropneumoniae; serovar 8 reference genome; SNPs; HAEMOPHILUS-PLEUROPNEUMONIAE; SEROLOGICAL CHARACTERIZATION; NUCLEOTIDE-SEQUENCE; SEROTYPE; IDENTIFICATION; PLASMIDS; PROPOSAL; LOCI; DEGS