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Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation (2017)
Journal Article
Staines, K. A., Javaheri, B., Hohenstein, P., Fleming, R., Ikpegbu, E., Unger, E., …Farquharson, C. (2017). Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation. Journal of Cellular Physiology, 232(11), 3006-3019. https://doi.org/10.1002/jcp.25999

The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mechanisms are still unknown. Due to the known embryonic lethality induced by gl... Read More about Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation.

Detection of early osteogenic commitment in primary cells using Raman spectroscopy (2017)
Journal Article
Smith, S. J., Emery, R., Pitsillides, A. A., Clarkin, C. E., & Mahajan, S. (2017). Detection of early osteogenic commitment in primary cells using Raman spectroscopy. Analyst, 142(11), 1962-1973. https://doi.org/10.1039/C6AN02469F

Major challenges in the development of novel implant surfaces for artificial joints include osteoblast heterogeneity and the lack of a simple and sensitive in vitro assay to measure early osteogenic responses. Raman spectroscopy is a label-free, non-... Read More about Detection of early osteogenic commitment in primary cells using Raman spectroscopy.

The role of embryo movement in the development of the furcula (2017)
Journal Article
Pollard, A. S., Boyd, S., McGonnell, I. M., & Pitsillides, A. A. (2017). The role of embryo movement in the development of the furcula. Journal of Anatomy, 230(3), 435-443. https://doi.org/10.1111/joa.12571

The pectoral girdle is a complex structure which varies in its morphology between species. A major component in birds is the furcula, which can be considered equivalent to a fusion of the paired clavicles found in many mammals, and the single intercl... Read More about The role of embryo movement in the development of the furcula.

Limb proportions show developmental plasticity in response to embryo movement (2017)
Journal Article
Pollard, A. S., Charlton, B. G., Hutchinson, J. R., Gustafsson, T., McGonnell, I. M., Timmons, J. A., & Pitsillides, A. A. (2017). Limb proportions show developmental plasticity in response to embryo movement. https://doi.org/10.1038/srep41926

Animals have evolved limb proportions adapted to different environments, but it is not yet clear to what extent these proportions are directly influenced by the environment during prenatal development. The developing skeleton experiences mechanical l... Read More about Limb proportions show developmental plasticity in response to embryo movement.

Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues (2017)
Journal Article
Al-Jazzar, A., Javaheri, B., Prideaux, M., Boyde, A., Scudamore, C. L., Cherifi, C., …Pitsillides, A. A. (2017). Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues. International Journal of Molecular Sciences, 18(1), https://doi.org/10.3390/ijms18010029

Mice harbouring a dentin matrix protein 1 (Dmp1) promoter-driven human diphtheria toxin (DT) receptor (HDTR) transgene (Tg) have recently been used to attain targeted ablation of osteocytes by diphtheria toxin (DT) treatment in order to define osteoc... Read More about Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues.

E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models (2016)
Journal Article
Staines, K. A., Prideaux, M., Allen, S. P., Buttle, D. J., Pitsillides, A. A., & Farquharson, C. (2016). E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models. Journal of Cellular Physiology, 231(6), 1392-1404. https://doi.org/10.1002/jcp.25282

The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are still unknown. Using the late osteoblast MLO-A5 cell line we reveal increased E... Read More about E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models.

Predicting cortical bone adaptation to axial loading in the mouse tibia (2015)
Journal Article
Pereira, A. F., Javaheri, B., Pitsillides, A. A., & Shefelbine, S. J. (2015). Predicting cortical bone adaptation to axial loading in the mouse tibia. https://doi.org/10.1098/rsif.2015.0590

The development of predictive mathematical models can contribute to a deeper understanding of the specific stages of bone mechanobiology and the process by which bone adapts to mechanical forces. The objective of this work was to predict, with spatia... Read More about Predicting cortical bone adaptation to axial loading in the mouse tibia.

Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing (2015)
Journal Article
Javaheri, B., Carriero, A., Staines, K. A., Chang, Y. M., Houston, D. A., Oldknow, K. J., …Pitsillides, A. A. (2015). Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing. BONE, 81, 277-291. https://doi.org/10.1016/j.bone.2015.07.035

PHOSPHO1 is one of principal proteins involved in initiating bone matrix mineralisation. Recent studies have found that Phospho1 KO mice (Phospho1-R74X) display multiple skeletal abnormalities with spontaneous fractures, bowed long bones, osteomalaci... Read More about Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing.

Occipital foramina development involves localised regulation of mesenchyme proliferation and is independent of apoptosis (2015)
Journal Article
Akbareian, S. E., Pitsillides, A. A., Macharia, R. G., & McGonnell, I. M. (2015). Occipital foramina development involves localised regulation of mesenchyme proliferation and is independent of apoptosis. Journal of Anatomy, 226(6), 560-574. https://doi.org/10.1111/joa.12304

Cranial foramina are holes within the skull, formed during development, allowing entry and exit of blood vessels and nerves. Once formed they must remain open, due to the vital structures they contain, i.e. optic nerves, jugular vein, carotid artery,... Read More about Occipital foramina development involves localised regulation of mesenchyme proliferation and is independent of apoptosis.

Local origins impart conserved bone type-related differences in human osteoblast behaviour (2015)
Journal Article
Shah, M., Gburcik, V., Reilly, P., Sankey, R. A., Emery, R. J., Clarkin, C. E., & Pitsillides, A. A. (2015). Local origins impart conserved bone type-related differences in human osteoblast behaviour. https://doi.org/10.22203/eCM.v029a12

Osteogenic behaviour of osteoblasts from trabecular, cortical and subchondral bone were examined to determine any bone type-selective differences in samples from both osteoarthritic (OA) and osteoporotic (OP) patients. Cell growth, differentiation; a... Read More about Local origins impart conserved bone type-related differences in human osteoblast behaviour.

Structure, ontogeny and evolution of the patellar tendon in emus (Dromaius novaehollandiae) and other palaeognath birds (2015)
Journal Article
Regnault, S., Pitsillides, A. A., & Hutchinson, J. R. (2015). Structure, ontogeny and evolution of the patellar tendon in emus (Dromaius novaehollandiae) and other palaeognath birds. https://doi.org/10.7717/peerj.711

The patella (kneecap) exhibits multiple evolutionary origins in birds, mammals, and lizards, and is thought to increase the mechanical advantage of the knee extensor muscles. Despite appreciable interest in the specialized anatomy and locomotion of p... Read More about Structure, ontogeny and evolution of the patellar tendon in emus (Dromaius novaehollandiae) and other palaeognath birds.