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Proportion of concentrate in the diet of early lactation dairy cows has contrasting effects on circulating leukocyte global transcriptomic profiles, health and fertility according to parity (2022)
Journal Article
Cheng, Z., McLaughlin, D., Little, M., Ferris, C., Salavati, M., Ingvartsen, K., …Wathes, D. C. (2022). Proportion of concentrate in the diet of early lactation dairy cows has contrasting effects on circulating leukocyte global transcriptomic profiles, health and fertility according to parity. International Journal of Molecular Sciences, https://doi.org/10.3390/ijms24010039

The functionality of circulating leukocytes in dairy cows is suppressed after calving, with negative energy balance as a risk factor. Leukocyte transcriptomic profiles were compared separately in 44 multiparous (MP) and 18 primiparous (PP) Holstein–F... Read More about Proportion of concentrate in the diet of early lactation dairy cows has contrasting effects on circulating leukocyte global transcriptomic profiles, health and fertility according to parity.

Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows (2022)
Journal Article
Cheng, Z., Palma-Vera, S., Buggiotti, L., Salavati, M., Becker, F., Werling, D., …consortium, G. (in press). Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows. Animals, 12(16), 2146. https://doi.org/10.3390/ani12162146

The risk and severity of clinical infection with Escherichia coli as a causative pathogen for bovine mastitis is influenced by the hosts’ phenotypic and genotypic variables. We used RNA-Seq analysis of circulating leukocytes to investigate global tra... Read More about Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows.

Associations between Circulating IGF-1 Concentrations and Disease Status with the Leukocyte Transcriptome in Early Lactation Dairy Cows (2021)
Journal Article
Wathes, D. C., Becker, F., Buggiotti, L., Crowe, M. A., Ferris, C., Foldager, L., …GplusE consortium. (2021). Associations between Circulating IGF-1 Concentrations and Disease Status with the Leukocyte Transcriptome in Early Lactation Dairy Cows. Animals,

Peripartum dairy cows commonly experience negative energy balance (EB) and immunosuppression together with high incidences of infectious and metabolic disease. This study investigated mechanisms linking EB status with immune defence in early lactatio... Read More about Associations between Circulating IGF-1 Concentrations and Disease Status with the Leukocyte Transcriptome in Early Lactation Dairy Cows.

A survey of calf management practices and farmer perceptions of calf housing in UK dairy herds (2021)
Journal Article
Mahendran, S., Wathes, C., Booth, R., & Blackie, N. (2021). A survey of calf management practices and farmer perceptions of calf housing in UK dairy herds. Journal of Dairy Science, https://doi.org/10.3168/jds.2021-20638

Adoption of optimal management techniques for rearing dairy calves has significant impacts on their health, welfare and productivity. Despite much published literature on best practice, calf morbidity and mortality rates remain high. This survey aime... Read More about A survey of calf management practices and farmer perceptions of calf housing in UK dairy herds.

VCF Files (2021)
Dataset
Pollott, G., Wathes, D. C., Piercy, R., Massey, C., Salavati, M., & Cheng, Z. VCF Files. [Dataset]

Comparison of the transcriptome in circulating leukocytes in early lactation between primiparous and multiparous cows provides evidence for age-related changes (2021)
Journal Article
Buggiotti, L., Cheng, Z., Salavati, M., Wathes, C., & GplusE Consortium. (2021). Comparison of the transcriptome in circulating leukocytes in early lactation between primiparous and multiparous cows provides evidence for age-related changes. BMC Genomics, https://doi.org/10.1186/s12864-021-07977-5

Background: Previous studies have identified many immune pathways which are consistently altered in humans and model organisms as they age. Dairy cows are often culled at quite young ages due to an inability to cope adequately with metabolic and infe... Read More about Comparison of the transcriptome in circulating leukocytes in early lactation between primiparous and multiparous cows provides evidence for age-related changes.

Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis (2021)
Journal Article
Cheng, Z., Buggiotti, L., Salavati, M., Marchitelli, C., Palma-Vera, S., Wylie, A., …GplusE Consortium. (in press). Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis. Molecular Biology Reports,

Bovine mastitis is an inflammatory disease of the mammary gland, which is classified as subclinical or clinical. Circulating neutrophils are recruited to the udder to help combat infection. We compared the transcriptomic profiles in circulating leuko... Read More about Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis.

Comparison of the Ability of High and Low Virulence Strains of noncytopathic Bovine Viral Diarrhoea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium (2021)
Journal Article
Cheng, Z., Wang, K., Thomas, C., Zhang, S., & Wathes, D. C. (2021). Comparison of the Ability of High and Low Virulence Strains of noncytopathic Bovine Viral Diarrhoea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium. Frontiers in Veterinary Science,

Bovine Viral Diarrhoea virus (BVDV) is a pestivirus with a single-stranded, positive sense RNA genome. It is endemic in many cattle populations, causing major economic losses in part due to reduced fertility. BVDV exhibits great genetic diversity an... Read More about Comparison of the Ability of High and Low Virulence Strains of noncytopathic Bovine Viral Diarrhoea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium.

The Health and Behavioural Effects of Individual versus Pair Housing of Calves at Different Ages on a UK Commercial Dairy Farm (2021)
Journal Article
Mahendran, S., Blackie, N., Booth, R., & Wathes, C. The Health and Behavioural Effects of Individual versus Pair Housing of Calves at Different Ages on a UK Commercial Dairy Farm. Manuscript submitted for publication

Housing management of dairy calves is one of the factors that contributes to a successful rearing outcome. Individual housing of pre-weaned calves is thought to provide enhanced biosecurity and easier monitoring of the individual, and so remains prev... Read More about The Health and Behavioural Effects of Individual versus Pair Housing of Calves at Different Ages on a UK Commercial Dairy Farm.

Effect of diet and non-esterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows (2021)
Dataset
Cheng, Z., Wylie, A., Ferris, C., Ingvartsen, K., Wathes, D. C., & Consortium, G. Effect of diet and non-esterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows. [Dataset]. https://doi.org/10.34840/gsy7-nc21

After calving, lipid mobilization caused by increased nutrient demands for lactation leads to elevated circulating concentrations of non-esterified fatty acids (NEFA). Excessive NEFA levels have previously been identified as a major risk factor for p... Read More about Effect of diet and non-esterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows.

Mining the Unmapped Reads in Bovine RNA-Seq Data Reveals the Prevalence of Bovine Herpes Virus-6 in European Dairy Cows and the Associated Changes in Their Phenotype and Leucocyte Transcriptome (2020)
Journal Article
Buggiotti, L., Cheng, Z., Wathes, C., & GplusE Consortium, G. (in press). Mining the Unmapped Reads in Bovine RNA-Seq Data Reveals the Prevalence of Bovine Herpes Virus-6 in European Dairy Cows and the Associated Changes in Their Phenotype and Leucocyte Transcriptome. Viruses, 12(12), 1451. https://doi.org/10.3390/v12121451

Microbial RNA is detectable in host samples by aligning unmapped reads from RNA sequencing against taxon reference sequences, generating a score proportional to the microbial load. An RNA-Seq data analysis showed that 83.5% of leukocyte samples from... Read More about Mining the Unmapped Reads in Bovine RNA-Seq Data Reveals the Prevalence of Bovine Herpes Virus-6 in European Dairy Cows and the Associated Changes in Their Phenotype and Leucocyte Transcriptome.

Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation (2020)
Dataset
Cheng, Z., Wathes, D. C., Buggiotti, L., Salavati, M., Takeda, H., Tang, L., …Crowe, M. A. Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation. [Dataset]. https://doi.org/10.34840/q4vd-m506

Homeorhetic mechanisms assist dairy cows to transition from pregnancy to lactation. Less successful cows develop severe negative energy balance (NEB), placing them at risk of metabolic and infectious disease and reduced fertility. We have previously... Read More about Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation.