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Stable Sulforaphane Targets the Early Stages of Osteoclast Formation to Engender a Lasting Functional Blockade of Osteoclastogenesis (2024)
Journal Article
Louka, P., Orriss, I. R., & Pitsillides, A. A. (2024). Stable Sulforaphane Targets the Early Stages of Osteoclast Formation to Engender a Lasting Functional Blockade of Osteoclastogenesis. Cells, 13(2), https://doi.org/10.3390/cells13020165

Sulforaphane, the native but unstable form of SFX-01, is an antioxidant that activates the NRF2 and inhibits the NF-KB pathways to achieve its actions. Resolving the mechanism(s) by which SFX-01 serves to control the various osteoclastogenic stages m... Read More about Stable Sulforaphane Targets the Early Stages of Osteoclast Formation to Engender a Lasting Functional Blockade of Osteoclastogenesis.

Deletion of the P2Y(2) receptor aggravates internal elastic lamina calcification in chronic kidney disease mice through upregulation of alkaline phosphatase and lipocalin-2 (2022)
Journal Article
Opdebeeck, B., Huysmans, I., van den Branden, A., Orriss, I., D'Haese, P., & Verhulst, A. (2023). Deletion of the P2Y(2) receptor aggravates internal elastic lamina calcification in chronic kidney disease mice through upregulation of alkaline phosphatase and lipocalin-2. FASEB Journal, 37(1), https://doi.org/10.1096/fj.202201044R

Calcification of the medial layer, inducing arterial stiffness, contributes significantly to cardiovascular mortality in patients with chronic kidney disease (CKD). Extracellular nucleotides block the mineralization of arteries by binding to purinerg... Read More about Deletion of the P2Y(2) receptor aggravates internal elastic lamina calcification in chronic kidney disease mice through upregulation of alkaline phosphatase and lipocalin-2.

Glomerulonephritis and autoimmune vasculitis are independent of P2RX7 but may depend on alternative inflammasome pathways (2022)
Journal Article
Prendecki, M., McAdoo, S., Turner-Stokes, T., Garcia-Diaz, A., Orriss, I., Woollard, K., Behmoaras, J., Cook, H., Unwin, R., Pusey, C., Aitman, T., & Tam, F. (2022). Glomerulonephritis and autoimmune vasculitis are independent of P2RX7 but may depend on alternative inflammasome pathways. Journal of Pathology, 257(3), 300-313. https://doi.org/10.1002/path.5890

P2RX7, an ionotropic receptor for extracellular adenosine triphosphate (ATP), is expressed on immune cells, including macrophages, monocytes, and dendritic cells and is upregulated on nonimmune cells following injury. P2RX7 plays a role in many biolo... Read More about Glomerulonephritis and autoimmune vasculitis are independent of P2RX7 but may depend on alternative inflammasome pathways.

N-acetylcysteine (NAC) differentially affects arterial medial calcification and bone formation: the role of L-cysteine and hydrogen sulphide (2021)
Journal Article
Orriss, I., Bourne, L. E., Patel, J. J., Davies, B. K., Neven, E., Verhulst, A., D'Haese, P. C., PD Wheeler-Jones, C., & Orriss, I. R. (in press). N-acetylcysteine (NAC) differentially affects arterial medial calcification and bone formation: the role of L-cysteine and hydrogen sulphide. Journal of Cellular Physiology, https://doi.org/10.1002/jcp.30605

Arterial medial calcification (AMC) is the deposition of calcium phosphate in the arteries. AMC is widely thought to share similarities with physiological bone formation; however, emerging evidence suggests several key differences between these proce... Read More about N-acetylcysteine (NAC) differentially affects arterial medial calcification and bone formation: the role of L-cysteine and hydrogen sulphide.

Extracellular Nucleotides Regulate Arterial Calcification by Activating Both Independent and Dependent Purinergic Receptor Signaling Pathways (2020)
Journal Article
Opdebeeck, B., Orriss, I., Neven, E., D’Haese, P. C., & Verhulst, A. (2020). Extracellular Nucleotides Regulate Arterial Calcification by Activating Both Independent and Dependent Purinergic Receptor Signaling Pathways. International Journal of Molecular Sciences, 21(20), e7636

Arterial calcification, the deposition of calcium-phosphate crystals in the extracellular matrix, resembles physiological bone mineralization. It is well-known that extracellular nucleotides regulate bone homeostasis raising an emerging interest in t... Read More about Extracellular Nucleotides Regulate Arterial Calcification by Activating Both Independent and Dependent Purinergic Receptor Signaling Pathways.

Extracellular pyrophosphate: The body's “water softener” (2020)
Journal Article
Orriss, I. R. (2020). Extracellular pyrophosphate: The body's “water softener”. BONE, https://doi.org/10.1016/j.bone.2020.115243

Extracellular pyrophosphate (ePPi) was first identified as a key endogenous inhibitor of mineralisation in the 1960's by Fleisch and colleagues. The main source of ePPi seems to be extracellular ATP which is continually released from cells in a contr... Read More about Extracellular pyrophosphate: The body's “water softener”.

Inhibition of vascular smooth muscle cell calcification by ATP-analogues (2019)
Journal Article
Patel, J. J., Bourne, L. E., Millán, J. L., Arnett, T. R., MacRae, V. E., Wheeler-Jones, C. P. D., & Orriss, I. R. (2019). Inhibition of vascular smooth muscle cell calcification by ATP-analogues. Purinergic Signalling, 15(3), 315-326. https://doi.org/10.1007/s11302-019-09672-3

Arterial medial calcification (AMC) has been associated with phenotypic changes in vascular smooth muscle cells (VSMCs) that reportedly makes them more osteoblast-like. Previous work has shown that ATP/UTP can inhibit AMC directly via P2 receptors an... Read More about Inhibition of vascular smooth muscle cell calcification by ATP-analogues.

Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts (2019)
Journal Article
Patel, J. J., Bourne, L. E., Davies, B. K., Arnett, T. R., MacRae, V. E., Wheeler-Jones, C. P. D., & Orriss, I. R. (2019). Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts. Experimental Cell Research, 380(1), 100-113. https://doi.org/10.1016/j.yexcr.2019.04.020

Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, in the medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associated with the transdifferenti... Read More about Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts.

Inhibition of arterial medial calcification and bone mineralization by extracellular nucleotides: The same functional effect mediated by different cellular mechanisms (2018)
Journal Article
Patel, J. J., Zhu, D., Opdebeeck, B., D'Haese, P., Millán, J. L., Bourne, L. E., Wheeler-Jones, C. P. D., Arnett, T. R., MacRae, V. E., & Orriss, I. R. (2018). Inhibition of arterial medial calcification and bone mineralization by extracellular nucleotides: The same functional effect mediated by different cellular mechanisms. Journal of Cellular Physiology, 233(4), 3230-3243. https://doi.org/10.1002/jcp.26166

Arterial medial calcification (AMC) is thought to share some outward similarities to skeletal mineralization and has been associated with the transdifferentiation of vascular smooth muscle cells (VSMCs) to an osteoblast‐like phenotype. ATP and UTP ha... Read More about Inhibition of arterial medial calcification and bone mineralization by extracellular nucleotides: The same functional effect mediated by different cellular mechanisms.

Metabolic properties of the osteoclast (2017)
Journal Article
Arnett, T. R., & Orriss, I. R. (2017). Metabolic properties of the osteoclast. BONE, 115, 25-30. https://doi.org/10.1016/j.bone.2017.12.021

Osteoclasts are defined as cells capable of excavating 3-dimensional resorption pits in bone and other mineralised tissues. They are derived from the differentiation/fusion of promonocytic precursors, and are usually large, multinucleated cells. In c... Read More about Metabolic properties of the osteoclast.

Stable sulforaphane protects against gait anomalies and modifies bone microarchitecture in the spontaneous STR/Ort model of osteoarthritis (2017)
Journal Article
Javaheri, B., Poulet, B., Aljazzar, A., De Souza, R., Piles, M., Hopkinson, M., Shervill, E., Pollard, A. S., Chan, B., Chang, Y. M., Orriss, I., Lee, P., & Pitsillides, A. A. (2017). Stable sulforaphane protects against gait anomalies and modifies bone microarchitecture in the spontaneous STR/Ort model of osteoarthritis. BONE, 103, 308-317. https://doi.org/10.1016/j.bone.2017.07.028

Osteoarthritis (OA), affecting joints and bone, causes physical gait disability with huge socio-economic burden; treatment remains palliative. Roles for antioxidants in protecting against such chronic disorders have been examined previously. Sulforap... Read More about Stable sulforaphane protects against gait anomalies and modifies bone microarchitecture in the spontaneous STR/Ort model of osteoarthritis.

Activation of the P2Y2 receptor regulates bone cell function by enhancing ATP release (2017)
Journal Article
Orriss, I. R., Gueneri, D., Hajjawai, M. O., Shaw, K., Patel, J. J., & Arnett, T. R. (2017). Activation of the P2Y2 receptor regulates bone cell function by enhancing ATP release. Journal of Endocrinology, 233, 341-356. https://doi.org/10.1530/JOE-17-0042

Bone cells constitutively release ATP into the extracellular environment where it acts locally via P2 receptors to regulate bone cell function. Whilst P2Y2 receptor stimulation regulates bone mineralisation, the functional effects of this receptor in... Read More about Activation of the P2Y2 receptor regulates bone cell function by enhancing ATP release.