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Influence of the concentrate inclusion level in a grass silage-based diet on hepatic transcriptomic profiles in Holstein-Friesian dairy cows in early lactation (2023)
Journal Article
Cheng, Z., Little, M., Ferris, C., Takeda, H., Ingvartsen, K., Crowe, M., & Wathes, D. C. (2023). Influence of the concentrate inclusion level in a grass silage-based diet on hepatic transcriptomic profiles in Holstein-Friesian dairy cows in early lactation. Journal of Dairy Science, https://doi.org/10.3168/jds.2022-22860

Excessive negative energy balance (NEB) in early lactation is linked to an increased disease risk but may be mitigated by appropriate nutrition. The liver plays central roles in both metabolism and immunity. Hepatic transcriptomic profiles were compa... Read More about Influence of the concentrate inclusion level in a grass silage-based diet on hepatic transcriptomic profiles in Holstein-Friesian dairy cows in early lactation.

Hepatic global transcriptomic profiles of Holstein cows according to parity reveal age-related changes in early lactation (2023)
Journal Article
Cheng, Z., Ferris, C., Crowe, M., Ingvartsen, K., Grelet, C., Vanlierde, A., …Consortium, G. (2023). Hepatic global transcriptomic profiles of Holstein cows according to parity reveal age-related changes in early lactation. International Journal of Molecular Sciences, https://doi.org/10.3390/ijms24129906

Cows can live for over 20 years, but their productive lifespan averages only around 3 years after first calving. Liver dysfunction can reduce lifespan by increasing the risk of metabolic and infec-tious disease. This study investigated the changes in... Read More about Hepatic global transcriptomic profiles of Holstein cows according to parity reveal age-related changes in early lactation.

Proportion of concentrate in the diet of early lactation dairy cows has contrasting effects on circulating leukocyte global transcriptomic profiles, health and fertility according to parity (2022)
Journal Article
Cheng, Z., McLaughlin, D., Little, M., Ferris, C., Salavati, M., Ingvartsen, K., …Wathes, D. C. (2022). Proportion of concentrate in the diet of early lactation dairy cows has contrasting effects on circulating leukocyte global transcriptomic profiles, health and fertility according to parity. International Journal of Molecular Sciences, https://doi.org/10.3390/ijms24010039

The functionality of circulating leukocytes in dairy cows is suppressed after calving, with negative energy balance as a risk factor. Leukocyte transcriptomic profiles were compared separately in 44 multiparous (MP) and 18 primiparous (PP) Holstein–F... Read More about Proportion of concentrate in the diet of early lactation dairy cows has contrasting effects on circulating leukocyte global transcriptomic profiles, health and fertility according to parity.

Locating a novel autosomal recessive genetic variant in the cattle glucokinase gene using only WGS data from three cases and six carriers (2022)
Journal Article
Pollott, G. E., Piercy, R. J., Massey, C., Salavati, M., Cheng, Z., & Wathes, D. C. (2022). Locating a novel autosomal recessive genetic variant in the cattle glucokinase gene using only WGS data from three cases and six carriers. Frontiers in Genetics, 13, 14. https://doi.org/10.3389/fgene.2022.755693

New Mendelian genetic conditions, which adversely affect livestock, arise all the time. To manage them effectively, some methods need to be devised that are quick and accurate. Until recently, finding the causal genomic site of a new autosomal recess... Read More about Locating a novel autosomal recessive genetic variant in the cattle glucokinase gene using only WGS data from three cases and six carriers.

Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows (2022)
Journal Article
Cheng, Z., Palma-Vera, S., Buggiotti, L., Salavati, M., Becker, F., Werling, D., …consortium, G. (in press). Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows. Animals, 12(16), 2146. https://doi.org/10.3390/ani12162146

The risk and severity of clinical infection with Escherichia coli as a causative pathogen for bovine mastitis is influenced by the hosts’ phenotypic and genotypic variables. We used RNA-Seq analysis of circulating leukocytes to investigate global tra... Read More about Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows.

Associations between Circulating IGF-1 Concentrations and Disease Status with the Leukocyte Transcriptome in Early Lactation Dairy Cows (2021)
Journal Article
Wathes, D. C., Becker, F., Buggiotti, L., Crowe, M. A., Ferris, C., Foldager, L., …GplusE consortium. (2021). Associations between Circulating IGF-1 Concentrations and Disease Status with the Leukocyte Transcriptome in Early Lactation Dairy Cows. Animals,

Peripartum dairy cows commonly experience negative energy balance (EB) and immunosuppression together with high incidences of infectious and metabolic disease. This study investigated mechanisms linking EB status with immune defence in early lactatio... Read More about Associations between Circulating IGF-1 Concentrations and Disease Status with the Leukocyte Transcriptome in Early Lactation Dairy Cows.

VCF Files (2021)
Dataset
Pollott, G., Wathes, D. C., Piercy, R., Massey, C., Salavati, M., & Cheng, Z. VCF Files. [Dataset]

Comparison of the transcriptome in circulating leukocytes in early lactation between primiparous and multiparous cows provides evidence for age-related changes (2021)
Journal Article
Buggiotti, L., Cheng, Z., Salavati, M., Wathes, C., & GplusE Consortium. (2021). Comparison of the transcriptome in circulating leukocytes in early lactation between primiparous and multiparous cows provides evidence for age-related changes. BMC Genomics, https://doi.org/10.1186/s12864-021-07977-5

Background: Previous studies have identified many immune pathways which are consistently altered in humans and model organisms as they age. Dairy cows are often culled at quite young ages due to an inability to cope adequately with metabolic and infe... Read More about Comparison of the transcriptome in circulating leukocytes in early lactation between primiparous and multiparous cows provides evidence for age-related changes.

Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis (2021)
Journal Article
Cheng, Z., Buggiotti, L., Salavati, M., Marchitelli, C., Palma-Vera, S., Wylie, A., …GplusE Consortium. (in press). Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis. Molecular Biology Reports,

Bovine mastitis is an inflammatory disease of the mammary gland, which is classified as subclinical or clinical. Circulating neutrophils are recruited to the udder to help combat infection. We compared the transcriptomic profiles in circulating leuko... Read More about Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis.

Comparison of the Ability of High and Low Virulence Strains of noncytopathic Bovine Viral Diarrhoea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium (2021)
Journal Article
Cheng, Z., Wang, K., Thomas, C., Zhang, S., & Wathes, D. C. (2021). Comparison of the Ability of High and Low Virulence Strains of noncytopathic Bovine Viral Diarrhoea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium. Frontiers in Veterinary Science,

Bovine Viral Diarrhoea virus (BVDV) is a pestivirus with a single-stranded, positive sense RNA genome. It is endemic in many cattle populations, causing major economic losses in part due to reduced fertility. BVDV exhibits great genetic diversity an... Read More about Comparison of the Ability of High and Low Virulence Strains of noncytopathic Bovine Viral Diarrhoea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium.

Effect of diet and non-esterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows (2021)
Dataset
Cheng, Z., Wylie, A., Ferris, C., Ingvartsen, K., Wathes, D. C., & Consortium, G. Effect of diet and non-esterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows. [Dataset]. https://doi.org/10.34840/gsy7-nc21

After calving, lipid mobilization caused by increased nutrient demands for lactation leads to elevated circulating concentrations of non-esterified fatty acids (NEFA). Excessive NEFA levels have previously been identified as a major risk factor for p... Read More about Effect of diet and non-esterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows.

Mining the Unmapped Reads in Bovine RNA-Seq Data Reveals the Prevalence of Bovine Herpes Virus-6 in European Dairy Cows and the Associated Changes in Their Phenotype and Leucocyte Transcriptome (2020)
Journal Article
Buggiotti, L., Cheng, Z., Wathes, C., & GplusE Consortium, G. (in press). Mining the Unmapped Reads in Bovine RNA-Seq Data Reveals the Prevalence of Bovine Herpes Virus-6 in European Dairy Cows and the Associated Changes in Their Phenotype and Leucocyte Transcriptome. Viruses, 12(12), 1451. https://doi.org/10.3390/v12121451

Microbial RNA is detectable in host samples by aligning unmapped reads from RNA sequencing against taxon reference sequences, generating a score proportional to the microbial load. An RNA-Seq data analysis showed that 83.5% of leukocyte samples from... Read More about Mining the Unmapped Reads in Bovine RNA-Seq Data Reveals the Prevalence of Bovine Herpes Virus-6 in European Dairy Cows and the Associated Changes in Their Phenotype and Leucocyte Transcriptome.

Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation (2020)
Dataset
Cheng, Z., Wathes, D. C., Buggiotti, L., Salavati, M., Takeda, H., Tang, L., …Crowe, M. A. Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation. [Dataset]. https://doi.org/10.34840/q4vd-m506

Homeorhetic mechanisms assist dairy cows to transition from pregnancy to lactation. Less successful cows develop severe negative energy balance (NEB), placing them at risk of metabolic and infectious disease and reduced fertility. We have previously... Read More about Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation.

Decreasing of S100A4 in bovine endometritis in vivo and in vitro (2020)
Journal Article
Li, Y., Wang, D., Wu, X., He, B., Cheng, Z., Szenci, O., …Yan, Z. (2020). Decreasing of S100A4 in bovine endometritis in vivo and in vitro. Theriogenology, https://doi.org/10.1016/j.theriogenology.2020.05.015

Endometritis is a prevalent reproductive disease in dairy cows, and is a superficial inflammation of the endometrium. S100 calcium-binding protein A4 (S100A4) is suggested to be implicated in the progression of inflammation. However, to our knowledge... Read More about Decreasing of S100A4 in bovine endometritis in vivo and in vitro.

Mechanisms linking bovine viral diarrhea virus (BVDV) infection with infertility in cattle (2019)
Journal Article
Oguejiofor, C. F., Thomas, C. J., Cheng, Z. R., & Wathes, D. C. (2019). Mechanisms linking bovine viral diarrhea virus (BVDV) infection with infertility in cattle. Animal Health Research Reviews, https://doi.org/10.1017/S1466252319000057

Bovine viral diarrhea virus (BVDV) is an important infectious disease agent that causes significant reproductive and economic losses in the cattle industry worldwide. Although BVDV infection is known to cause poor fertility in cattle, a greater part... Read More about Mechanisms linking bovine viral diarrhea virus (BVDV) infection with infertility in cattle.

Importance of Viral Disease in Dairy Cow Fertility (2019)
Journal Article
Wathes, D. C., Oguejiofor, C. F., Tomas, C., & Cheng, Z. R. (2019). Importance of Viral Disease in Dairy Cow Fertility. Engineering, https://doi.org/10.1016/j.eng.2019.07.020

Many viral diseases are endemic in cattle populations worldwide. The ability of many viruses to cross the placenta and cause abortions and fetal malformations is well understood. There is also significant evidence that viral infections have additiona... Read More about Importance of Viral Disease in Dairy Cow Fertility.