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Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows (2022)
Journal Article
Cheng, Z., Palma-Vera, S., Buggiotti, L., Salavati, M., Becker, F., Werling, D., …consortium, G. (in press). Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows. Animals, 12(16), 2146. https://doi.org/10.3390/ani12162146

The risk and severity of clinical infection with Escherichia coli as a causative pathogen for bovine mastitis is influenced by the hosts’ phenotypic and genotypic variables. We used RNA-Seq analysis of circulating leukocytes to investigate global tra... Read More about Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows.

Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria (2022)
Journal Article
Gibson, A. J., Stiens, J., Passmore, I. J., Faulkner, V., Miculob, J., Willcocks, S., …Kendall, S. L. (2022). Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria. mBio, https://doi.org/10.1128/mbio.00672-22

Tuberculosis has severe impacts in both humans and animals. Understanding the genetic basis of survival of both Mycobacterium tuberculosis, the human adapted species, and Mycobacterium bovis, the animal adapted species is crucial to deciphering the b... Read More about Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria.

Development of a yeast-based vaccine for Theileria parva infection in cattle (2022)
Journal Article
Werling, D., Goh, S., Kolakowski, J., Holder, A., Pfuhl, M., Ngugi, D., …Werling, D. (2022). Development of a yeast-based vaccine for Theileria parva infection in cattle. npj Vaccines, https://doi.org/10.3389/fimmu.2021.674484

East Coast Fever (ECF), caused by the tick-borne apicomplexan parasite Theileria parva, remains one of the most important livestock diseases in sub-Saharan Africa with more than 1 million cattle dying from infection every year. Disease prevention rel... Read More about Development of a yeast-based vaccine for Theileria parva infection in cattle.

A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens (2022)
Journal Article
Soutter, F., Werling, D., Nolan, M., Küster, T., Attree, E., Marugán-Hernández, V., …Blake, D. (2022). A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens. Frontiers in Immunology, 13, Article 809711. https://doi.org/10.3389/fimmu.2022.809711

Cheap, easy-to-produce oral vaccines are needed for control of coccidiosis in chickens to reduce the impact of this disease on welfare and economic performance. Saccharomyces cerevisiae yeast expressing three Eimeria tenella antigens were developed a... Read More about A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens.

Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation (2021)
Journal Article
Queval, C., Fearns, A., Botella, L., Smyth, A., Schnettger, L., Mitermite, M., …Gutierrez, M. (2021). Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation. PLoS Pathogens, 17(3), https://doi.org/10.1371/journal.ppat.1009410

The Mycobacterium tuberculosis complex (MTBC) is a group of related pathogens that cause tuberculosis (TB) in mammals. MTBC species are distinguished by their ability to sustain in distinct host populations. While Mycobacterium bovis (Mbv) sustains t... Read More about Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation.

Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus (2021)
Journal Article
Soldevila, F., Edwards, J., Graham, S., Crooke, H., Werling, D., & Steinbach, F. (2021). Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus. International Journal of Molecular Sciences, 22(16), https://doi.org/10.3390/ijms22168795

Classical swine fever (CSF) is a highly contagious disease caused by the classical swine fever virus (CSFV). The live attenuated C-strain vaccine is highly efficacious, initiating protection within several days of delivery. The vaccine strain is dete... Read More about Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus.

Splenic Nerve Neuromodulation Reduces Inflammation and Promotes Resolution in Chronically Implanted Pigs (2021)
Journal Article
Sokal, D., McSloy, A., Donega, M., Kirk, J., Colas, R., Dolezalova, N., …Perkins, J. (2021). Splenic Nerve Neuromodulation Reduces Inflammation and Promotes Resolution in Chronically Implanted Pigs. Frontiers in Immunology, 12, https://doi.org/10.3389/fimmu.2021.649786

Neuromodulation of the immune system has been proposed as a novel therapeutic strategy for the treatment of inflammatory conditions. We recently demonstrated that stimulation of near-organ autonomic nerves to the spleen can be harnessed to modulate t... Read More about Splenic Nerve Neuromodulation Reduces Inflammation and Promotes Resolution in Chronically Implanted Pigs.

Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle (2021)
Journal Article
Goh, S., Kolakowski, J., Holder, A., Pfuhl, M., Ngugi, D., Ballingall, K., …Werling, D. (2021). Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle. Frontiers in Immunology, 12, https://doi.org/10.3389/fimmu.2021.674484

East Coast Fever (ECF), caused by the tick-borne apicomplexan parasite Theileria parva, remains one of the most important livestock diseases in sub-Saharan Africa with more than 1 million cattle dying from infection every year. Disease prevention rel... Read More about Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle.

Bovine Neutrophils Release Extracellular Traps and Cooperate With Macrophages in Mycobacterium avium subsp. paratuberculosis clearance In Vitro (2021)
Journal Article
Ladero-Aunon, I., Molina, E., Holder, A., Kolakowski, J., Harris, H., Urkitza, A., …Elguezabal, N. (2021). Bovine Neutrophils Release Extracellular Traps and Cooperate With Macrophages in Mycobacterium avium subsp. paratuberculosis clearance In Vitro. Frontiers in Immunology, 12, https://doi.org/10.3389/fimmu.2021.645304

Mycobacterium avium subsp. paratuberculosis (Map) is the underlying pathogen causing bovine paratuberculosis (PTB), an enteric granulomatous disease that mainly affects ruminants and for which an effective treatment is needed. Macrophages are the pri... Read More about Bovine Neutrophils Release Extracellular Traps and Cooperate With Macrophages in Mycobacterium avium subsp. paratuberculosis clearance In Vitro.

Human-relevant near-organ neuromodulation of the immune via the nerve (2021)
Journal Article
Donega, M., Fjordbakk, C., Kirk, J., Sokal, D., Gupta, I., Hunsberger, G., …Perkins, J. (2021). Human-relevant near-organ neuromodulation of the immune via the nerve. Proceedings of the National Academy of Sciences, 118(20), https://doi.org/10.1073/pnas.2025428118

Neuromodulation of immune function by stimulating the autonomic connections to the spleen has been demonstrated in rodent models. Consequently, neuroimmune modulation has been proposed as a new therapeutic strategy for the treatment of inflammatory c... Read More about Human-relevant near-organ neuromodulation of the immune via the nerve.

Probing differences in gene essentiality between the human and animal adapted (2021)
Journal Article
Gibson, A. J., Passmore, I. J., Faulkner, V., Xia, D., Nobeli, I., Stiens, J., …Kendall, S. L. (2021). Probing differences in gene essentiality between the human and animal adapted. Frontiers in Veterinary Science,

Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences and phenotypes despite being >99% identical at the nucleic acid level. Previous studies have explored gene expression changes between the members, ho... Read More about Probing differences in gene essentiality between the human and animal adapted.

Use of precision-cut tissue slices as a translational model to study host-pathogen and vaccine assessment (2021)
Journal Article
Majorova, D., Atkins, E., Martineau, H., Vokral, I., Oosterhuis, D., Olinga, P., …Werling, D. Use of precision-cut tissue slices as a translational model to study host-pathogen and vaccine assessment. Manuscript submitted for publication

The recent increase in new technologies to analyse host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there i... Read More about Use of precision-cut tissue slices as a translational model to study host-pathogen and vaccine assessment.

Impact of Eimeria tenella oocyst dose on parasite replication, lesion score and cytokine transcription in the caeca in three breeds of commercial layer chickens (2021)
Journal Article
Soutter, F., Werling, D., Tomley, F., Kim, S., Tomley, F. M., Blake, D., …Marugan-Hernandez, V. (in press). Impact of Eimeria tenella oocyst dose on parasite replication, lesion score and cytokine transcription in the caeca in three breeds of commercial layer chickens. Frontiers in Veterinary Science,

Eimeria species parasites infect the gastrointestinal tract of chickens, causing disease and impacting on production. The poultry industry relies on anticoccidial drugs and live vaccines to control Eimeria and there is a need for novel, scalable alte... Read More about Impact of Eimeria tenella oocyst dose on parasite replication, lesion score and cytokine transcription in the caeca in three breeds of commercial layer chickens.

Association of immune responses of Zebu and Holstein?Friesian cattle and resistance to mycobacteria in a BCG challenge model (2020)
Journal Article
Alcaraz‐López, O. A., Flores‐Villalva, S., Cortéz‐Hernández, O., Vigueras‐Meneses, G., Carrisoza‐Urbina, J., Benítez‐Guzmán, A., …Gutiérrez‐Pabello, J. A. (in press). Association of immune responses of Zebu and Holstein‐Friesian cattle and resistance to mycobacteria in a BCG challenge model. Transboundary and Emerging Diseases, https://doi.org/10.1111/tbed.13939

Enterobactin Deficiency in a Coliform Mastitis Isolate Decreases Its Fitness in a Murine Model: A Preliminary Host–Pathogen Interaction Study (2020)
Journal Article
Vander Elst, N., Breyne, K., Steenbrugge, J., Gibson, A. J., Smith, D. G. E., Germon, P., …Meyer, E. (in press). Enterobactin Deficiency in a Coliform Mastitis Isolate Decreases Its Fitness in a Murine Model: A Preliminary Host–Pathogen Interaction Study. Frontiers in Veterinary Science, 7, https://doi.org/10.3389/fvets.2020.576583

Escape of TLR5 Recognition by Leptospira spp.: A Rationale for Atypical Endoflagella (2020)
Journal Article
Holzapfel, M., Bonhomme, D., Cagliero, J., Vernel-Pauillac, F., Fanton d’Andon, M., Bortolussi, S., …Werts, C. (2020). Escape of TLR5 Recognition by Leptospira spp.: A Rationale for Atypical Endoflagella. Frontiers in Immunology, 11,

Leptospira (L.) interrogans are invasive bacteria responsible for leptospirosis, a worldwide zoonosis. They possess two periplasmic endoflagellae that allow their motility. L. interrogans are stealth pathogens that escape the innate immune recognitio... Read More about Escape of TLR5 Recognition by Leptospira spp.: A Rationale for Atypical Endoflagella.

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis (2020)
Journal Article
Holder, A., Garty, R., Elder, C., Mesnard, P., Laquerbe, C., Bartens, M., …Werling, D. (2020). Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis. Frontiers in Microbiology, 11, https://doi.org/10.3389/fmicb.2020.01420

Bovine tuberculosis (bTB), caused by Mycobacterium bovis, is a chronic zoonotic disease where host genetics is thought to contribute to susceptibility or resistance. One of the genes implicated is the SLC11A1 gene, that encodes for the natural resist... Read More about Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis.