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Efficacy of natural antimicrobial peptides versus peptidomimetic analogues: a systematic review (2022)
Journal Article
Hellewell, L., Gilani, N., Stanton, C., Pelligand, L., Franzyk, H., Guardabassi, L., & Good, L. (in press). Efficacy of natural antimicrobial peptides versus peptidomimetic analogues: a systematic review. Future Medicinal Chemistry, https://doi.org/10.4155/fmc-2022-0160

Aims: This systematic review was carried out to determine whether synthetic peptidomimetics exhibit significant advantages over antimicrobial peptides (AMPs) in terms of in vitro potency. Structural features - molecular weight, charge and length - we... Read More about Efficacy of natural antimicrobial peptides versus peptidomimetic analogues: a systematic review.

A Meta-Analysis to Estimate Prevalence of Resistance to Tetracyclines and Third Generation Cephalosporins in Enterobacteriaceae Isolated from Food Crops (2022)
Journal Article
Brunn, A., Roustit, M., Kadri-Alabi, Z., Guardabassi, L., & Waage, J. (2022). A Meta-Analysis to Estimate Prevalence of Resistance to Tetracyclines and Third Generation Cephalosporins in Enterobacteriaceae Isolated from Food Crops. Antibiotics, 11(10), https://doi.org/10.3390/antibiotics11101424

Application of human and animal waste to fields and water sources and on-farm antimicrobial usage are documented contributors to the occurrence of antimicrobial resistance (AMR) in agricultural domains. This meta-analysis aimed to determine the preva... Read More about A Meta-Analysis to Estimate Prevalence of Resistance to Tetracyclines and Third Generation Cephalosporins in Enterobacteriaceae Isolated from Food Crops.

Towards a Better and Harmonized Education in Antimicrobial Stewardship in European Veterinary Curricula (2021)
Journal Article
PREPARE-VET Working Grp, ESCMID Study Grp Vet Microbiology, ESCMID Study Grp Antimicrobial ste, Espinosa-Gongora, C., Jessen, L., Dyar, O., …Guardabassi, L. (2021). Towards a Better and Harmonized Education in Antimicrobial Stewardship in European Veterinary Curricula. Antibiotics, 10(4), https://doi.org/10.3390/antibiotics10040364

Education in antimicrobial stewardship (AMS) in veterinary medicine is essential to foster responsible antimicrobial use and control of antimicrobial resistance (AMR) in animals. AMS is listed by the EU and international organizations among the basic... Read More about Towards a Better and Harmonized Education in Antimicrobial Stewardship in European Veterinary Curricula.

Local and transboundary transmission of methicillin-resistant Staphylococcus aureus sequence type 398 through pig trading (2020)
Journal Article
Pirolo, M., Sieber, R. N., Moodley, A., Visaggio, D., Artuso, I., Gioffrè, A., …Visca, P. (2020). Local and transboundary transmission of methicillin-resistant Staphylococcus aureus sequence type 398 through pig trading. Applied and Environmental Microbiology, https://doi.org/10.1128/AEM.00430-20

Livestock-associated methicillin-resistant Staphylococcus aureus sequence type (ST) 398 (LA-MRSA ST398) is a genetic lineage for which pigs are regarded as the main reservoir. An increasing prevalence of LA-MRSA ST398 has been reported in areas with... Read More about Local and transboundary transmission of methicillin-resistant Staphylococcus aureus sequence type 398 through pig trading.

Specific staphylococcal cassette chromosome mec (SCCmec) types and clonal complexes are associated with low-level amoxicillin/clavulanic acid and cefalotin resistance in methicillin-resistant Staphylococcus pseudintermedius (2019)
Journal Article
Broens, E. M., Wagenaar, J. A., Duim, B., Mughini-Gras, L., Moodley, A., Guardabassi, L., …Wegener, A. (2019). Specific staphylococcal cassette chromosome mec (SCCmec) types and clonal complexes are associated with low-level amoxicillin/clavulanic acid and cefalotin resistance in methicillin-resistant Staphylococcus pseudintermedius. Journal of Antimicrobial Chemotherapy, 75(3), 508-511. https://doi.org/10.1093/jac/dkz509

Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius (2019)
Journal Article
Magnowska, Z., Jana, B., Brochmann, R. P., Hesketh, A., Lametsch, R., De Gobba, C., & Guardabassi, L. (2019). Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius. https://doi.org/10.1038/s41598-019-54091-4

We previously showed that doxycycline (DOX) and carprofen (CPF), a veterinary non-steroidal anti-inflammatory drug, have synergistic antimicrobial activity against methicillin-resistant Staphylococcus pseudintermedius (MRSP) carrying the tetracycline... Read More about Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius.

Impact of oral amoxicillin and amoxicillin/clavulanic acid treatment on bacterial diversity and ß-lactam resistance in the canine faecal microbiota (2019)
Journal Article
Espinosa-Gongora, C., Jessen, L. R., Kieler, I. N., Damborg, P., Bjørnvad, C. R., Gudeta, D. D., …Guardabassi, L. (2019). Impact of oral amoxicillin and amoxicillin/clavulanic acid treatment on bacterial diversity and ß-lactam resistance in the canine faecal microbiota. Journal of Antimicrobial Chemotherapy, 75(2), 351-361. https://doi.org/10.1093/jac/dkz458

EUCAST disk diffusion Criteria for the Detection of mecA-Mediated ß-lactam resistance in Staphylococcus pseudintermedius: oxacillin versus cefoxitin (2019)
Journal Article
Skov, R., Varga, A., Matuschek, E., Åhman, J., Bemis, D., Bengtsson, B., …Kahlmeter, G. (2019). EUCAST disk diffusion Criteria for the Detection of mecA-Mediated ß-lactam resistance in Staphylococcus pseudintermedius: oxacillin versus cefoxitin. Clinical Microbiology and Infection, 26(1), https://doi.org/10.1016/j.cmi.2019.05.002

Fate of CMY-2-encoding plasmids introduced into the human fecal microbiota by exogenous Escherichia coli (2019)
Journal Article
Anjum, M., Madsen, J. S., Nesme, J., Jana, B., Wiese, M., Jasinskyte, D., …Guardabassi, L. (2019). Fate of CMY-2-encoding plasmids introduced into the human fecal microbiota by exogenous Escherichia coli. Antimicrobial Agents and Chemotherapy, 63(5), https://doi.org/10.1128/AAC.02528-18

The gut is a hot spot for transfer of antibiotic resistance genes from ingested exogenous bacteria to the indigenous microbiota. The objective of this study was to determine the fate of two nearly identical blaCMY-2-harboring plasmids introduced into... Read More about Fate of CMY-2-encoding plasmids introduced into the human fecal microbiota by exogenous Escherichia coli.

Isolation and characterization of bacteriophages active against methicillin-resistant Staphylococcus pseudintermedius (2019)
Journal Article
Moodley, A., Kot, W., Nälgård, S., Jakociune, D., Neve, H., Hansen, L. H., …Vogensen, F. K. (2019). Isolation and characterization of bacteriophages active against methicillin-resistant Staphylococcus pseudintermedius. Research in Veterinary Science, 122, 81-85. https://doi.org/10.1016/j.rvsc.2018.11.008

We aimed to isolate and characterize bacteriophages (phages) with preferential activity against methicillin-resistant Staphylococcus pseudintermedius (MRSP), a multidrug-resistant canine pathogen. Four phages were isolated from canine faeces using tw... Read More about Isolation and characterization of bacteriophages active against methicillin-resistant Staphylococcus pseudintermedius.

Repurposing azithromycin and rifampicin against Gram-negative pathogens by combination with peptide potentiators (2018)
Journal Article
Baker, K. R., Jana, B., Hansen, A. M., Vissing, K. J., Nielsen, H. M., Franzyk, H., & Guardabassi, L. (2018). Repurposing azithromycin and rifampicin against Gram-negative pathogens by combination with peptide potentiators. International Journal of Antimicrobial Agents, 53(6), 868-872. https://doi.org/10.1016/j.ijantimicag.2018.10.025

Gram-negative bacterial pathogens are intrinsically resistant to several antibiotics that are not able to penetrate the cell envelope barrier. The aim of this study was to identify peptides that at low concentrations induce susceptibility to these an... Read More about Repurposing azithromycin and rifampicin against Gram-negative pathogens by combination with peptide potentiators.

Transcriptome analysis of extended-spectrum ß-lactamase-producing Escherichia coli and methicillin-resistant Staphylococcus aureus exposed to cefotaxime (2018)
Journal Article
Brochmann, P. R., Hesketh, A., Jana, B., Brodersen, G. H., & Guardabassi, L. (2018). Transcriptome analysis of extended-spectrum ß-lactamase-producing Escherichia coli and methicillin-resistant Staphylococcus aureus exposed to cefotaxime. https://doi.org/10.1038/s41598-018-34191-3

Previous studies on bacterial response to antibiotics mainly focused on susceptible strains. Here we characterized the transcriptional responses of distinct cephalosporin-resistant bacteria of public health relevance to cefotaxime (CTX), a cephalospo... Read More about Transcriptome analysis of extended-spectrum ß-lactamase-producing Escherichia coli and methicillin-resistant Staphylococcus aureus exposed to cefotaxime.

A culture-independent method for studying transfer of IncI1 plasmids from wild-type Escherichia coli in complex microbial communities (2018)
Journal Article
Anjum, M., Madsen, J. S., Espinosa-Gongora, C., Jana, B., Wiese, M., Nielsen, D. S., …Guardabassi, L. (2018). A culture-independent method for studying transfer of IncI1 plasmids from wild-type Escherichia coli in complex microbial communities. Journal of Microbiological Methods, 152, 18-26. https://doi.org/10.1016/j.mimet.2018.07.009

IncI1 plasmids play a central role in the transfer of antimicrobial resistance genes among Enterobacteriaceae in animals and humans. Knowledge on the dynamics of IncI1 plasmid transfer is limited, mainly due to lack of culture-independent methods tha... Read More about A culture-independent method for studying transfer of IncI1 plasmids from wild-type Escherichia coli in complex microbial communities.

Evidence for the evolutionary steps leading to mecA-mediated ß-lactam resistance in staphylococci (2017)
Journal Article
Rolo, J., Worning, P., Nielsen, J. B., Sobral, R., Bowden, R., Bouchami, O., …Miragaia, M. (2017). Evidence for the evolutionary steps leading to mecA-mediated ß-lactam resistance in staphylococci. PLoS Genetics, 13(4), e1006674. https://doi.org/10.1371/journal.pgen.1006674

The epidemiologically most important mechanism of antibiotic resistance in Staphylococcus aureus is associated with mecA–an acquired gene encoding an extra penicillin-binding protein (PBP2a) with low affinity to virtually all β-lactams. The introduct... Read More about Evidence for the evolutionary steps leading to mecA-mediated ß-lactam resistance in staphylococci.

Cephem Potentiation by Inactivation of Nonessential Genes Involved in Cell Wall Biogenesis of ß-Lactamase-Producing Escherichia coli (2017)
Journal Article
Baker, K. R., Sigurðardóttir, H. H., Jana, B., & Guardabassi, L. (2017). Cephem Potentiation by Inactivation of Nonessential Genes Involved in Cell Wall Biogenesis of ß-Lactamase-Producing Escherichia coli. Antimicrobial Agents and Chemotherapy, 61(3), e01773-16. https://doi.org/10.1128/AAC.01773-16

Reversal of antimicrobial resistance is an appealing and largely unexplored strategy in drug discovery. The objective of this study was to identify potential targets for “helper” drugs reversing cephem resistance in Escherichia coli strains producing... Read More about Cephem Potentiation by Inactivation of Nonessential Genes Involved in Cell Wall Biogenesis of ß-Lactamase-Producing Escherichia coli.

The secondary resistome of multidrug-resistant Klebsiella pneumoniae (2017)
Journal Article
Jana, B., Cain, A. K., Doerrler, W. T., Boinett, C. J., Fookes, M. C., Parkhill, J., & Guardabassi, L. (2017). The secondary resistome of multidrug-resistant Klebsiella pneumoniae. https://doi.org/10.1038/srep42483

Klebsiella pneumoniae causes severe lung and bloodstream infections that are difficult to treat due to multidrug resistance. We hypothesized that antimicrobial resistance can be reversed by targeting chromosomal non-essential genes that are not respo... Read More about The secondary resistome of multidrug-resistant Klebsiella pneumoniae.