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<i>Plasmodium</i> LCCL domain-containing modular proteins have their origins in the ancestral alveolate (2024)
Journal Article
De Hoest-Thompson, C., Marugan-Hernandez, V., & Dessens, J. (2024). Plasmodium LCCL domain-containing modular proteins have their origins in the ancestral alveolate. Open Biology, 14(6), https://doi.org/10.1098/rsob.230451

Plasmodium species encode a unique set of six modular proteins named LCCL lectin domain adhesive-like proteins (LAPs) that operate as a complex and that are essential for malaria parasite transmission from mosquito to vertebrate. LAPs possess complex... Read More about <i>Plasmodium</i> LCCL domain-containing modular proteins have their origins in the ancestral alveolate.

Thyme, Oregano, and Garlic Essential Oils and Their Main Active Compounds Influence <i>Eimeria tenella</i> Intracellular Development (2023)
Journal Article
Felici, M., Tugnoli, B., De Hoest-Thompson, C., Piva, A., Grilli, E., & Marugan-Hernandez, V. (2024). Thyme, Oregano, and Garlic Essential Oils and Their Main Active Compounds Influence Eimeria tenella Intracellular Development. Animals, 14(1), https://doi.org/10.3390/ani14010077

Coccidiosis poses a significant challenge in poultry production and is typically managed with ionophores and chemical anticoccidials. However, the emergence of drug resistance and limitations on their use have encouraged the exploration of alternativ... Read More about Thyme, Oregano, and Garlic Essential Oils and Their Main Active Compounds Influence <i>Eimeria tenella</i> Intracellular Development.

Refractile bodies of Eimeria tenella are proteinaceous membrane-less organelles that undergo dynamic changes during infection (2023)
Journal Article
Burrell, A., Marugan-Hernandez, V., von der Recke, K., Aguiar-Martins, K., Gabriel, H., Tomley, F., & Vaughan, S. (2023). Refractile bodies of Eimeria tenella are proteinaceous membrane-less organelles that undergo dynamic changes during infection. Frontiers in Cellular and Infection Microbiology, 13, https://doi.org/10.3389/fcimb.2023.1082622

IntroductionRefractile bodies (RB) are large membrane-less organelles (MLO) of unknown function found as a prominent mismatched pair within the sporozoite stages of all species of Eimeria, parasitic coccidian protozoa. MethodsHigh resolution imaging... Read More about Refractile bodies of Eimeria tenella are proteinaceous membrane-less organelles that undergo dynamic changes during infection.

Cellular electron tomography of the apical complex in the apicomplexan parasite Eimeria tenella shows a highly organised gateway for regulated secretion. (2022)
Journal Article
Burrell, A., Marugan-Hernandez, V., Moreira-Leite, F., Ferguson, D. J. P., Tomley, F. M., & Vaughan, S. (2022). Cellular electron tomography of the apical complex in the apicomplexan parasite Eimeria tenella shows a highly organised gateway for regulated secretion. Cell, https://doi.org/10.1101/2021.06.17.448283

The apical complex of apicomplexan parasites is essential for host cell invasion and intracellular survival and as the site of regulated exocytosis from specialised secretory organelles called rhoptries and micronemes. Despite its importance, there i... Read More about Cellular electron tomography of the apical complex in the apicomplexan parasite Eimeria tenella shows a highly organised gateway for regulated secretion..

A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens (2022)
Journal Article
Soutter, F., Werling, D., Nolan, M., Küster, T., Attree, E., Marugán-Hernández, V., …Blake, D. (2022). A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens. Frontiers in Immunology, 13, Article 809711. https://doi.org/10.3389/fimmu.2022.809711

Cheap, easy-to-produce oral vaccines are needed for control of coccidiosis in chickens to reduce the impact of this disease on welfare and economic performance. Saccharomyces cerevisiae yeast expressing three Eimeria tenella antigens were developed a... Read More about A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens.

Controlling the causative agents of coccidiosis in domestic chickens; an eye on the 1 past and considerations for the future 2 (2021)
Journal Article
Attree, E., Sanchez-Arsuaga, G., Jones, M., Xia, D., -Hernandez, M., Blake, D., & Tomley, F. (2021). Controlling the causative agents of coccidiosis in domestic chickens; an eye on the 1 past and considerations for the future 2. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, https://doi.org/10.1186/s43170-021-00056-5

Coccidiosis is a potentially severe enteritis caused by species of obligate intracellular parasites of the genus Eimeria. These parasites cause significant economic losses to the poultry industry, predominantly due to compromised efficiency of produc... Read More about Controlling the causative agents of coccidiosis in domestic chickens; an eye on the 1 past and considerations for the future 2.

Do all Coccidia follow the same trafficking rules? (2021)
Journal Article
Marugan-Hernandez, V., Tomley, F., & Sanchez-Arsuaga, G. (2021). Do all Coccidia follow the same trafficking rules?. Life, https://doi.org/10.3390/life11090909

The Coccidia are a subclass of the Apicomplexa including several genera of protozoan parasites that cause important diseases in humans and animals, with Toxoplasma gondii becoming the ‘model organism’ for research into coccidian molecular and cellula... Read More about Do all Coccidia follow the same trafficking rules?.

Spotlight on avian pathology: Eimeria and the disease coccidiosis (2021)
Journal Article
Blake, D. P., Marugan-Hernandez, V., & Tomley, F. M. (in press). Spotlight on avian pathology: Eimeria and the disease coccidiosis. Avian Pathology, https://doi.org/10.1080/03079457.2021.1912288

More than 72 billion chickens were produced globally in 2019, emphasising their major contribution to the production of protein for human consumption. Production systems for chickens are increasingly moving towards drug-free and/or extensive programm... Read More about Spotlight on avian pathology: Eimeria and the disease coccidiosis.

Impact of Eimeria tenella oocyst dose on parasite replication, lesion score and cytokine transcription in the caeca in three breeds of commercial layer chickens (2021)
Journal Article
Soutter, F., Werling, D., Kim, S., Pastor-Fernández, I., Marugan-Hernandez, V., Tomley, F., & Blake, D. (in press). Impact of Eimeria tenella oocyst dose on parasite replication, lesion score and cytokine transcription in the caeca in three breeds of commercial layer chickens. Frontiers in Veterinary Science,

Eimeria species parasites infect the gastrointestinal tract of chickens, causing disease and impacting on production. The poultry industry relies on anticoccidial drugs and live vaccines to control Eimeria and there is a need for novel, scalable alte... Read More about Impact of Eimeria tenella oocyst dose on parasite replication, lesion score and cytokine transcription in the caeca in three breeds of commercial layer chickens.

The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture (2020)
Journal Article
Marugan-Hernandez, V., Jeremiah, G., Aguiar-Martins, K., Burrell, A., Vaughan, S., Xia, D., …Tomley, F. (2020). The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture. Frontiers in Cellular and Infection Microbiology, 10, 579833

In vitro development of the complete life cycle of Eimeria species has been achieved in primary cultures of avian epithelial cells with low efficiency. The use of immortalized cell lines simplifies procedures but only allows partial development throu... Read More about The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture.

In vitro Anticoccidial Study of Oregano and Garlic Essential Oils and Effects on Growth Performance, Fecal Oocyst Output, and Intestinal Microbiota in vivo (2020)
Journal Article
Sidiropoulou, E., Skoufos, I., Marugan-Hernandez, V., Giannenas, I., Bonos, E., Aguiar-Martins, K., …Tzora, A. (2020). In vitro Anticoccidial Study of Oregano and Garlic Essential Oils and Effects on Growth Performance, Fecal Oocyst Output, and Intestinal Microbiota in vivo. Frontiers in Veterinary Science, 7, https://doi.org/10.3389/fvets.2020.00420

This study investigated the in vitro effects of Greek oregano and garlic essential oils on inhibition of Eimeria parasites and their in vivo effects on production performance, intestinal bacteria counts, and oocyst output. An inhibition assay was per... Read More about In vitro Anticoccidial Study of Oregano and Garlic Essential Oils and Effects on Growth Performance, Fecal Oocyst Output, and Intestinal Microbiota in vivo.

Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high level E. maxima challenge in a broiler model of coccidiosis. (2020)
Journal Article
Pastor-Fernández, I., Sungwon, K., Marugán-Hernández, V., Soutter, F., Tomley, F., & Blake, D. (2020). Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high level E. maxima challenge in a broiler model of coccidiosis. Parasites and Vectors,

Poultry coccidiosis is a parasitic enteric disease with a highly negative impact on chicken production. In-feed chemoprophylaxis remains the primary method of control, but the increasing ineffectiveness of anticoccidial drugs, and potential future re... Read More about Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high level E. maxima challenge in a broiler model of coccidiosis..

Life cycle stages, specific organelles and invasion mechanisms of Eimeria species (2019)
Journal Article
Burrell, A., Tomley, F. M., Vaughan, S., & Marugan-Hernandez, V. (2019). Life cycle stages, specific organelles and invasion mechanisms of Eimeria species. Parasitology, https://doi.org/10.1017/S0031182019001562

Apicomplexans, including species of Eimeria, pose a real threat to the health and wellbeing of animals and humans. Eimeria parasites do not infect humans but cause an important economic impact on livestock, in particular on the poultry industry. Desp... Read More about Life cycle stages, specific organelles and invasion mechanisms of Eimeria species.

Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens (2018)
Journal Article
Pastor-Fernández, I., Kim, S., Billington, K., Bumstead, J., Marugán-Hernández, V., Küster, T., …Tomley, F. M. (2018). Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens. International Journal for Parasitology, 48(7), 505-518. https://doi.org/10.1016/j.ijpara.2018.01.003

Recently, the availability of protocols supporting genetic complementation of Eimeria has raised the prospect of generating transgenic parasite lines which can function as vaccine vectors, expressing and delivering heterologous proteins. Complementat... Read More about Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens.

Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella (2017)
Journal Article
Marugan-Hernandez, V., Fiddy, R., Nurse-Francis, J., Smith, O., Pritchard, L., & Tomley, F. M. (2017). Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella. https://doi.org/10.1186/s13071-017-2454-4

In this study, we evaluated the binding capacity of Type I MAR domains from novel E. tenella MCPs. Variants of the previously described HxT motif were analysed showing that HxT and VxT variants bind, whereas HxS and YxE variants did not. One of these... Read More about Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella.

Neospora caninum and Bovine Neosporosis: Current Vaccine Research (2017)
Journal Article
Marugan-Hernandez, V. (2017). Neospora caninum and Bovine Neosporosis: Current Vaccine Research. Journal of Comparative Pathology, 157(2-3), 193-200. https://doi.org/10.1016/j.jcpa.2017.08.001

Neospora caninum, a tissue cyst-forming parasite, is the causative agent of bovine neosporosis. It is considered to be one of the most important transmissible causes of reproductive failure in cattle; abortion and neonatal mortality result in signifi... Read More about Neospora caninum and Bovine Neosporosis: Current Vaccine Research.

Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle (2017)
Journal Article
Marugan-Hernandez, V., Long, E., Blake, D. P., Crouch, C., & Tomley, F. M. (2017). Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle. https://doi.org/10.1038/s41598-017-04049-1

Eimeria spp. are intracellular parasites that have a major impact on poultry. Effective live vaccines are available and the development of reverse genetic technologies has raised the prospect of using Eimeria spp. as recombinant vectors to express ad... Read More about Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle.

The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress (2016)
Journal Article
Pastor-Fernandez, I., Regidor-Cerrillo, J., Alvarez-Garcia, G., Marugan-Hernandez, V., Garcia-Lunar, P., Hemphill, A., & Ortega-Mora, L. M. (2016). The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress. https://doi.org/10.1186/s13071-016-1620-4