Challenges in defining the functional, non‐coding, expressed genome of members of the Mycobacterium tuberculosis complex (2021)
Journal Article
Stiens, J., Arnvig, K. B., Kendall, S. L., & Nobeli, I. (2022). Challenges in defining the functional, non‐coding, expressed genome of members of the Mycobacterium tuberculosis complex. Molecular Microbiology, 117(1), 20-31. https://doi.org/10.1111/mmi.14862

A definitive transcriptome atlas for the non-coding expressed elements of the members of the Mycobacterium tuberculosis complex (MTBC) does not exist. Incomplete lists of non-coding transcripts can be obtained for some of the reference genomes (e.g.,... Read More about Challenges in defining the functional, non‐coding, expressed genome of members of the Mycobacterium tuberculosis complex.

Probing differences in gene essentiality between the human and animal adapted (2021)
Journal Article
Gibson, A. J., Passmore, I. J., Faulkner, V., Xia, D., Nobeli, I., Stiens, J., …Kendall, S. L. (2021). Probing differences in gene essentiality between the human and animal adapted. Frontiers in Veterinary Science,

Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences and phenotypes despite being >99% identical at the nucleic acid level. Previous studies have explored gene expression changes between the members, ho... Read More about Probing differences in gene essentiality between the human and animal adapted.

Population Pharmacokinetics of Intravenous Amoxicillin Combined With Clavulanic Acid in Healthy and Critically Ill Dogs (2021)
Journal Article
Vegas Cómitre, M., Cortellini, S., Devreese, M., Roques, B., Bousquet-Melou, A., Toutain, P., & Pelligand, L. (2021). Population Pharmacokinetics of Intravenous Amoxicillin Combined With Clavulanic Acid in Healthy and Critically Ill Dogs. Journal of Veterinary Medicine, https://doi.org/10.3389/fvets.2021.770202

Background – Data regarding antimicrobial pharmacokinetics (PK) in critically ill dogs is lacking and likely differs from healthy dogs. Hypothesis/Objectives —To describe a population PK model for intravenous amoxicillin clavulanic acid (AMC) in... Read More about Population Pharmacokinetics of Intravenous Amoxicillin Combined With Clavulanic Acid in Healthy and Critically Ill Dogs.

Molecular basis for DarT ADP-ribosylation of a DNA base (2021)
Journal Article
Schuller, M., Butler, R., Ariza, A., Tromans-Coia, C., Jankevicius, G., Claridge, T., …Ahel, I. (2021). Molecular basis for DarT ADP-ribosylation of a DNA base. Nature, https://doi.org/10.1038/s41586-021-03825-4

ADP-ribosyltransferases (ARTs) utilise NAD+ to catalyse substrate ADP-ribosylation, thereby regulating cellular pathways or contributing to toxin-mediated pathogenicity of bacteria. Reversible ADP-ribosylation has traditionally been considered a prot... Read More about Molecular basis for DarT ADP-ribosylation of a DNA base.

Assessment of evaluation tools for integrated surveillance of antimicrobial use and resistance based on selected case studies (2021)
Journal Article
Bennani, H., Sandberg, M., Hesp, A., Aenishaenslin, C., Bordier, M., Bergwerff, U., …Alban, L. (2021). Assessment of evaluation tools for integrated surveillance of antimicrobial use and resistance based on selected case studies. Frontiers in Veterinary Science, https://doi.org/10.3389/fvets.2021.620998

Regular evaluation of integrated surveillance for antimicrobial use (AMU) and resistance (AMR) in animals, humans and the environment is needed to ensure system effectiveness, but the question is how. In this study, six different evaluation tools wer... Read More about Assessment of evaluation tools for integrated surveillance of antimicrobial use and resistance based on selected case studies.

AMHU Surveillance GB Beef Cattle And Sheep Research Brief 2021 (2021)
Report
Arnold, J., Whatford, L., Payne-Gifford, S., Tak, M., Van Winden, S., Barling, D., & Hӓsler, B. (2021). AMHU Surveillance GB Beef Cattle And Sheep Research Brief 2021. RVC Research Online: Cadogan Trust

Antimicrobial and anthelmintic usage (AMHU) in livestock production has increased over the last 50+ years. Microbial and helminthic exposure to such drugs is understood to have contributed to increased resistance to treatment, causing significant eco... Read More about AMHU Surveillance GB Beef Cattle And Sheep Research Brief 2021.

Systems-thinking approach to identify and assess feasibility of potential interventions to reduce antibiotic use in tilapia farming in Egypt (2021)
Journal Article
Desbois, A., Garza, M., Eltholth, M., Hegazy, Y., Mateus, A., Adams, A., …Brunton, L. (in press). Systems-thinking approach to identify and assess feasibility of potential interventions to reduce antibiotic use in tilapia farming in Egypt. Aquaculture,

Re-sensitization of Mycobacterium smegmatis to Rifampicin Using CRISPR Interference Demonstrates Its Utility for the Study of Non-essential Drug Resistance Traits (2021)
Journal Article
Kendall, S. L., Faulkner, V., Cox, A. A., Goh, S., van Bohemen, A., Gibson, A. J., …Willcocks, S. (in press). Re-sensitization of Mycobacterium smegmatis to Rifampicin Using CRISPR Interference Demonstrates Its Utility for the Study of Non-essential Drug Resistance Traits. Frontiers in Microbiology, 11, https://doi.org/10.3389/fmicb.2020.619427

A greater understanding of the genes involved in antibiotic resistance in Mycobacterium tuberculosis (Mtb) is necessary for the design of improved therapies. Clustered regularly interspaced short palindromic repeat interference (CRISPRi) has been pre... Read More about Re-sensitization of Mycobacterium smegmatis to Rifampicin Using CRISPR Interference Demonstrates Its Utility for the Study of Non-essential Drug Resistance Traits.