Fabrice Lucien
MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research
Lucien, Fabrice; Gustafson, Dakota; Lenassi, Metka; Li, Bo; Teske, Jacob J.; Boilard, Eric; von Hohenberg, Katharina Clemm; Falcón‐Perez, Juan Manual; Gualerzi, Alice; Reale, Antonia; Jones, Jennifer C.; Lässer, Cecilia; Lawson, Charlotte; Nazarenko, Irina; O'Driscoll, Lorraine; Pink, Ryan; Siljander, Pia R‐M; Soekmadji, Carolina; Hendrix, An; Welsh, Joshua A; Witwer, Kenneth W.; Nieuwland, Rienk
Authors
Dakota Gustafson
Metka Lenassi
Bo Li
Jacob J. Teske
Eric Boilard
Katharina Clemm von Hohenberg
Juan Manual Falcón‐Perez
Alice Gualerzi
Antonia Reale
Jennifer C. Jones
Cecilia Lässer
Charlotte Lawson
Irina Nazarenko
Lorraine O'Driscoll
Ryan Pink
Pia R‐M Siljander
Carolina Soekmadji
An Hendrix
Joshua A Welsh
Kenneth W. Witwer
Rienk Nieuwland
Abstract
Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor-dependent but also influenced by collection and preparation protocols. Since there are hundreds of pre-analytical protocols and over forty variables, the development of standard operating procedures for EV research is very challenging. To improve the reproducibility of blood EV research, the International Society for Extracellular Vesicles (ISEV) Blood EV Task Force proposes standardized reporting of (i) the applied blood collection and preparation protocol and (ii) the quality of the prepared plasma and serum samples. Gathering detailed information will provide insight into the performance of the protocols and more effectively identify potential confounders in the prepared plasma and serum samples. To collect this information, the ISEV Blood EV Task Force created the Minimal Information for Blood EV research (MIBlood-EV), a tool to record and report information about pre-analytical protocols used for plasma and serum preparation as well as assays used to assess the quality of these preparations. This tool does not require modifications of established local pre-analytical protocols and can be easily implemented to enhance existing databases thereby enabling evidence-based optimization of pre-analytical protocols through meta-analysis. Taken together, insight into the quality of prepared plasma and serum samples will (i) improve the quality of biobanks for EV research, (ii) guide the exchange of plasma and serum samples between biobanks and laboratories, (iii) facilitate inter-laboratory comparative EV studies, and (iv) improve the peer review process.
Keywords: biomarker; blood; extracellular vesicles; liquid biopsy; quality control; reproducibility; standardization.
Citation
Lucien, F., Gustafson, D., Lenassi, M., Li, B., Teske, J. J., Boilard, E., …Nieuwland, R. (2023). MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research. Journal of Extracellular Vesicles, 12(12), https://doi.org/10.1002/jev2.12385
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 10, 2023 |
Online Publication Date | Dec 8, 2023 |
Publication Date | 2023-12 |
Deposit Date | Jan 5, 2024 |
Publicly Available Date | Jan 10, 2024 |
Journal | Journal of Extracellular Vesicles |
Electronic ISSN | 2001-3078 |
Publisher | Taylor & Francis Open Access |
Peer Reviewed | Peer Reviewed |
Volume | 12 |
Issue | 12 |
DOI | https://doi.org/10.1002/jev2.12385 |
Keywords | Cell Biology; Histology |
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MIBlood‐EV: Minimal Information To Enhance The Quality And Reproducibility Of Blood Extracellular Vesicle Research
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Licence
http://creativecommons.org/licenses/by-nc/4.0/
Publisher Licence URL
http://creativecommons.org/licenses/by-nc/4.0/
Version
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