The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation
(2014)
Journal Article
Bossé, J. T., Soares-Bazzolli, D. M., Li, Y., Wren, B. W., Tucker, A. W., Maskell, D. J., …BRaDP1T Consortium, . (2014). The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation. PLoS ONE, 9(11), https://doi.org/10.1371/journal.pone.0111252
We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinase... Read More about The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation.