All Outputs (4)

Genetic disruption of Plasmodium falciparum Merozoite surface antigen 180 (PfMSA180) suggests an essential role during parasite egress from erythrocytes (2021)
Journal Article
Bahl, V., Chaddha, K., Mian, S., Holder, A., Knuepfer, E., & Gaur, D. (2021). Genetic disruption of Plasmodium falciparum Merozoite surface antigen 180 (PfMSA180) suggests an essential role during parasite egress from erythrocytes. Scientific Reports, 11(1), https://doi.org/10.1038/s41598-021-98707-0

Plasmodium falciparum, the parasite responsible for severe malaria, develops within erythrocytes. Merozoite invasion and subsequent egress of intraerythrocytic parasites are essential for this erythrocytic cycle, parasite survival and pathogenesis. I... Read More about Genetic disruption of Plasmodium falciparum Merozoite surface antigen 180 (PfMSA180) suggests an essential role during parasite egress from erythrocytes.

Autocatalytic activation of a malarial egress protease is druggable and requires a protein cofactor (2021)
Journal Article
Tan, M., Koussis, K., Withers-Martinez, C., Howell, S., Thomas, J., Hackett, F., …Blackman, M. (2021). Autocatalytic activation of a malarial egress protease is druggable and requires a protein cofactor. EMBO Journal, 40(11), https://doi.org/10.15252/embj.2020107226

Malaria parasite egress from host erythrocytes (RBCs) is regulated by discharge of a parasite serine protease called SUB1 into the parasitophorous vacuole (PV). There, SUB1 activates a PV-resident cysteine protease called SERA6, enabling host RBC rup... Read More about Autocatalytic activation of a malarial egress protease is druggable and requires a protein cofactor.

Inhibition of protein N-myristoylation blocks Plasmodium falciparum intraerythrocytic development, egress and invasion (2021)
Journal Article
Schlott, A., Knuepfer, E., Green, J., Hobson, P., Borg, A., Morales-Sanfrutos, J., …Holder, A. (2021). Inhibition of protein N-myristoylation blocks Plasmodium falciparum intraerythrocytic development, egress and invasion. PLoS Biology, 19(10), https://doi.org/10.1371/journal.pbio.3001408

We have combined chemical biology and genetic modification approaches to investigate the importance of protein myristoylation in the human malaria parasite, Plasmodium falciparum. Parasite treatment during schizogony in the last 10 to 15 hours of the... Read More about Inhibition of protein N-myristoylation blocks Plasmodium falciparum intraerythrocytic development, egress and invasion.

Deletion of Plasmodium falciparum ubc13 increases parasite sensitivity to the mutagen, methyl methanesulfonate and dihydroartemisinin (2021)
Journal Article
Maneekesorn, S., Knuepfer, E., Green, J. L., Prommana, P., Uthaipibull, C., Srichairatanakool, S., & Holder, A. A. (in press). Deletion of Plasmodium falciparum ubc13 increases parasite sensitivity to the mutagen, methyl methanesulfonate and dihydroartemisinin. Scientific Reports, 11(1), Article 21791. https://doi.org/10.1038/s41598-021-01267-6

The inducible Di-Cre system was used to delete the putative ubiquitin-conjugating enzyme 13 gene (ubc13) of Plasmodium falciparum to study its role in ubiquitylation and the functional consequence during the parasite asexual blood stage. Deletion res... Read More about Deletion of Plasmodium falciparum ubc13 increases parasite sensitivity to the mutagen, methyl methanesulfonate and dihydroartemisinin.