Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing

Javaheri, B; Carriero, A; Staines, K A; Chang, Y M; Houston, D A; Oldknow, K J; Millan, J L; Kazeruni, B N; Salmon, P; Shefelbine, S; Farquharson, C; Pitsillides, A A

doi:10.1016/j.bone.2015.07.035

A distinctive patchy osteomalacia characterises Phospho1-deficient mice (2017)
Journal Article
Boyde, A., Staines, K. A., Javaheri, B., Millan, J. L., Pitsillides, A. A., & Farquharson, C. (2017). A distinctive patchy osteomalacia characterises Phospho1-deficient mice. Journal of Anatomy, 231(2), 293-308. https://doi.org/10.1111/joa.12628

The phosphatase PHOSPHO1 is involved in the initiation of biomineralisation. Bones in Phospho1 knockout (KO) mice show histological osteomalacia with frequent bowing of long bones and spontaneous fractures: they contain less mineral, with smaller min... Read More about A distinctive patchy osteomalacia characterises Phospho1-deficient mice.

Finite-element modelling of mechanobiological factors influencing sesamoid tissue morphology in the patellar tendon of an ostrich (2017)
Journal Article
Chadwick, K., Shefelbine, S. J., Pitsillides, A. A., & Hutchinson, J. R. (2017). Finite-element modelling of mechanobiological factors influencing sesamoid tissue morphology in the patellar tendon of an ostrich. Royal Society Open Science, 4(6), 170133. https://doi.org/10.1098/rsos.170133

The appearance and shape of sesamoid bones within a tendon or ligament wrapping around a joint are understood to be influenced by both genetic and epigenetic factors. Ostriches (Struthio camelus) possess two sesamoid patellae (kneecaps), one of which... Read More about Finite-element modelling of mechanobiological factors influencing sesamoid tissue morphology in the patellar tendon of an ostrich.

The STR/ort mouse model of spontaneous osteoarthritis – an update (2017)
Journal Article
Staines, K. A., Poulet, B., Wentworth, D. N., & Pitsillides, A. A. (2017). The STR/ort mouse model of spontaneous osteoarthritis – an update. Osteoarthritis and Cartilage, 25(6), 802-808. https://doi.org/10.1016/j.joca.2016.12.014

Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation (2017)
Journal Article
Staines, K. A., Javaheri, B., Hohenstein, P., Fleming, R., Ikpegbu, E., Unger, E., …Farquharson, C. (2017). Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation. Journal of Cellular Physiology, 232(11), 3006-3019. https://doi.org/10.1002/jcp.25999

The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mechanisms are still unknown. Due to the known embryonic lethality induced by gl... Read More about Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation.

Detection of early osteogenic commitment in primary cells using Raman spectroscopy (2017)
Journal Article
Smith, S. J., Emery, R., Pitsillides, A. A., Clarkin, C. E., & Mahajan, S. (2017). Detection of early osteogenic commitment in primary cells using Raman spectroscopy. Analyst, 142(11), 1962-1973. https://doi.org/10.1039/C6AN02469F

Major challenges in the development of novel implant surfaces for artificial joints include osteoblast heterogeneity and the lack of a simple and sensitive in vitro assay to measure early osteogenic responses. Raman spectroscopy is a label-free, non-... Read More about Detection of early osteogenic commitment in primary cells using Raman spectroscopy.

Altered Bone Mechanics, Architecture and Composition in the Skeleton of TIMP-3-Deficient Mice (2017)
Journal Article
Miller, B., Spevak, L., Lukashova, L., Javaheri, B., Pitsillides, A. A., Boskey, A., …Carriero, A. (2017). Altered Bone Mechanics, Architecture and Composition in the Skeleton of TIMP-3-Deficient Mice. Calcified Tissue International, 100(6), 631–640. https://doi.org/10.1007/s00223-017-0248-5

The role of embryo movement in the development of the furcula (2017)
Journal Article
Pollard, A. S., Boyd, S., McGonnell, I. M., & Pitsillides, A. A. (2017). The role of embryo movement in the development of the furcula. Journal of Anatomy, 230(3), 435-443. https://doi.org/10.1111/joa.12571

The pectoral girdle is a complex structure which varies in its morphology between species. A major component in birds is the furcula, which can be considered equivalent to a fusion of the paired clavicles found in many mammals, and the single intercl... Read More about The role of embryo movement in the development of the furcula.

Limb proportions show developmental plasticity in response to embryo movement (2017)
Journal Article
Pollard, A. S., Charlton, B. G., Hutchinson, J. R., Gustafsson, T., McGonnell, I. M., Timmons, J. A., & Pitsillides, A. A. (2017). Limb proportions show developmental plasticity in response to embryo movement. https://doi.org/10.1038/srep41926

Animals have evolved limb proportions adapted to different environments, but it is not yet clear to what extent these proportions are directly influenced by the environment during prenatal development. The developing skeleton experiences mechanical l... Read More about Limb proportions show developmental plasticity in response to embryo movement.

Pathogenic LRRK2 variants are gain-of-function mutations that enhance LRRK2-mediated repression of beta-catenin signaling (2017)
Journal Article
Berwick, D. C., Javaheri, B., Wetzel, A., Hopkinson, M., Nixon-Abell, J., Granno, S., …Harvey, K. (2017). Pathogenic LRRK2 variants are gain-of-function mutations that enhance LRRK2-mediated repression of beta-catenin signaling. Molecular Neurodegeneration, 12(9), https://doi.org/10.1186/s13024-017-0153-4

Strain uses gap junctions to reverse stimulation of osteoblast proliferation by osteocytes (2017)
Journal Article
Suswillo, R. F. L., Javaheri, B., Rawlinson, S. C. F., Dowthwaite, G. P., Lanyon, L. E., & Pitsillides, A. A. (2017). Strain uses gap junctions to reverse stimulation of osteoblast proliferation by osteocytes. Cell Biochemistry and Function, 35(1), 56-65. https://doi.org/10.1002/cbf.3245

Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues (2017)
Journal Article
Al-Jazzar, A., Javaheri, B., Prideaux, M., Boyde, A., Scudamore, C. L., Cherifi, C., …Pitsillides, A. A. (2017). Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues. International Journal of Molecular Sciences, 18(1), https://doi.org/10.3390/ijms18010029

Mice harbouring a dentin matrix protein 1 (Dmp1) promoter-driven human diphtheria toxin (DT) receptor (HDTR) transgene (Tg) have recently been used to attain targeted ablation of osteocytes by diphtheria toxin (DT) treatment in order to define osteoc... Read More about Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues.

Overexpression of TIMP-3 in chondrocytes produces transient reduction in growth plate length but permanently reduces adult bone quality and quantity (2016)
Journal Article
Poulet, B., Liu, K., Plumb, D., Vo, P., Shah, M., Staines, K. A., …Bou-Gharios, G. (2016). Overexpression of TIMP-3 in chondrocytes produces transient reduction in growth plate length but permanently reduces adult bone quality and quantity. PLoS ONE, 11(12), e0167971. https://doi.org/10.1371/journal.pone.0167971

E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models (2016)
Journal Article
Staines, K. A., Prideaux, M., Allen, S. P., Buttle, D. J., Pitsillides, A. A., & Farquharson, C. (2016). E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models. Journal of Cellular Physiology, 231(6), 1392-1404. https://doi.org/10.1002/jcp.25282

The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are still unknown. Using the late osteoblast MLO-A5 cell line we reveal increased E... Read More about E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models.

Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo (2016)
Journal Article
Pollard, A. S., Pitsillides, A. A., & Portugal, S. J. (2016). Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo. Physiological and Biochemical Zoology, 89(4), https://doi.org/10.1086/687228

Anatomy, morphology and evolution of the patella in squamate lizards and tuatara (Sphenodon punctatus) (2016)
Journal Article
Regnault, S., Jones, M. E. H., Pitsillides, A. A., & Hutchinson, J. R. (2016). Anatomy, morphology and evolution of the patella in squamate lizards and tuatara (Sphenodon punctatus). Journal of Anatomy, 228, 864-876. https://doi.org/10.1111/joa.12435

Endochondral growth defect and deployment of transient chondrocyte behaviours underlie osteoarthritis onset in a natural murine model (2016)
Journal Article
Staines, K. A., Madi, K., Mirczuk, S. M., Parker, S., Burleigh, A., Poulet, B., …Pitsillides, A. A. (2016). Endochondral growth defect and deployment of transient chondrocyte behaviours underlie osteoarthritis onset in a natural murine model. https://doi.org/10.1002/art.39508

Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing (2016)
Journal Article
Zaman, G., Staines, K. A., Farquharson, C., Newton, P. T., Dudhia, J., Chenu, C., …Dhoot, G. K. (2016). Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing. Histochemistry and Cell Biology, 145(1), 67-79. https://doi.org/10.1007/s00418-015-1365-8

A homeostatic function of CXCR2 signalling in articular cartilage (2015)
Journal Article
Sherwood, J., Bertrand, J., Nalesso, G., Poulet, B., Pitsillides, A. A., Brandolini, L., …Dell'Accio, F. (2015). A homeostatic function of CXCR2 signalling in articular cartilage. Annals of the Rheumatic Diseases, 74(12), 2207-2215. https://doi.org/10.1136/annrheumdis-2014-205546

Predicting cortical bone adaptation to axial loading in the mouse tibia (2015)
Journal Article
Pereira, A. F., Javaheri, B., Pitsillides, A. A., & Shefelbine, S. J. (2015). Predicting cortical bone adaptation to axial loading in the mouse tibia. https://doi.org/10.1098/rsif.2015.0590

The development of predictive mathematical models can contribute to a deeper understanding of the specific stages of bone mechanobiology and the process by which bone adapts to mechanical forces. The objective of this work was to predict, with spatia... Read More about Predicting cortical bone adaptation to axial loading in the mouse tibia.

Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing (2015)
Journal Article
Javaheri, B., Carriero, A., Staines, K. A., Chang, Y. M., Houston, D. A., Oldknow, K. J., …Pitsillides, A. A. (2015). Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing. BONE, 81, 277-291. https://doi.org/10.1016/j.bone.2015.07.035

PHOSPHO1 is one of principal proteins involved in initiating bone matrix mineralisation. Recent studies have found that Phospho1 KO mice (Phospho1-R74X) display multiple skeletal abnormalities with spontaneous fractures, bowed long bones, osteomalaci... Read More about Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing.

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