All Outputs (3)

The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals (2019)
Journal Article
Swift, B. M. C., Meade, N., Barron, E. S., Bennett, M., Perehenic, T., Hughes, V., Stevenson, K., & Rees, C. E. D. (2019). The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals. Microbial Biotechnology, 13(3), 738-746. https://doi.org/10.1111/1751-7915.13518

Here, we describe the development of a method that exploits bacteriophage D29 as a lysis agent for efficient DNA extraction from low numbers of mycobacterial cells. This method (Actiphage®) used in combination with PCR achieved rapid and sensitive (L... Read More about The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals.

The specificity of phage testing for MAP — where might it fit into the diagnostic armoury? (2019)
Journal Article
Swift, B. M. C., & Rees, C. E. D. (2019). The specificity of phage testing for MAP — where might it fit into the diagnostic armoury?. Livestock, 24(4), https://doi.org/10.12968/live.2019.24.4.176

The current individual tools available for the diagnosis of Johne's disease are far from suitable to tackle this endemic disease. Culture, polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests, when used together can be... Read More about The specificity of phage testing for MAP — where might it fit into the diagnostic armoury?.

A novel, high-sensitivity, bacteriophage-based assay identifies low level Mycobacterium tuberculosis bacteraemia in immunocompetent patients with active and incipient tuberculosis (2019)
Journal Article
Verma, R., Swift, B. M. C., Handley-Hartill, W., Lee, J. K., Woltmann, G., Rees, C. E. D., & Haldar, P. (2019). A novel, high-sensitivity, bacteriophage-based assay identifies low level Mycobacterium tuberculosis bacteraemia in immunocompetent patients with active and incipient tuberculosis. Clinical Infectious Diseases, 70(5), 933-936. https://doi.org/10.1093/cid/ciz548

Haematogenous dissemination of M. tuberculosis (Mtb) is critical to pathogenesis of progressive tuberculous infection in animal models. Using a novel phage-based blood assay, we report the first concordant evidence in well-characterised immunocompete... Read More about A novel, high-sensitivity, bacteriophage-based assay identifies low level Mycobacterium tuberculosis bacteraemia in immunocompetent patients with active and incipient tuberculosis.