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All Outputs (15)

Postnatal mechanical loading drives adaptation of tissues primarily through modulation of the non-collagenous matrix (2020)
Journal Article
Zamboulis, D. E., Thorpe, C. T., Ashraf Kharaz, Y., Birch, H. L., Screen, H. R. C., & Clegg, P. D. (2020). Postnatal mechanical loading drives adaptation of tissues primarily through modulation of the non-collagenous matrix. eLife, 9,

Mature connective tissues demonstrate highly specialised properties, remarkably adapted to meet their functional requirements. Tissue adaptation to environmental cues can occur throughout life and poor adaptation commonly results in injury. However,... Read More about Postnatal mechanical loading drives adaptation of tissues primarily through modulation of the non-collagenous matrix.

Bimodal Whole-Mount Imaging of Tendon Using Confocal Microscopy and X-ray Micro-Computed Tomography (2020)
Journal Article
Marr, N., Hopkinson, M., Hibbert, A., Pitsillides, A., & Thorpe, C. (2020). Bimodal Whole-Mount Imaging of Tendon Using Confocal Microscopy and X-ray Micro-Computed Tomography. Biological Procedures Online, https://doi.org/10.1186/s12575-020-00126-4

BACKGROUND:3-dimensional imaging modalities for optically dense connective tissues such as tendons are limited and typically have a single imaging methodological endpoint. Here, we have developed a bimodal procedure utilising fluorescence-based confo... Read More about Bimodal Whole-Mount Imaging of Tendon Using Confocal Microscopy and X-ray Micro-Computed Tomography.

Heterogeneity of proteome dynamics between connective tissue phases of adult tendon (2020)
Journal Article
Choi, H., Simpson, D., Wang, D., Prescott, M., Pitsillides, A. A., Dudhia, J., Clegg, P. D., Ping, P., & Thorpe, C. T. (2020). Heterogeneity of proteome dynamics between connective tissue phases of adult tendon. eLife, 9, https://doi.org/10.7554/elife.55262

Maintenance of connective tissue integrity is fundamental to sustain function, requiring protein turnover to repair damaged tissue. However, connective tissue proteome dynamics remain largely undefined, as do differences in turnover rates of individu... Read More about Heterogeneity of proteome dynamics between connective tissue phases of adult tendon.

Structure and collagen crimp patterns of functionally distinct equine tendons, revealed by quantitative polarised light microscopy (qPLM) (2018)
Journal Article
Spiesz, E. M., Thorpe, C. T., Thurner, P. J., & Screen, H. R. C. (2018). Structure and collagen crimp patterns of functionally distinct equine tendons, revealed by quantitative polarised light microscopy (qPLM). Acta Biomaterialia, 70, 281-292. https://doi.org/10.1016/j.actbio.2018.01.034

Structure-function relationships in tendons are directly influenced by the arrangement of collagen fibres. However, the details of such arrangements in functionally distinct tendons remain obscure. This study demonstrates the use of quantitative pola... Read More about Structure and collagen crimp patterns of functionally distinct equine tendons, revealed by quantitative polarised light microscopy (qPLM).

Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing (2017)
Journal Article
Godinho, M. S., Thorpe, C. T., Greenwald, S. E., & Screen, H. R. (2017). Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing. https://doi.org/10.1038/s41598-017-09995-4

Tendon is composed of fascicles bound together by the interfascicular matrix (IFM). Energy storing tendons are more elastic and extensible than positional tendons; behaviour provided by specialisation of the IFM to enable repeated interfascicular sli... Read More about Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing.

Distribution of proteins within different compartments of tendon varies according to tendon type (2016)
Journal Article
Thorpe, C. T., Karunaseelan, K. J., Ng Chieng Hin, J., Riley, G. P., Birch, H. L., Clegg, P. D., & Screen, H. R. (2016). Distribution of proteins within different compartments of tendon varies according to tendon type. Journal of Anatomy, 229(3), 450-8. https://doi.org/10.1111/joa.12485

Although the predominant function of all tendons is to transfer force from muscle to bone and position the limbs, some tendons additionally function as energy stores, reducing the energetic cost of locomotion. To maximise energy storage and return, e... Read More about Distribution of proteins within different compartments of tendon varies according to tendon type.

Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition (2016)
Journal Article
Thorpe, C. T., Peffers, M. J., Simpson, D., Hallwell, E., Screen, H. R., & Clegg, P. D. (2016). Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition. https://doi.org/10.1038/srep20455

Tendon is a simple aligned fibre composite, consisting of collagen-rich fascicles surrounded by a softer interfascicular matrix (IFM). The composition and interactions between these material phases are fundamental in ensuring tissue mechanics meet fu... Read More about Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition.

Fascicles and the interfascicular matrix show adaptation for fatigue resistance in energy storing tendons (2016)
Journal Article
Thorpe, C. T., Riley, G. P., Birch, H. L., Clegg, P. D., & Screen, H. R. C. (2016). Fascicles and the interfascicular matrix show adaptation for fatigue resistance in energy storing tendons. Acta Biomaterialia, 42, 308-315. https://doi.org/10.1016/j.actbio.2016.06.012

Tendon is composed of rope-like fascicles, bound together by interfascicular matrix (IFM). Our previous work shows that the IFM is critical for tendon function, facilitating sliding between fascicles to allow tendons to stretch. This function is part... Read More about Fascicles and the interfascicular matrix show adaptation for fatigue resistance in energy storing tendons.

The interfascicular matrix enables fascicle sliding and recovery in tendon, and behaves more elastically in energy storing tendons (2015)
Journal Article
Thorpe, C. T., Godinho, M. S. C., Riley, G. P., Birch, H. L., Clegg, P. D., & Screen, H. R. C. (2015). The interfascicular matrix enables fascicle sliding and recovery in tendon, and behaves more elastically in energy storing tendons. Journal of the Mechanical Behavior of Biomedical Materials, 52, 85-94. https://doi.org/10.1016/j.jmbbm.2015.04.009

Tendon overload results in alterations in cell shape and increased markers of inflammation and matrix degradation (2015)
Journal Article
Thorpe, C. T., Chaudhry, S., Lei, I., Varone, A., Riley, G. P., Birch, H. L., Clegg, P. D., & Screen, H. R. (2015). Tendon overload results in alterations in cell shape and increased markers of inflammation and matrix degradation. https://doi.org/10.1111/sms.12333

Tendon injury is thought to involve both damage accumulation within the matrix and an accompanying cell response. While several studies have characterized cell and matrix response in chronically injured tendons, few have assessed the initial response... Read More about Tendon overload results in alterations in cell shape and increased markers of inflammation and matrix degradation.

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise (2015)
Journal Article
Spiesz, E. M., Thorpe, C. T., Chaudhry, S., Riley, G. P., Birch, H. L., Clegg, P. D., & Screen, H. R. (2015). Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise. Journal of Orthopaedic Research, 33(6), 889-97. https://doi.org/10.1002/jor.22879

The role of inflammation in tendon injury is uncertain and a topic of current interest. In vitro studies of tendon accelerated overload damage can serve as a valuable source of information on the early stages of tendinopathy. Viable fascicle bundles... Read More about Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise.

Proteomic Analysis Reveals Age-related Changes in Tendon Matrix Composition, with Age- and Injury-specific Matrix Fragmentation (2014)
Journal Article
Peffers, M. J., Thorpe, C. T., Collins, J. A., Eong, R., Wei, T. K., Screen, H. R., & Clegg, P. D. (2014). Proteomic Analysis Reveals Age-related Changes in Tendon Matrix Composition, with Age- and Injury-specific Matrix Fragmentation. Journal of Biological Chemistry, 289(37), 25867-78. https://doi.org/10.1074/jbc.M114.566554

Energy storing tendons, such as the human Achilles and equine superficial digital flexor tendon (SDFT), are highly prone to injury, the incidence of which increases with aging. The cellular and molecular mechanisms that result in increased injury in... Read More about Proteomic Analysis Reveals Age-related Changes in Tendon Matrix Composition, with Age- and Injury-specific Matrix Fragmentation.

Fascicles from energy-storing tendons show an age-specific response to cyclic fatigue loading (2014)
Journal Article
Thorpe, C. T., Riley, G. P., Birch, H. L., Clegg, P. D., & Screen, H. R. C. (2014). Fascicles from energy-storing tendons show an age-specific response to cyclic fatigue loading. https://doi.org/10.1098/rsif.2013.1058

Some tendons, such as the human Achilles and equine superficial digital flexor tendon (SDFT), act as energy stores, stretching and recoiling to increase efficiency during locomotion. Our previous observations of rotation in response to applied strain... Read More about Fascicles from energy-storing tendons show an age-specific response to cyclic fatigue loading.