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Heterogeneity of proteome dynamics between connective tissue phases of adult tendon

Choi, H; Simpson, D; Wang, D; Prescott, M; Pitsillides, A A; Dudhia, J; Clegg, P D; Ping, P; Thorpe, C T

Authors

H Choi

D Simpson

D Wang

M Prescott

A A Pitsillides

J Dudhia

P D Clegg

P Ping

C T Thorpe



Abstract

Maintenance of connective tissue integrity is fundamental to sustain function, requiring protein turnover to repair damaged tissue. However, connective tissue proteome dynamics remain largely undefined, as do differences in turnover rates of individual proteins in the collagen and glycoprotein phases of connective tissue extracellular matrix (ECM). Here, we investigate proteome dynamics in the collagen and glycoprotein phases of connective tissues by exploiting the spatially distinct fascicular (collagen-rich) and interfascicular (glycoprotein-rich) ECM phases of tendon. Using isotope labelling, mass spectrometry and bioinformatics, we calculate turnover rates of individual proteins within rat Achilles tendon and its ECM phases. Our results demonstrate complex proteome dynamics in tendon, with ~1000 fold differences in protein turnover rates, and overall faster protein turnover within the glycoprotein-rich interfascicular matrix compared to the collagen-rich fascicular matrix. These data provide insights into the complexity of proteome dynamics in tendon, likely required to maintain tissue homeostasis.

Citation

Choi, H., Simpson, D., Wang, D., Prescott, M., Pitsillides, A. A., Dudhia, J., …Thorpe, C. T. (2020). Heterogeneity of proteome dynamics between connective tissue phases of adult tendon. eLife, 9, https://doi.org/10.7554/elife.55262

Journal Article Type Article
Acceptance Date Apr 16, 2020
Publication Date May 12, 2020
Deposit Date May 19, 2020
Publicly Available Date Nov 20, 2020
Journal eLife
Publisher eLife Sciences Publications
Peer Reviewed Peer Reviewed
Volume 9
DOI https://doi.org/10.7554/elife.55262
Public URL https://rvc-repository.worktribe.com/output/1377334

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