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Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation
Journal Article
Orriss, I. R., Key, M. L., Hajjawi, M. O. R., & Arnett, T. R. (in press). Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation. PLoS ONE, 8(7), e69057. https://doi.org/10.1371/journal.pone.0069057

Previous studies have shown that exogenous ATP (>1µM) prevents bone formation in vitro by blocking mineralisation of the collagenous matrix. This effect is thought to be mediated via both P2 receptor-dependent pathways and a receptor-independent mech... Read More about Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation.

Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures
Journal Article
Wornham, D. P., Hajjawi, M. O. R., Orriss, I. R., & Arnett, T. R. (in press). Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures. Osteoporosis International, 25(10), 2477-2484. https://doi.org/10.1007/s00198-014-2791-5

The basic mechanisms by which strontium ranelate acts on bone are still unclear. We show that an important action of strontium salts is to block calcification in cultures of osteoblasts, the bone-forming cells. These results suggest that strontium tr... Read More about Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures.

Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats
Journal Article
Orriss, I. R., Hajjawi, M. O. R., Huesa, C., MacRae, V. E., & Arnett, T. R. (in press). Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats. International Journal of Molecular Medicine, 34(5), 1201-1208. https://doi.org/10.3892/ijmm.2014.1926

The in vitro culture of calvarial osteoblasts from neonatal rodents remains an important method for studying the regulation of bone formation. The widespread use of transgenic mice has created a particular need for a reliable, simple method that allo... Read More about Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats.

Spina bifida-predisposing heterozygous mutations in Planar Cell Polarity genes and Zic2 reduce bone mass in young mice
Journal Article
Orriss, I. R., Lanham, S. A., Savery, D., Greene, N. D. E., Stanier, P., Oreffo, R., …Galea, G. L. (in press). Spina bifida-predisposing heterozygous mutations in Planar Cell Polarity genes and Zic2 reduce bone mass in young mice. https://doi.org/10.1038/s41598-018-21718-x

Fractures are a common comorbidity in children with the neural tube defect (NTD) spina bifida. Mutations in the Wnt/planar cell polarity (PCP) pathway contribute to NTDs in humans and mice, but whether this pathway independently determines bone mass... Read More about Spina bifida-predisposing heterozygous mutations in Planar Cell Polarity genes and Zic2 reduce bone mass in young mice.