Rat osteoblast cultures
Journal Article
Orriss, I. R., Taylor, S. E., & Arnett, T. R. Rat osteoblast cultures. https://doi.org/10.1007/978-1-61779-415-5_3
All Outputs (38)
The regulation of osteoblast function and bone mineralisation by extracellular nucleotides: The role of p2x receptors
Journal Article
Orriss, I. R., Key, M. L., Brandao-Burch, A., Patel, J. J., Burnstock, G., & Arnett, T. R. The regulation of osteoblast function and bone mineralisation by extracellular nucleotides: The role of p2x receptors. BONE, 51(3), 389-400. https://doi.org/10.1016/j.bone.2012.06.013
Rodent osteoclast cultures
Journal Article
Orriss, I. R., & Arnett, T. R. Rodent osteoclast cultures. https://doi.org/10.1007/978-1-61779-415-5_8
The P2X7 receptor is an important regulator of extracellular ATP levels
Journal Article
Brandao-Burch, A., Key, M. L., Patel, J. J., Arnett, T. R., & Orriss, I. R. The P2X7 receptor is an important regulator of extracellular ATP levels. Frontiers in Endocrinology, 3(41), https://doi.org/10.3389/fendo.2012.00041
Role of the P2Y13 receptor in the differentiation of bone marrow stromal cells into osteoblasts and adipocytes
Journal Article
Biver, G., Wang, N., Gartland, A., Orriss, I. R., Arnett, T. R., Boeynaems, J. M., & Robaye, B. Role of the P2Y13 receptor in the differentiation of bone marrow stromal cells into osteoblasts and adipocytes. STEM CELLS, https://doi.org/10.1002/stem.1411
Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation
Journal Article
Orriss, I. R., Key, M. L., Hajjawi, M. O. R., & Arnett, T. R. (in press). Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation. PLoS ONE, 8(7), e69057. https://doi.org/10.1371/journal.pone.0069057Previous studies have shown that exogenous ATP (>1µM) prevents bone formation in vitro by blocking mineralisation of the collagenous matrix. This effect is thought to be mediated via both P2 receptor-dependent pathways and a receptor-independent mech... Read More about Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation.
Generation of rodent and human osteoblasts
Journal Article
Taylor, S. E., Shah, M., & Orriss, I. R. Generation of rodent and human osteoblasts. https://doi.org/10.1038/bonekey.2014.80
FIBRILLIN-1 EXPRESSION, WHICH REGULATES OF TGF-B BIOAVAILABILITY, IS MODIFIED DURING OSTEOARTHRITIS AND MUTATIONS LEAD TO OSTEOARTHRITIS
Journal Article
Ramanayake, W., Jones, H., Orriss, I. R., Arnett, T., Pitsillides, A. A., Denton, C., …Poulet, B. FIBRILLIN-1 EXPRESSION, WHICH REGULATES OF TGF-B BIOAVAILABILITY, IS MODIFIED DURING OSTEOARTHRITIS AND MUTATIONS LEAD TO OSTEOARTHRITIS. Osteoarthritis and Cartilage, 22, S141-S141
THE REGULATION OF SKELETAL AND SOFT TISSUE MINERALIZATION BY ATP AND NPP1
Journal Article
Orriss, I. R., Hajjawi, M. O. R., & Arnett, T. R. THE REGULATION OF SKELETAL AND SOFT TISSUE MINERALIZATION BY ATP AND NPP1. Purinergic Signalling, 10(4), 704-704. https://doi.org/10.1007/s11302-014-9430-7
Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures
Journal Article
Wornham, D. P., Hajjawi, M. O. R., Orriss, I. R., & Arnett, T. R. (in press). Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures. Osteoporosis International, 25(10), 2477-2484. https://doi.org/10.1007/s00198-014-2791-5The basic mechanisms by which strontium ranelate acts on bone are still unclear. We show that an important action of strontium salts is to block calcification in cultures of osteoblasts, the bone-forming cells. These results suggest that strontium tr... Read More about Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures.
Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats
Journal Article
Orriss, I. R., Hajjawi, M. O. R., Huesa, C., MacRae, V. E., & Arnett, T. R. (in press). Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats. International Journal of Molecular Medicine, 34(5), 1201-1208. https://doi.org/10.3892/ijmm.2014.1926The in vitro culture of calvarial osteoblasts from neonatal rodents remains an important method for studying the regulation of bone formation. The widespread use of transgenic mice has created a particular need for a reliable, simple method that allo... Read More about Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats.
Strontium and osteoblast function
Journal Article
Wornham, D., Hajjawi, M. O. R., Orriss, I. R., & Arnett, T. R. Strontium and osteoblast function. Osteoporosis International, https://doi.org/10.1007/s00198-015-3120-3
FIBRILLIN-1 IS EXPRESSED IN CARTILAGE AND MUTATION LEADS TO OA DEVELOPMENT
Journal Article
Ramanayake, W., Jones, H., Orriss, I. R., Arnett, T., Pitsillides, A. A., Denton, C., …Poulet, B. FIBRILLIN-1 IS EXPRESSED IN CARTILAGE AND MUTATION LEADS TO OA DEVELOPMENT. International Journal of Experimental Pathology, 96(2), A4-A5
The role of purinergic signalling in the musculoskeletal system
Journal Article
Orriss, I. R. (in press). The role of purinergic signalling in the musculoskeletal system. https://doi.org/10.1016/j.autneu.2015.04.013
Allopurinol and oxypurinol promote osteoblast differentiation and increase bone formation
Journal Article
Orriss, I. R., Arnett, T. R., George, J., & Witham, M. D. (in press). Allopurinol and oxypurinol promote osteoblast differentiation and increase bone formation. Experimental Cell Research, 342(2), 166-174. https://doi.org/10.1016/j.yexcr.2016.03.004
Design and fabrication of 3D-printed anatomically shaped lumbar cage for intervertebra disc (IVD) degeneration treatment
Journal Article
Serra, T., Capelli, C., Toumpaniari, R., Orriss, I. R., Leong, J., Dalgarno, K., & Kalaskar, D. (in press). Design and fabrication of 3D-printed anatomically shaped lumbar cage for intervertebra disc (IVD) degeneration treatment. Biofabrication, 8(3), https://doi.org/10.1088/1758-5090/8/3/035001
Spina bifida-predisposing heterozygous mutations in Planar Cell Polarity genes and Zic2 reduce bone mass in young mice
Journal Article
Orriss, I. R., Lanham, S. A., Savery, D., Greene, N. D. E., Stanier, P., Oreffo, R., …Galea, G. L. (in press). Spina bifida-predisposing heterozygous mutations in Planar Cell Polarity genes and Zic2 reduce bone mass in young mice. https://doi.org/10.1038/s41598-018-21718-xFractures are a common comorbidity in children with the neural tube defect (NTD) spina bifida. Mutations in the Wnt/planar cell polarity (PCP) pathway contribute to NTDs in humans and mice, but whether this pathway independently determines bone mass... Read More about Spina bifida-predisposing heterozygous mutations in Planar Cell Polarity genes and Zic2 reduce bone mass in young mice.
The in vitro behaviour of canine osteoblasts derived from different bone types
Journal Article
Meeson, R. L., Perpétuo, I. P., Parsons, K. J., Orriss, I. R., Shah, M., Pitsillides, A. A., & Doube, M. (in press). The in vitro behaviour of canine osteoblasts derived from different bone types. BMC Veterinary Research, 15(114), https://doi.org/10.1186/s12917-019-1857-1