Skip to main content

Research Repository

Advanced Search

Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing (2018)
Journal Article
Bossé, J. T., Li, Y., Fernandez Crespo, R., Lacouture, S., Gottschalk, M., Sárközi, R., …Langford, P. R. (2018). Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing. Veterinary Microbiology, 220, 83-89. https://doi.org/10.1016/j.vetmic.2018.05.011

Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were devel... Read More about Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.

Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis (2018)
Journal Article
Bossé, J. T., Li, Y., Sárközi, R., Fodor, L., Lacouture, S., Gottschalk, M., …Langford, P. R. (2018). Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis. Veterinary Microbiology, 217, 1-6. https://doi.org/10.1016/j.vetmic.2018.02.019

The aim of this study was to investigate isolates of Actinobacillus pleuropneumoniae previously designated serologically either as NT or as ‘K2:07’, which did not produce serovar-specific amplicons in PCR assays. We used whole genome sequencing to... Read More about Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis.

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae (2018)
Journal Article
Antenucci, F., Fougeroux, C., Deeney, A., Orskov, C., Rycroft, A. N., Holst, P. J., & Bojesen, A. M. (2018). In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae. Veterinary Research, 49, 4. https://doi.org/10.1186/s13567-017-0502-x

Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a Gram-negative bacterium that represents the main cause of porcine pleuropneumonia in pigs, causing significant economic losses to the livestock industry worldwide. A. pleuropneumoniae, as the... Read More about In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae.

Use of proteins identified through a functional genomic screen to develop a protein subunit vaccine that provides significant protection against virulent Streptococcus suis in pigs (2018)
Journal Article
Brockmeier, S. L., Loving, C. L., Nicholson, T. L., Wang, J., Peters, S. E., Weinert, L., …Tucker, A. W. (2018). Use of proteins identified through a functional genomic screen to develop a protein subunit vaccine that provides significant protection against virulent Streptococcus suis in pigs. Infection and Immunity, 86(3), e00559. https://doi.org/10.1128/IAI.00559-17

Streptococcus suis is a bacterium that is commonly carried in the respiratory tract and that is also one of the most important invasive pathogens of swine, commonly causing meningitis, arthritis, and septicemia. Due to the existence of many serotypes... Read More about Use of proteins identified through a functional genomic screen to develop a protein subunit vaccine that provides significant protection against virulent Streptococcus suis in pigs.

Zoonotic potential of Salmonella enterica carried by pet tortoises (2017)
Journal Article
Bruce, H. L., Barrow, P. A., & Rycroft, A. N. (2017). Zoonotic potential of Salmonella enterica carried by pet tortoises. Veterinary Record, 182(5), 141. https://doi.org/10.1136/vr.104457

The prevalence of Salmonella in chelonians is not known in the UK and it is not clear whether such Salmonella strains would be pathogenic for human beings. Some strains, such as members of the Arizonae subgroup, may be unable to cause anything more t... Read More about Zoonotic potential of Salmonella enterica carried by pet tortoises.

Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014 (2017)
Journal Article
Hernandez-Garcia, J., Wang, J., Restif, O., Holmes, M. A., Mather, A. E., Weinert, L. A., …Tucker, A. W. (2017). Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014. Veterinary Microbiology, 207, 117-124. https://doi.org/10.1016/j.vetmic.2017.06.002

Antimicrobial resistance in Streptococcus suis, a global zoonotic pathogen of pigs, has been mostly studied only in diseased animals using surveys that have not evaluated changes over time. We compared patterns of resistance between S. suis isolat... Read More about Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014.

Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae (2017)
Journal Article
Bossé, J. T., Li, Y., Rogers, J., Fernandez Crespo, R., Li, Y., Chaudhuri, R. R., …Langford, P. R. (2017). Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae. Frontiers in Microbiology, 8(311), https://doi.org/10.3389/fmicb.2017.00311

The aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole... Read More about Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae.

A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay (2017)
Journal Article
Bossé, J. T., Li, Y., Sárközi, R., Gottschalk, M., Angen, Ø., Nedbalcova, K., …Langford, P. R. (2017). A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay. Journal of Clinical Microbiology, 55(3), 902-907. https://doi.org/10.1128/JCM.02166-16

Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a p... Read More about A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay.

Complete Genome Sequence of MIDG2331, a Genetically Tractable Serovar 8 Clinical Isolate of Actinobacillus pleuropneumoniae (2016)
Journal Article
Bossé, J. T., Chaudhuri, R. R., Li, Y., Leanse, L. G., Fernandez Crespo, R., Coupland, P., …Langford, P. R. (2016). Complete Genome Sequence of MIDG2331, a Genetically Tractable Serovar 8 Clinical Isolate of Actinobacillus pleuropneumoniae. Genome Announcements, 4(i), e01667-15. https://doi.org/10.1128/genomeA.01667-15

We report here the complete annotated genome sequence of a clinical serovar 8 isolate Actinobacillus pleuropneumoniae MIDG2331. Unlike the serovar 8 reference strain 405, MIDG2331 is amenable to genetic manipulation via natural transformation as well... Read More about Complete Genome Sequence of MIDG2331, a Genetically Tractable Serovar 8 Clinical Isolate of Actinobacillus pleuropneumoniae.

Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro
Journal Article
Deeney, A. S., Maglennon, G. A., Chapat, L., Crussard, S., Jolivet, E., & Rycroft, A. N. (in press). Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro. Veterinary Research, 50(51), https://doi.org/10.1186/s13567-019-0667-6

Mycoplasma hyopneumoniae, the agent of porcine enzootic pneumonia (EP), is able to persist in the lung tissue and evade destruction by the host for several weeks. To understand the mechanism of pathogen survival, phagocytic uptake of M. hyopneumoniae... Read More about Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro.

Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12
Journal Article
Bossé, J. T., Li, Y., Angen, Ø., Weinert, L. A., Chaudhuri, R. R., Holden, M. T., …Langford, P. R. (in press). Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12. Journal of Clinical Microbiology, 52, 2380-2385. https://doi.org/10.1128/JCM.00685-14

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative am... Read More about Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12.