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A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay

Bossé, J T; Li, Y; Sárközi, R; Gottschalk, M; Angen, Ø; Nedbalcova, K; Rycroft, A N; Fodor, L; Langford, P R

Authors

J T Bossé

Y Li

R Sárközi

M Gottschalk

Ø Angen

K Nedbalcova

A N Rycroft

L Fodor

P R Langford



Abstract

Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic/commensal bacteria of pigs. Analysis of the capsule locus of strain A-85/14 combined with the previous serological data show the existence of a sixteenth serovar—designated serovar 16—of A. pleuropneumoniae.

Citation

Bossé, J. T., Li, Y., Sárközi, R., Gottschalk, M., Angen, Ø., Nedbalcova, K., …Langford, P. R. (2017). A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay. Journal of Clinical Microbiology, 55(3), 902-907. https://doi.org/10.1128/JCM.02166-16

Journal Article Type Article
Acceptance Date Dec 22, 2016
Publication Date Jan 4, 2017
Deposit Date Jun 1, 2017
Publicly Available Date Jun 1, 2017
Journal JOURNAL OF CLINICAL MICROBIOLOGY
Print ISSN 0095-1137
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 55
Issue 3
Pages 902-907
DOI https://doi.org/10.1128/JCM.02166-16
Public URL https://rvc-repository.worktribe.com/output/1393994

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