A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay

Bossé, J T; Li, Y; Sárközi, R; Gottschalk, M; Angen, Ø; Nedbalcova, K; Rycroft, A N; Fodor, L; Langford, P R

Abstract

Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic/commensal bacteria of pigs. Analysis of the capsule locus of strain A-85/14 combined with the previous serological data show the existence of a sixteenth serovar—designated serovar 16—of A. pleuropneumoniae.

Citation

Bossé, J. T., Li, Y., Sárközi, R., Gottschalk, M., Angen, Ø., Nedbalcova, K., Rycroft, A. N., Fodor, L., & Langford, P. R. (2017). A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay. Journal of Clinical Microbiology, 55(3), 902-907. https://doi.org/10.1128/JCM.02166-16

• Journal Article Type: Article
• Acceptance Date: Dec 22, 2016
• Publication Date: Jan 4, 2017
• Deposit Date: Jun 1, 2017
• Publicly Available Date: Jun 1, 2017
• Journal: JOURNAL OF CLINICAL MICROBIOLOGY
• Print ISSN: 0095-1137
• Electronic ISSN: 1098-660X
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 55
• Issue: 3
• Pages: 902-907
• DOI: https://doi.org/10.1128/JCM.02166-16
• Public URL: https://rvc-repository.worktribe.com/output/1393994