Skip to main content

Research Repository

Advanced Search

Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12

Bossé, J T; Li, Y; Angen, Ø; Weinert, L A; Chaudhuri, R R; Holden, M T; Williamson, S M; Maskell, D J; Tucker, A W; Wren, B W; Rycroft, A N; Langford, P R

Authors

J T Bossé

Y Li

Ø Angen

L A Weinert

R R Chaudhuri

M T Holden

S M Williamson

D J Maskell

A W Tucker

B W Wren

A N Rycroft

P R Langford



Abstract

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

Citation

Bossé, J. T., Li, Y., Angen, Ø., Weinert, L. A., Chaudhuri, R. R., Holden, M. T., …Langford, P. R. (in press). Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12. Journal of Clinical Microbiology, 52, 2380-2385. https://doi.org/10.1128/JCM.00685-14

Journal Article Type Article
Acceptance Date Apr 15, 2014
Deposit Date Nov 12, 2014
Publicly Available Date Nov 30, 2018
Journal JOURNAL OF CLINICAL MICROBIOLOGY
Print ISSN 0095-1137
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 52
Pages 2380-2385
DOI https://doi.org/10.1128/JCM.00685-14
Public URL https://rvc-repository.worktribe.com/output/1405624
Additional Information Corporate Creators : AHVLA, Cambridge, Imperial College London, LSHTM, Norwegian Vet Instit, Wellcome Trust Sanger Institute

Files







You might also like



Downloadable Citations