Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12

Bossé, J T; Li, Y; Angen, Ø; Weinert, L A; Chaudhuri, R R; Holden, M T; Williamson, S M; Maskell, D J; Tucker, A W; Wren, B W; Rycroft, A N; Langford, P R

Abstract

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

Citation

Bossé, J. T., Li, Y., Angen, Ø., Weinert, L. A., Chaudhuri, R. R., Holden, M. T., Williamson, S. M., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., & Langford, P. R. (in press). Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12. Journal of Clinical Microbiology, 52, 2380-2385. https://doi.org/10.1128/JCM.00685-14

• Journal Article Type: Article
• Acceptance Date: Apr 15, 2014
• Deposit Date: Nov 12, 2014
• Publicly Available Date: Nov 30, 2018
• Journal: Journal of Clinical Microbiology
• Print ISSN: 0095-1137
• Electronic ISSN: 1098-660X
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 52
• Pages: 2380-2385
• DOI: https://doi.org/10.1128/JCM.00685-14
• Public URL: https://rvc-repository.worktribe.com/output/1405624
• Additional Information: Corporate Creators : AHVLA, Cambridge, Imperial College London, LSHTM, Norwegian Vet Instit, Wellcome Trust Sanger Institute