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The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture (2020)
Journal Article
Marugan-Hernandez, V., Jeremiah, G., Aguiar-Martins, K., Burrell, A., Vaughan, S., Xia, D., …Tomley, F. (2020). The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture. Frontiers in Cellular and Infection Microbiology, 10, 579833

In vitro development of the complete life cycle of Eimeria species has been achieved in primary cultures of avian epithelial cells with low efficiency. The use of immortalized cell lines simplifies procedures but only allows partial development throu... Read More about The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture.

In vitro Anticoccidial Study of Oregano and Garlic Essential Oils and Effects on Growth Performance, Fecal Oocyst Output, and Intestinal Microbiota in vivo (2020)
Journal Article
Sidiropoulou, E., Skoufos, I., Marugan-Hernandez, V., Giannenas, I., Bonos, E., Aguiar-Martins, K., …Tzora, A. (2020). In vitro Anticoccidial Study of Oregano and Garlic Essential Oils and Effects on Growth Performance, Fecal Oocyst Output, and Intestinal Microbiota in vivo. Frontiers in Veterinary Science, 7, https://doi.org/10.3389/fvets.2020.00420

This study investigated the in vitro effects of Greek oregano and garlic essential oils on inhibition of Eimeria parasites and their in vivo effects on production performance, intestinal bacteria counts, and oocyst output. An inhibition assay was per... Read More about In vitro Anticoccidial Study of Oregano and Garlic Essential Oils and Effects on Growth Performance, Fecal Oocyst Output, and Intestinal Microbiota in vivo.

Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high level E. maxima challenge in a broiler model of coccidiosis. (2020)
Journal Article
Pastor-Fernández, I., Sungwon, K., Marugán-Hernández, V., Soutter, F., Tomley, F., & Blake, D. (2020). Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high level E. maxima challenge in a broiler model of coccidiosis. Parasites and Vectors,

Poultry coccidiosis is a parasitic enteric disease with a highly negative impact on chicken production. In-feed chemoprophylaxis remains the primary method of control, but the increasing ineffectiveness of anticoccidial drugs, and potential future re... Read More about Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high level E. maxima challenge in a broiler model of coccidiosis..

Life cycle stages, specific organelles and invasion mechanisms of Eimeria species (2019)
Journal Article
Burrell, A., Tomley, F. M., Vaughan, S., & Marugan-Hernandez, V. (2019). Life cycle stages, specific organelles and invasion mechanisms of Eimeria species. Parasitology, https://doi.org/10.1017/S0031182019001562

Apicomplexans, including species of Eimeria, pose a real threat to the health and wellbeing of animals and humans. Eimeria parasites do not infect humans but cause an important economic impact on livestock, in particular on the poultry industry. Desp... Read More about Life cycle stages, specific organelles and invasion mechanisms of Eimeria species.

Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens (2018)
Journal Article
Pastor-Fernández, I., Kim, S., Billington, K., Bumstead, J., Marugán-Hernández, V., Küster, T., …Tomley, F. M. (2018). Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens. International Journal for Parasitology, 48(7), 505-518. https://doi.org/10.1016/j.ijpara.2018.01.003

Recently, the availability of protocols supporting genetic complementation of Eimeria has raised the prospect of generating transgenic parasite lines which can function as vaccine vectors, expressing and delivering heterologous proteins. Complementat... Read More about Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens.

Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella (2017)
Journal Article
Marugan-Hernandez, V., Fiddy, R., Nurse-Francis, J., Smith, O., Pritchard, L., & Tomley, F. M. (2017). Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella. https://doi.org/10.1186/s13071-017-2454-4

In this study, we evaluated the binding capacity of Type I MAR domains from novel E. tenella MCPs. Variants of the previously described HxT motif were analysed showing that HxT and VxT variants bind, whereas HxS and YxE variants did not. One of these... Read More about Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella.

Neospora caninum and Bovine Neosporosis: Current Vaccine Research (2017)
Journal Article
Marugan-Hernandez, V. (2017). Neospora caninum and Bovine Neosporosis: Current Vaccine Research. Journal of Comparative Pathology, 157(2-3), 193-200. https://doi.org/10.1016/j.jcpa.2017.08.001

Neospora caninum, a tissue cyst-forming parasite, is the causative agent of bovine neosporosis. It is considered to be one of the most important transmissible causes of reproductive failure in cattle; abortion and neonatal mortality result in signifi... Read More about Neospora caninum and Bovine Neosporosis: Current Vaccine Research.

Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle (2017)
Journal Article
Marugan-Hernandez, V., Long, E., Blake, D. P., Crouch, C., & Tomley, F. M. (2017). Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle. https://doi.org/10.1038/s41598-017-04049-1

Eimeria spp. are intracellular parasites that have a major impact on poultry. Effective live vaccines are available and the development of reverse genetic technologies has raised the prospect of using Eimeria spp. as recombinant vectors to express ad... Read More about Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle.