Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection by

Regidor-Cerrillo, J; Xia, D; Jiménez-Pelayo, L; García-Sánchez, M; Collantes-Fernández, E; Randle, N; Wastling, J; Ortega-Mora, L M; Horcajo, P

Abstract

Despite the importance of bovine neosporosis, relevant knowledge gaps remain concerning the pathogenic mechanisms of . Infection of the placenta is a crucial event in the pathogenesis of the disease; however, very little is known about the relation of the parasite with this target organ. Recent studies have shown that isolates with important variations in virulence also show different interactions with the bovine trophoblast cell line F3 in terms of proliferative capacity and transcriptome host cell modulation. Herein, we used the same model of infection to study the interaction of with these target cells at the proteomic level using LC-MS/MS over the course of the parasite lytic cycle. We also analysed the proteome differences between high- (Nc-Spain7) and low-virulence (Nc-Spain1H) isolates. The results showed that mitochondrial processes and metabolism were the main points of -host interactions. Interestingly, Nc-Spain1H infection showed a higher level of influence on the host cell proteome than Nc-Spain7 infection.

Citation

Regidor-Cerrillo, J., Xia, D., Jiménez-Pelayo, L., García-Sánchez, M., Collantes-Fernández, E., Randle, N., Wastling, J., Ortega-Mora, L. M., & Horcajo, P. (2020). Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection by. Pathogens, 9(9),

• Journal Article Type: Article
• Acceptance Date: Sep 11, 2020
• Publication Date: Sep 15, 2020
• Deposit Date: Oct 29, 2020
• Publicly Available Date: Oct 29, 2020
• Journal: Pathogens (Basel, Switzerland)
• Print ISSN: 2076-0817
• Electronic ISSN: 2076-0817
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 9
• Keywords: Neospora caninum, bovine trophoblast cell line, host–parasite interaction, isolate virulence, proteome
• Public URL: https://rvc-repository.worktribe.com/output/1375708