Characterisation of Canine Intestinal MicroRNA Expression in Inflammatory Bowel Disease and T-Cell Lymphoma

Williams, Jonathan; Werling, Dirk; Davison, Lucy; Hiron, Thomas; Xia, Dong

Abstract

Differentiating between canine inflammatory bowel disease and intestinal T-cell lymphoma via histopathological examination of endoscopically-derived intestinal biopsies can be challenging and involves an invasive procedure requiring specialised equipment and training. A rapid, non-invasive method of diagnosis, such as blood or faecal analysis for a conserved and stable biomarker would be a useful adjunct or replacement. Studies on dogs and humans with various types of lymphoma have shown altered microRNA patterns in blood, faeces and tissues indicating their potential use as biomarkers of disease. The present study aimed to differentiate between normal/minimally inflamed canine duodenal tissue taken from dogs with either severe inflammatory bowel disease and T-cell lymphoma using next generation sequencing and qPCR validation to elucidate differentially expressed microRNAs in formalin-fixed paraffin-embedded (FFPE) endoscopically-derived canine duodenum. Our results show that miRNA can be extracted from archived endoscopically derived FFPE tissues from the canine duodenum and used to differentiate normal/minimally inflamed canine duodenal tissue from severe lymphoplasmacytic inflammatory bowel disease and from T-cell lymphoma.

Citation

Williams, J., Werling, D., Davison, L., Hiron, T., & Xia, D. (in press). Characterisation of Canine Intestinal MicroRNA Expression in Inflammatory Bowel Disease and T-Cell Lymphoma. Journal of Comparative Pathology, https://doi.org/10.1016/j.jcpa.2023.03.186

• Journal Article Type: Article
• Acceptance Date: Mar 30, 2023
• Online Publication Date: Jun 20, 2023
• Deposit Date: Apr 21, 2023
• Publicly Available Date: Jun 20, 2023
• Journal: Journal of Comparative Pathology
• Print ISSN: 0021-9975
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1016/j.jcpa.2023.03.186