Phenotypic heterogeneity of peripheral monocytes in healthy dogs

Gibbons, N; Goulart, M R; Chang, Y M; Efstathiou, K; Purcell, R; Wu, Y; Peters, L M; Turmaine, M; Szladovits, B; Garden, O A

doi:10.1016/j.vetimm.2017.06.007

Authors

N Gibbons

M R Goulart

Y M Chang

K Efstathiou

R Purcell

Y Wu

L M Peters

M Turmaine

B Szladovits

O A Garden

Abstract

Monocytes are key cells of the innate immune system. Their phenotypic and functional roles have been investigated in humans, mice and other animals, such as the rat, pig and cow. To date, detailed phenotypic analysis of monocytes has not been undertaken in dogs. Two important surface markers in human monocytes are CD14 and MHC class II (MHC II). By flow cytometry, we demonstrated that canine monocytes can be subdivided into three separate populations: CD14posMHC IIneg, CD14posMHC IIpos and CD14negMHC IIpos. Both light and transmission electron microscopy confirmed the monocytic identity of all three populations. The CD14posMHC IIneg population could be distinguished on an ultrastructural level by their smaller size, the presence of more numerous, larger granules, and more pseudopodia than both of the other populations.

Citation

Gibbons, N., Goulart, M. R., Chang, Y. M., Efstathiou, K., Purcell, R., Wu, Y., …Garden, O. A. (2017). Phenotypic heterogeneity of peripheral monocytes in healthy dogs. Veterinary Immunology and Immunopathology, 190, 26-30. https://doi.org/10.1016/j.vetimm.2017.06.007

Journal Article Type	Other
Acceptance Date	Jun 19, 2017
Publication Date	Jun 28, 2017
Deposit Date	Jul 4, 2017
Publicly Available Date	Jun 29, 2018
Journal	VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Print ISSN	0165-2427
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	190
Pages	26-30
DOI	https://doi.org/10.1016/j.vetimm.2017.06.007
Public URL	https://rvc-repository.worktribe.com/output/1391388