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Phenotypic heterogeneity of peripheral monocytes in healthy dogs

Gibbons, N; Goulart, M R; Chang, Y M; Efstathiou, K; Purcell, R; Wu, Y; Peters, L M; Turmaine, M; Szladovits, B; Garden, O A


N Gibbons

M R Goulart

Y M Chang

K Efstathiou

R Purcell

Y Wu

L M Peters

M Turmaine

B Szladovits

O A Garden


Monocytes are key cells of the innate immune system. Their phenotypic and functional roles have been investigated in humans, mice and other animals, such as the rat, pig and cow. To date, detailed phenotypic analysis of monocytes has not been undertaken in dogs. Two important surface markers in human monocytes are CD14 and MHC class II (MHC II). By flow cytometry, we demonstrated that canine monocytes can be subdivided into three separate populations: CD14posMHC IIneg, CD14posMHC IIpos and CD14negMHC IIpos. Both light and transmission electron microscopy confirmed the monocytic identity of all three populations. The CD14posMHC IIneg population could be distinguished on an ultrastructural level by their smaller size, the presence of more numerous, larger granules, and more pseudopodia than both of the other populations.


Gibbons, N., Goulart, M. R., Chang, Y. M., Efstathiou, K., Purcell, R., Wu, Y., …Garden, O. A. (2017). Phenotypic heterogeneity of peripheral monocytes in healthy dogs. Veterinary Immunology and Immunopathology, 190, 26-30.

Journal Article Type Other
Acceptance Date Jun 19, 2017
Publication Date Jun 28, 2017
Deposit Date Jul 4, 2017
Publicly Available Date Jul 1, 2018
Print ISSN 0165-2427
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 190
Pages 26-30
Public URL