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Cultured dissociated primary dorsal root ganglion neurons from adult horses enable study of axonal transport

Adalbert, Robert; Cahalan, Stephen; Hopkins, Eleanor; Almuhanna, Abdulaziz; Loreto, Andrea; Por, Erzsebet; Perkins, Justin; Coleman, Michael; Piercy, Richard

Authors

Robert Adalbert

Stephen Cahalan

Eleanor Hopkins

Abdulaziz Almuhanna

Andrea Loreto

Erzsebet Por

Justin Perkins

Michael Coleman

Richard Piercy



Abstract

Neurological disorders are prevalent in horses, but their study is challenging due to anatomic constraints and the large body size; very few host-specific in vitro models have been established to study these types of diseases, particularly from adult donor tissue. Here we report generation of primary neuronal dorsal root ganglia (DRG) cultures from adult horses: the mixed, dissociated cultures, containing neurons and glial cells, remained viable for 90 days. Similar to DRG neurons in vivo, cultured neurons varied in size and they developed long neurites. Mitochondrial movement was detected in cultured cells and was found to be significantly slower in glial cells compared to DRG-derived neurons. In addition, mitochondria were more elongated in glial cells than those in neurons. Our culture model will be a useful tool to study the contribution of axonal transport defects to specific neurodegenerative diseases in horses.

Citation

Adalbert, R., Cahalan, S., Hopkins, E., Almuhanna, A., Loreto, A., Por, E., …Piercy, R. (2022). Cultured dissociated primary dorsal root ganglion neurons from adult horses enable study of axonal transport. Journal of Anatomy, https://doi.org/10.1111/joa.13719

Journal Article Type Article
Acceptance Date Jun 7, 2022
Publication Date Jun 21, 2022
Deposit Date Nov 2, 2021
Publicly Available Date Jul 15, 2022
Journal Journal of Anatomy
Print ISSN 0021-8782
Publisher Wiley
Peer Reviewed Not Peer Reviewed
DOI https://doi.org/10.1111/joa.13719

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