Skip to main content

Research Repository

Advanced Search

Prospective evaluation of novel biomarkers of acute kidney injury in dogs following cardiac surgery under cardiopulmonary bypass

Starybrat, Daria; Jepson, Rosanne; Bristow, Poppy; Peterson, Sarah; Yerramilli, Maha; Yerramilli, Murthy; Chang, Yu-Mei; Cortellini, Stefano


Daria Starybrat

Rosanne Jepson

Poppy Bristow

Sarah Peterson

Maha Yerramilli

Murthy Yerramilli

Yu-Mei Chang

Stefano Cortellini


Objective - Assess the occurrence of acute kidney injury (AKI) in dogs undergoing cardiac surgery under cardiopulmonary bypass (CPB) and explore associations between traditional and novel serum and urinary biomarkers.
Design – Prospective cohort study conducted between July 2018 and April 2019.
Setting – University teaching hospital. Animals - Nineteen dogs undergoing cardiac surgery under CPB with preoperative serum creatinine < 140 μmol/l (< 1.6 mg/dl). Interventions - Blood and urine samples were obtained at 4 points: preoperatively following general anesthesia induction, immediately postoperatively, 2 and 4 days postoperatively (T1, T2, T3 and T4). AKI was defined as an increase in serum creatinine ≥ 26.4 μmol/l (≥ 0.3 mg/dl) above baseline within 48 hours. Serum creatinine, C-Reactive Protein (CRP), symmetric dimethylarginine (SDMA), inosine, beta-aminoisobutyric acid (BAIB), urinary clusterin (uClus) and urinary cystatin B (uCysB) were measured. Data were log-transformed (log10) when appropriate and assessed using linear mixed effects models. Measurements and Main Results - AKI occurred in 3/19 dogs (15.8%, 95% CI, 0.047 to 0.384). Inosine increased at T2 (adjusted mean ± standard error, 53±5.6) in all dogs, and then gradually decreased. Log10uCysB increased at T2 (2.3±0.1) in all dogs and remained high. Log10CRP and log10uClus increased significantly at T3 (1.9±0.1 and 3.6±0.1, respectively) in all dogs and remained elevated. There was a significant positive association between serum creatinine and SDMA (P < 0.001, estimate ± standard error 0.06±.00), between log10CRP and log10uClus (P < 0.001, 0.35±0.08), between SDMA and creatinine as well as between SDMA and BAIB (P < 0.001, 11.1±0.83 and P < 0.001, 1.06±0.22, respectively) for all dogs at all time points. Conclusions - Inosine and uCysB concentrations changed in all dogs immediately following a surgery under CPB and may indicate tubular injury. Further studies are required to ascertain the usefulness of those biomarkers in early detection of AKI.


Starybrat, D., Jepson, R., Bristow, P., Peterson, S., Yerramilli, M., Yerramilli, M., …Cortellini, S. (in press). Prospective evaluation of novel biomarkers of acute kidney injury in dogs following cardiac surgery under cardiopulmonary bypass. Journal of Veterinary Emergency and Critical Care,

Journal Article Type Article
Acceptance Date Nov 12, 2021
Online Publication Date Feb 9, 2022
Deposit Date Nov 25, 2021
Publicly Available Date Nov 12, 2021
Journal Journal Veterinary Emergency and Critical Care
Print ISSN 1479-3261
Publisher Wiley
Peer Reviewed Peer Reviewed
Public URL


You might also like

Downloadable Citations