Topical application of cervix with hyaluronan improves fertility in goats inseminated with frozen-thawed semen

Leethongdee, S; Thuangsanthia, A; Khalid, M

Abstract

Objective: Artificial insemination plays an important role in genetic improvement in the goat farming system. The aim of this study was to investigate the effect of cervical application of hyaluronan (HA) on the fertility in goats after cervical artificial insemination using frozenthawed (F-T) semen. Methods: After oestrous synchronisation with progesterone sponges and pregnant mare serum gonadotropin injection, both nulli- and multi-parous goats, were randomly allocated to 2 groups, and were inseminated with 0.25 mL of F-T semen (150?106 spermatozoa) twice at 52 h and 56 h after sponge removal. Prior to the insemination, goats in Group 1 only were given topical cervical HA application at 48 h after sponge removal. Site of insemination was recorded as os-cervix or intra-cervix or intra-uterus. Pregnancy was tested ultrasonographically 42 days after insemination. The data on pregnancy rates and percentage of animals according to the site of semen deposition were compared by Chi-square analysis. Results: The overall pregnancy rate was significantly (p

Citation

Leethongdee, S., Thuangsanthia, A., & Khalid, M. (2021). Topical application of cervix with hyaluronan improves fertility in goats inseminated with frozen-thawed semen. Animal Bioscience, 34(6), 985-992. https://doi.org/10.5713/ajas.20.0284

• Journal Article Type: Article
• Acceptance Date: Jul 23, 2020
• Publication Date: 2021
• Deposit Date: Dec 15, 2021
• Publisher: Asian-Australasian Association of Animal Production Societies(AAAP)
• Peer Reviewed: Peer Reviewed
• Volume: 34
• Issue: 6
• Pages: 985-992
• DOI: https://doi.org/10.5713/ajas.20.0284
• Keywords: Goat Cervix; Hyaluronan; Cervical Insemination; Frozen-thawed Semen; LAPAROSCOPIC ARTIFICIAL-INSEMINATION; FOLLICLE-STIMULATING-HORMONE; INTRAUTERINE INSEMINATION; RAM SEMEN; REPRODUCTIVE-PERFORMANCE; OVINE CERVIX; OXYTOCIN; EXPRESSION; SHEEP; EWE
• Public URL: https://rvc-repository.worktribe.com/output/1553217