Genetic diversity within the 18S rRNA and actin locus of Cryptosporidium scrofarum (Apicomplexa: Cryptosporidiidae) infecting domestic pigs (Sus scrofa domesticus) of India

Sharma, D; Singh, NK; Singh, H; Rath, SS; Blake, DP

Abstract

The genetic diversity was studied of Cryptosporidium scrofarum (syn Cryptosporidium pig genotype II) of domestic pigs (Sus scrofa domesticus) from Punjab, India. Nested PCR amplification targeting the 18S rRNA and actin gene loci from Cryptosporidium positive samples was carried out, and the amplicons were sequenced. Phylogenetic comparison of a partial 18S rRNA gene revealed that they were genetically most similar to C. scrofarum isolated from other parts of the world. However, comparison of sequences representing a fragment of the genomic actin locus identified a new genotype conserved within the isolates sampled from India but distinct from other published sequences, suggesting the presence of a different Indian genotype.

Citation

Sharma, D., Singh, N., Singh, H., Rath, S., & Blake, D. (2021). Genetic diversity within the 18S rRNA and actin locus of Cryptosporidium scrofarum (Apicomplexa: Cryptosporidiidae) infecting domestic pigs (Sus scrofa domesticus) of India. Veterinarski Arhiv, 91(3), 269-276. https://doi.org/10.24099/vet.arhiv.0674

• Journal Article Type: Article
• Acceptance Date: Mar 12, 2021
• Publication Date: 2021
• Deposit Date: Dec 23, 2021
• Publicly Available Date: Dec 23, 2021
• Publisher: University of Zagreb
• Peer Reviewed: Peer Reviewed
• Volume: 91
• Issue: 3
• Pages: 269-276
• DOI: https://doi.org/10.24099/vet.arhiv.0674
• Keywords: actin; Cryptosporidium scrofarum; genetic diversity; pig; phylogeny; 18S rRNA; MOLECULAR CHARACTERIZATION; GENOTYPE II; ADULT-PIGS; IDENTIFICATION; SUIS; PREVALENCE; PRE
• Public URL: https://rvc-repository.worktribe.com/output/1553421