One Health Approach: Invasive California Kingsnake (Lampropeltis californiae) as an Important Source of Antimicrobial Drug-Resistant Salmonella Clones on Gran Canaria Island

Santana-Hernandez, KM; Rodriguez-Ponce, E; Medina, IR; Acosta-Hernandez, B; Priestnall, SL; Vega, S; Marin, C; Cerda-Cuellar, M; Marco-Fuertes, A; Ayats, T; Beltran, TG; Lupiola-Gomez, PA

Abstract

The aim of this study was to investigate the invasive species Lampropeltis californiae (California kingsnake) as a reservoir of Salmonella and its ability to spread different clones of the bacterium with zoonotic potential into the environment, as well as study its antimicrobial resistance patterns in Gran Canaria (Spain). The main results showed that a high diversity of Salmonella subsp. salamae strains circulate in Gran Canaria with a high prevalence of resistance shown for antimicrobials of public health importance, as summarised in the European Decision 2013/652/EU. Abstract: The increase in the reptile population has led to a rise in the number of zoonotic infections due to close contact with reptiles, with reptile-associated salmonellosis being particularly relevant. California kingsnake invasion not only threatens the endemic reptile population of the island of Gran Canaria (Spain) but also poses serious public health problems by spreading zoonotic pathogens and their antimicrobial resistance (AMR) to the environment. Thus, the aim of this study was to assess the occurrence, genetic diversity, and AMR among Salmonella spp. strains isolated from California kingsnakes in Gran Canaria Island (Spain). Of 73 invasive individuals captured, 20.5% carried Salmonella spp., belonging to different subspecies and serovars, with subsp. salamae as the most abundant. Pulsed-field electrophoresis showed high genetic diversity among subsp. salamae isolates, and among these, 73.3% showed resistance to at least one of the antimicrobials tested. In conclusion, the present study revealed the importance of wild invasive California kingsnakes as reservoirs of drug-resistant Salmonella spp. that could pose a direct threat to livestock and humans. Identification of drug-resistant Salmonella strains in wildlife provides valuable information on potential routes of transmission that involve risks to public and animal health.

Citation

Santana-Hernandez, K., Rodriguez-Ponce, E., Medina, I., Acosta-Hernandez, B., Priestnall, S., Vega, S., Marin, C., Cerda-Cuellar, M., Marco-Fuertes, A., Ayats, T., Beltran, T., & Lupiola-Gomez, P. (2023). One Health Approach: Invasive California Kingsnake (Lampropeltis californiae) as an Important Source of Antimicrobial Drug-Resistant Salmonella Clones on Gran Canaria Island. Animals, 13(11), https://doi.org/10.3390/ani13111790

• Journal Article Type: Article
• Acceptance Date: May 25, 2023
• Online Publication Date: May 28, 2023
• Publication Date: 2023
• Deposit Date: Feb 13, 2024
• Publicly Available Date: Feb 13, 2024
• Print ISSN: 2076-2615
• Electronic ISSN: 2076-2615
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 11
• DOI: https://doi.org/10.3390/ani13111790
• Keywords: Salmonella; multi-drug resistance; PFGE; Lampropeltis californiae; ESCHERICHIA-COLI; PET REPTILES; PREVALENCE; SPP.; INFECTION; SEROVARS; SNAKES; MICE