Sensitivity and specificity of human point-of-care circulating cathodic antigen (POC-CCA) test in African livestock for rapid diagnosis of schistosomiasis: A Bayesian latent class analysis

Calvo-Urbano, B; Leger, E; Gabain, I; De Dood, CJ; Diouf, ND; Borlase, A; Rudge, JW; Corstjens, PLAM; Sene, M; Van Dam, GJ; Walker, M; Webster, JP

Abstract

Schistosomiasis is a major neglected tropical disease (NTD) affecting both humans and animals. The morbidity and mortality inflicted upon livestock in the Afrotropical region has been largely overlooked, in part due to a lack of validated sensitive and specific tests, which do not require specialist training or equipment to deliver and interpret. As stressed within the recent WHO NTD 2021-2030 Roadmap and Revised Guideline for schistosomiasis, inexpensive, non-invasive, and sensitive diagnostic tests for livestock-use would also facilitate both prevalence mapping and appropriate intervention programmes. The aim of this study was to assess the sensitivity and specificity of the currently available point-of-care circulating cathodic antigen test (POC-CCA), designed for Schistosoma mansoni detection in humans, for the detection of intestinal livestock schistosomiasis caused by Schistosoma bovis and Schistosoma curassoni. POC-CCA, together with the circulating anodic antigen (CAA) test, miracidial hatching technique (MHT) and organ and mesentery inspection (for animals from abattoirs only), were applied to samples collected from 195 animals (56 cattle and 139 small ruminants (goats and sheep) from abattoirs and living populations) from Senegal. POC-CCA sensitivity was greater in the S. curassoni-dominated Barkedji livestock, both for cattle (median 81%; 95% credible interval (CrI): 55%-98%) and small ruminants (49%; CrI: 29%-87%), than in S. bovis-dominated Richard Toll ruminants (cattle: 62%; CrI: 41%-84%; small ruminants: 12%, CrI: 1%-37%). Overall, sensitivity was greater in cattle than in small ruminants. Small ruminants POC-CCA specificity was similar in both locations (91%; CrI: 77%-99%), whilst cattle POC-CCA specificity could not be assessed owing to the low number of uninfected cattle surveyed. Our results indicate that, whilst the current POC-CCA does represent a potential diagnostic tool for cattle and possibly for predominantly S. curassoni-infected livestock, future work is needed to develop parasite- and/or livestock-specific affordable and field-applicable diagnostic tests to enable determination of the true extent of livestock schistosomiasis. Author summarySchistosomiasis is a neglected tropical and zoonotic disease, infecting over 230 million people and millions of animals worldwide. The potential contribution of livestock schistosomiasis to disease transmission in human populations has implications for the design of effective disease management and elimination programmes. However, our understanding of the true prevalence and impact of animal schistosomiasis is severely limited, in part due to a lack of accessible and accurate diagnostic tools. This need for sensitive and specific tools for animal schistosomiasis diagnosis has been recognised in the most recent WHO Guideline and NTD roadmap. As a point-of-care circulating cathodic antigen (POC-CCA) diagnostic test is now available to assess intestinal schistosomiasis caused by Schistosoma mansoni in humans, we hypothesised that POC-CCA could be used to detect livestock intestinal schistosomiasis caused by Schistosoma bovis and Schistosoma curassoni. The aim of this study was to evaluate the sensitivity and specificity of POC-CCA for the detection of intestinal livestock schistosomiasis in Senegal. POC-CCA sensitivity varied by ruminant group and by parasite species/location, while POC-CCA specificity in small ruminants, at least, did not vary across populations. We conclude that, whilst the currently-available POC-CCA does represent a potential diagnostic tool for schistosomiasis in cattle, the factors determining test performance warrant further investigation and further livestock-specific assays would be ideal.

Citation

Calvo-Urbano, B., Leger, E., Gabain, I., De Dood, C., Diouf, N., Borlase, A., Rudge, J., Corstjens, P., Sene, M., Van Dam, G., Walker, M., & Webster, J. (2023). Sensitivity and specificity of human point-of-care circulating cathodic antigen (POC-CCA) test in African livestock for rapid diagnosis of schistosomiasis: A Bayesian latent class analysis. PLoS Neglected Tropical Diseases, 17(5), https://doi.org/10.1371/journal.pntd.0010739

• Journal Article Type: Article
• Acceptance Date: Apr 29, 2023
• Online Publication Date: May 22, 2023
• Publication Date: 2023
• Deposit Date: Feb 15, 2024
• Publicly Available Date: Feb 15, 2024
• Print ISSN: 1935-2727
• Electronic ISSN: 1935-2735
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 17
• Issue: 5
• DOI: https://doi.org/10.1371/journal.pntd.0010739
• Keywords: LATERAL-FLOW ASSAY; LOW-ENDEMIC AREA; ANODIC ANTIGEN; CONDITIONAL DEPENDENCE; ANIMAL SCHISTOSOMIASIS; CASSETTE TEST; ERROR RATES; URINE ASSAY; EPIDEMIOLOGY; PREVALENCE