V Lindsay-McGee
Characterisation of phenotypic patterns in equine exercise-associated myopathies
Lindsay-McGee, V; Massey, C; Li, YT; Clark, EL; Psifidi, A; Piercy, RJ
Authors
C Massey
YT Li
EL Clark
A Psifidi
RJ Piercy
Abstract
Background Equine exercise-associated myopathies are prevalent, clinically heterogeneous, generally idiopathic disorders characterised by episodes of myofibre damage that occur in association with exercise. Episodes are intermittent and vary within and between affected horses and across breeds. The aetiopathogenesis is often unclear; there might be multiple causes. Poor phenotypic characterisation hinders genetic and other disease analyses. Objectives The aim of this study was to characterise phenotypic patterns across exercise-associated myopathies in horses. Study designHistorical cross-sectional study, with subsequent masked case-control validation study. Methods Historical clinical and histological features from muscle samples (n = 109) were used for k-means clustering and validated using principal components analysis and hierarchical clustering. For further validation, a blinded histological study (69 horses) was conducted comparing two phenotypic groups with selected controls and horses with histopathological features characterised by myofibrillar disruption. Results We identified two distinct broad phenotypes: a non-classic exercise-associated myopathy syndrome (EAMS) subtype was associated with practitioner-described signs of apparent muscle pain (p < 0.001), reluctance to move (10.85, p = 0.001), abnormal gait (p < 0.001), ataxia (p = 0.001) and paresis (p = 0.001); while a non-specific classic RER subtype was not uniquely associated with any particular variables. No histological differences were identified between subtypes in the validation study, and no identifying histopathological features for other equine myopathies identified in either subtype. Main limitationsLack of an independent validation population; small sample size of smaller identified subtypes; lack of positive control myofibrillar myopathy cases; case descriptions derived from multiple independent and unblinded practitioners. Conclusions This is the first study using computational clustering methods to identify phenotypic patterns in equine exercise-associated myopathies, and suggests that differences in patterns of presenting clinical signs support multiple disease subtypes, with EAMS a novel subtype not previously described. Routine muscle histopathology was not helpful in sub-categorising the phenotypes in our population.
Citation
Lindsay-McGee, V., Massey, C., Li, Y., Clark, E., Psifidi, A., & Piercy, R. (2024). Characterisation of phenotypic patterns in equine exercise-associated myopathies. Equine Veterinary Journal, https://doi.org/10.1111/evj.14128
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 5, 2024 |
Online Publication Date | Jul 5, 2024 |
Publication Date | 2024 |
Deposit Date | Jul 18, 2024 |
Publicly Available Date | Jul 18, 2024 |
Print ISSN | 0425-1644 |
Electronic ISSN | 2042-3306 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1111/evj.14128 |
Keywords | disease subtypes; EAMS; exercise-associated myopathy syndrome; exertional rhabdomyolysis; myopathy; RER; POLYSACCHARIDE STORAGE MYOPATHY; RECURRENT EXERTIONAL RHABDOMYOLYSIS; ACUTE-RENAL-FAILURE; MYOFIBRILLAR MYOPATHY; THOROUGHBRED RACEHORSES; PERIODIC PA |
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Characterisation Of Phenotypic Patterns In Equine Exercise-associated Myopathies
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Licence
http://creativecommons.org/licenses/by/4.0/
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
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