Fetal growth restriction and placental defects in obese mice are associated with impaired decidualisation: the role of increased leptin signalling modulators SOCS3 and PTPN2

Walewska, E; Makowczenko, KG; Witek, K; Laniecka, E; Molcan, T; Alvarez-Sanchez, A; Kelsey, G; Perez-Garcia, V; Galvao, AM

Abstract

Decidualisation of the endometrium is a key event in early pregnancy, which enables embryo implantation. Importantly, the molecular processes impairing decidualisation in obese mothers are yet to be characterised. We hypothesise that impaired decidualisation in obese mice is mediated by the upregulation of leptin modulators, the suppressor of cytokine signalling 3 (SOCS3) and the protein tyrosine phosphatase non-receptor type 2 (PTPN2), together with the disruption of progesterone (P4)-signal transducer and activator of transcription (STAT3) signalling. After feeding mice with chow diet (CD) or high-fat diet (HFD) for 16 weeks, we confirmed the downregulation of P4 and oestradiol (E2) steroid receptors in decidua from embryonic day (E) 6.5 and decreased proliferation of stromal cells from HFD. In vitro decidualised mouse endometrial stromal cells (MESCs) and E6.5 deciduas from the HFD showed decreased expression of decidualisation markers, followed by the upregulation of SOCS3 and PTPN2 and decreased phosphorylation of STAT3. In vivo and in vitro leptin treatment of mice and MESCs mimicked the results observed in the obese model. The downregulation of Socs3 and Ptpn2 after siRNA transfection of MESCs from HFD mice restored the expression level of decidualisation markers. Finally, DIO mice placentas from E18.5 showed decreased labyrinth development and vascularisation and fetal growth restricted embryos. The present study revealed major defects in decidualisation in obese mice, characterised by altered uterine response to E2 and P4 steroid signalling. Importantly, altered hormonal response was associated with increased expression of leptin signalling modulators SOCS3 and PTPN2. Elevated levels of SOCS3 and PTPN2 were shown to molecularly affect decidualisation in obese mice, potentially disrupting the STAT3-PR regulatory molecular hub.

Citation

Walewska, E., Makowczenko, K., Witek, K., Laniecka, E., Molcan, T., Alvarez-Sanchez, A., Kelsey, G., Perez-Garcia, V., & Galvao, A. (2024). Fetal growth restriction and placental defects in obese mice are associated with impaired decidualisation: the role of increased leptin signalling modulators SOCS3 and PTPN2. Cellular and Molecular Life Sciences, 81(1), https://doi.org/10.1007/s00018-024-05336-7

• Journal Article Type: Article
• Acceptance Date: Jun 28, 2024
• Online Publication Date: Aug 1, 2024
• Publication Date: 2024
• Deposit Date: Dec 3, 2024
• Publicly Available Date: Dec 3, 2024
• Print ISSN: 1420-682X
• Electronic ISSN: 1420-9071
• Publisher: Bioscientifica
• Peer Reviewed: Peer Reviewed
• Volume: 81
• Issue: 1
• DOI: https://doi.org/10.1007/s00018-024-05336-7
• Keywords: Obesity; Endometrium; Decidua; Implantation; Placenta; Leptin; SOCS3; PTPN2; STAT3; DECIDUALIZATION; CELLS; TRANSDUCER; EXPRESSION; ACTIVATOR; ENDOMETRIOSIS; SUPPRESSOR; PREGNANCY; RECEPTOR; PROGRAM