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All Outputs (112)

Escape of TLR5 Recognition by Leptospira spp.: A Rationale for Atypical Endoflagella (2020)
Journal Article
Holzapfel, M., Bonhomme, D., Cagliero, J., Vernel-Pauillac, F., Fanton d’Andon, M., Bortolussi, S., Fiette, L., Goarant, C., Wunder, E. A., Picardeau, M., Ko, A. I., Werling, D., Matsui, M., Boneca, I. G., & Werts, C. (2020). Escape of TLR5 Recognition by Leptospira spp.: A Rationale for Atypical Endoflagella. Frontiers in Immunology, 11, https://doi.org/10.3389/fimmu.2020.02007

Leptospira (L.) interrogans are invasive bacteria responsible for leptospirosis, a worldwide zoonosis. They possess two periplasmic endoflagellae that allow their motility. L. interrogans are stealth pathogens that escape the innate immune recognitio... Read More about Escape of TLR5 Recognition by Leptospira spp.: A Rationale for Atypical Endoflagella.

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis (2020)
Journal Article
Holder, A., Garty, R., Elder, C., Mesnard, P., Laquerbe, C., Bartens, M.-C., Salavati, M., Shabbir, M., Tzelos, T., Connelly, T., Villarreal-Ramos, B., & Werling, D. (2020). Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis. Frontiers in Microbiology, 11, https://doi.org/10.3389/fmicb.2020.01420

Bovine tuberculosis (bTB), caused by Mycobacterium bovis, is a chronic zoonotic disease where host genetics is thought to contribute to susceptibility or resistance. One of the genes implicated is the SLC11A1 gene, that encodes for the natural resist... Read More about Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis.

A Genome-Wide Association Study for Calving Interval in Holstein Dairy Cows Using Weighted Single-Step Genomic BLUP Approach. (2020)
Journal Article
Atashi, H., Salavati, M., De Koster, J., Crowe, M., Opsomer, G., Hostens, M., & Werling, D. (2020). A Genome-Wide Association Study for Calving Interval in Holstein Dairy Cows Using Weighted Single-Step Genomic BLUP Approach. Animals, 10(3),

The aim of the present study was to identify genomic region(s) associated with the length of the calving interval in primiparous (n = 6866) and multiparous (n = 5071) Holstein cows. The single nucleotide polymorphism (SNP) solutions were estimated us... Read More about A Genome-Wide Association Study for Calving Interval in Holstein Dairy Cows Using Weighted Single-Step Genomic BLUP Approach..

Poultry Coccidiosis: Design and Interpretation of Vaccine Studies (2020)
Journal Article
Soutter, F., Werling, D., Tomley, F. M., & Blake, D. P. (2020). Poultry Coccidiosis: Design and Interpretation of Vaccine Studies. Frontiers in Veterinary Science, 7, https://doi.org/10.3389/fvets.2020.00101

Eimeria infection impacts upon chicken welfare and economic productivity of the poultry sector. Live coccidiosis vaccines for chickens have been available for almost 70 years, but the requirement to formulate blends of oocysts from multiple Eimeria s... Read More about Poultry Coccidiosis: Design and Interpretation of Vaccine Studies.

Mammalian lectin arrays for screening host-microbe interactions (2020)
Journal Article
Jégouzo, S. A. F., Nelson, C., Hardwick, T., Wong, S. T. A., Lau, N. K. K., Neoh, G. K. E., Castellanos-Rueda, R., Huang, Z., Mignot, B., Hirdaramani, A., Howitt, A., Frewin, K., Shen, Z., Fox, R. J., Wong, R., Ando, M., Emony, L., Zhu, H., Holder, A. L., Werling, D., …Drickamer, K. (2020). Mammalian lectin arrays for screening host-microbe interactions. Journal of Biological Chemistry, 295(14), 4541-4555. https://doi.org/10.1074/jbc.RA120.012783

Many members of the C-type lectin family of glycan-binding receptors have been ascribed roles in the recognition of microorganisms and serve as key receptors in the innate immune response to pathogens. Other mammalian receptors have become targets th... Read More about Mammalian lectin arrays for screening host-microbe interactions.

Genome-wide association for milk production and lactation curve parameters in Holstein dairy cows. (2019)
Journal Article
Atashi, H., Salavati, M., De Koster, J., Ehrlich, J., Crowe, M., Opsomer, G., Hostens, M., & Werling, D. (2019). Genome-wide association for milk production and lactation curve parameters in Holstein dairy cows. Journal of Animal Breeding and Genetics, 137(3), 292-304. https://doi.org/10.1111/jbg.12442

The aim of this study was to identify genomic regions associated with 305-day milk yield and lactation curve parameters on primiparous (n = 9,910) and multiparous (n = 11,158) Holstein cows. The SNP solutions were estimated using a weighted single-st... Read More about Genome-wide association for milk production and lactation curve parameters in Holstein dairy cows..

CD23 is a glycan-binding receptor in some mammalian species (2019)
Journal Article
Jégouzo, S. A. F., Feinberg, H., Morrison, A., Holder, A., May, A., Huang, Z., Jiang, L., Lasanajak, Y., Smith, D. F., Werling, D., Drickamer, K., Weis, W. I., & Taylor, M. E. (2019). CD23 is a glycan-binding receptor in some mammalian species. Journal of Biological Chemistry, https://doi.org/10.1074/jbc.RA119.010572

CD23, the low affinity IgE receptor found on B lymphocytes and other cells, contains a C-terminal lectin-like domain that resembles C-type carbohydrate-recognition domains (CRDs) found in many glycan-binding receptors. In most mammalian species, the... Read More about CD23 is a glycan-binding receptor in some mammalian species.

Administration of a Synbiotic Containing Enterococcus faecium Does Not Significantly Alter Fecal Microbiota Richness or Diversity in Dogs With and Without Food-Responsive Chronic Enteropathy (2019)
Journal Article
Pilla, R., Guard, B. C., Steiner, J. M., Gaschen, F. P., Olson, E., Werling, D., Allenspach, K., Schmitz, S. S., & Suchodolski, J. S. (2019). Administration of a Synbiotic Containing Enterococcus faecium Does Not Significantly Alter Fecal Microbiota Richness or Diversity in Dogs With and Without Food-Responsive Chronic Enteropathy. Frontiers in Veterinary Science, https://doi.org/10.3389/fvets.2019.00277

Pegbovigrastim treatment in vivo does not affect granulocyte ability to migrate to endometrial cells and kill bacteria in vitro in healthy cows (2019)
Journal Article
Tombacz, K., Peters, L. M., Chang, Y. M., Steele, M., Werling, D., & Gibson, A. J. (2019). Pegbovigrastim treatment in vivo does not affect granulocyte ability to migrate to endometrial cells and kill bacteria in vitro in healthy cows. Journal of Dairy Science, 102(10), 9389-9395. https://doi.org/10.3168/jds.2019-16563

In periparturient dairy cows, immune suppression, resulting in decreased neutrophil numbers and function, leads to increased susceptibility to postpartum conditions such as mastitis, retained placenta, and metritis. Administration of polyethylene gly... Read More about Pegbovigrastim treatment in vivo does not affect granulocyte ability to migrate to endometrial cells and kill bacteria in vitro in healthy cows.

Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence (2019)
Journal Article
García-Sánchez, M., Jiménez-Pelayo, L., Horcajo, P., Regidor-Cerrillo, J., Ólafsson, E. B., Bhandage, A. K., Barragan, A., Werling, D., Ortega-Mora, L. M., & Collantes-Fernández, E. (2019). Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence. Frontiers in Immunology, 10(915), https://doi.org/10.3389/fimmu.2019.00915

Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii, represents one of the main causes of abortion in cattle. Macrophages (MØs) are mediators of the innate immune response against infection and likely one of the first cells en... Read More about Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence.

Potential of milk mid-IR spectra to predict metabolic status of cows through blood components and an innovative clustering approach (2019)
Journal Article
Grelet, C., Vanlierde, A., Hostens, M., Foldager, L., Salavati, M., Ingvartsen, K. L., Crowe, M., Sorensen, M. T., Froidmont, E., Ferris, C. P., Marchitelli, C., Becker, F., Larsen, T., Carter, F., Dehareng, F., McLoughlin, N., Fahey, A., Matthews, E., Santoro, A., Byrne, C., …GplusE Consortium. (2019). Potential of milk mid-IR spectra to predict metabolic status of cows through blood components and an innovative clustering approach. animal, 13(3), 649-658. https://doi.org/10.1017/S1751731118001751

Unbalanced metabolic status in the weeks after calving predisposes dairy cows to metabolic and infectious diseases. Blood glucose, IGF-I, non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB) are used as indicators of the metabolic status of... Read More about Potential of milk mid-IR spectra to predict metabolic status of cows through blood components and an innovative clustering approach.

Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5 (2018)
Journal Article
Tombacz, K., Burgess, G., Holder, A. L., Werners, A., & Werling, D. (2018). Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5. Innate Immunity, https://doi.org/10.1177/1753425918798450

Toxoplasma gondii is responsible for one of the most prevalent infections in people. T. gondii profilin (TgPr) is a protein integral to parasite movement and cellular invasion. Murine TLR has been described to bind TgPr. Furthermore, more recently, h... Read More about Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5.

Genome-wide association studies of inflammatory bowel disease in German shepherd dogs (2018)
Journal Article
Perivan, A., Bertolini, F., Rothschild, M. F., Simpson, K. W., Jergens, A. E., Allenspach, K., & Werling, D. (2018). Genome-wide association studies of inflammatory bowel disease in German shepherd dogs. PLoS ONE, 13(7), e0200685. https://doi.org/10.1371/journal.pone.0200685

Canine Inflammatory Bowel Disease (IBD) is considered a multifactorial disease caused by complex interactions between the intestinal immune system, intestinal microbiota and environmental factors in genetically susceptible individuals. Although IBD c... Read More about Genome-wide association studies of inflammatory bowel disease in German shepherd dogs.

Characterisation of porcine circovirus type 2 in porcine circovirus disease cases in England and Wales (2018)
Journal Article
Grierson, S. S., Werling, D., Bidewell, C., & Williamson, S. (2018). Characterisation of porcine circovirus type 2 in porcine circovirus disease cases in England and Wales. Veterinary Record, 182(1), 22. https://doi.org/10.1136/vr.104450

Confirmed cases of porcine circovirus disease (PCVD) in Great Britain have shown a steady decline since the availability of porcine circovirus type 2 (PCV2) vaccines. However, PCVD is still occasionally diagnosed. The authors carried out a genotyping... Read More about Characterisation of porcine circovirus type 2 in porcine circovirus disease cases in England and Wales.

Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows (2017)
Journal Article
Chen, X., Zhang, S., Cheng, Z. R., Cooke, J. S., Werling, D., Wathes, D. C., & Pollott, G. E. (2017). Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows. PLoS ONE, 12(4), https://doi.org/10.1371/journal.pone.0175555

Selectins are adhesion molecules, which mediate attachment between leucocytes and endothelium. They aid extravasation of leucocytes from blood into inflamed tissue during the mammary gland’s response to infection. Selectins are also involved in attac... Read More about Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows.

Comparison of cellular assays for TLR activation and development of a species-specific reporter cell line for cattle (2017)
Journal Article
Tombacz, K., Mwangi, D., Werling, D., & Gibson, A. J. (2017). Comparison of cellular assays for TLR activation and development of a species-specific reporter cell line for cattle. Innate Immunity, 23(4), 329-335. https://doi.org/10.1177/1753425917695445

PRRs are sentinels of the innate immune system, with TLRs being the most important. Assays for TLR ligand interactions have been used to gain insights into their function and signaling pathways. As significant differences exist between species with r... Read More about Comparison of cellular assays for TLR activation and development of a species-specific reporter cell line for cattle.

Single Nucleotide Polymorphisms in Major histocompatibility class II haplotypes are associated with potential resistance to inflammatory bowel disease in German shepherd dogs (2016)
Journal Article
Peiravan, A., Allenspach, K., Boag, A. M., Soutter, F., Holder, A. L., Catchpole, B., Kennedy, L. J., Werling, D., & Procoli, F. (2016). Single Nucleotide Polymorphisms in Major histocompatibility class II haplotypes are associated with potential resistance to inflammatory bowel disease in German shepherd dogs. Veterinary Immunology and Immunopathology, 182, 101-105. https://doi.org/10.1016/j.vetimm.2016.10.012