Mammalian lectin arrays for screening host-microbe interactions

Jégouzo, S A F; Nelson, C; Hardwick, T; Wong, S T A; Lau, N K K; Neoh, G K E; Castellanos-Rueda, R; Huang, Z; Mignot, B; Hirdaramani, A; Howitt, A; Frewin, K; Shen, Z; Fox, R J; Wong, R; Ando, M; Emony, L; Zhu, H; Holder, A L; Werling, D; Krishnan, N; Robertson, B D; Clements, A; Taylor, M E; Drickamer, K

doi:10.1074/jbc.RA120.012783

Mammalian lectin arrays for screening host-microbe interactions

Jégouzo, S A F; Nelson, C; Hardwick, T; Wong, S T A; Lau, N K K; Neoh, G K E; Castellanos-Rueda, R; Huang, Z; Mignot, B; Hirdaramani, A; Howitt, A; Frewin, K; Shen, Z; Fox, R J; Wong, R; Ando, M; Emony, L; Zhu, H; Holder, A L; Werling, D; Krishnan, N; Robertson, B D; Clements, A; Taylor, M E; Drickamer, K

Authors

S A F Jégouzo

C Nelson

T Hardwick

S T A Wong

N K K Lau

G K E Neoh

R Castellanos-Rueda

Z Huang

B Mignot

A Hirdaramani

A Howitt

K Frewin

Z Shen

R J Fox

R Wong

M Ando

L Emony

H Zhu

A L Holder

D Werling

N Krishnan

B D Robertson

A Clements

M E Taylor

K Drickamer

Abstract

Many members of the C-type lectin family of glycan-binding receptors have been ascribed roles in the recognition of microorganisms and serve as key receptors in the innate immune response to pathogens. Other mammalian receptors have become targets through which pathogens enter target cells. These receptor roles have often been documented with binding studies involving individual pairs of receptors and micro-organisms. To provide a systematic overview of interactions between microbes and the large complement of C-type lectins, here we developed a lectin array and suitable protocols for labeling of microbes that could be used to probe this array. The array contains C-type lectins from cow, chosen as a model organism of agricultural interest for which the relevant pathogen–receptor interactions have not been previously investigated in detail. Screening with yeast cells and various strains of both Gram-positive and -negative bacteria revealed distinct binding patterns, which in some cases could be explained by binding to lipopolysaccharides or capsular polysaccharides, but in other cases suggested the presence of novel glycan targets on many of the microorganisms. These results are consistent with interactions previously ascribed to the receptors, but also highlight binding to additional sugar targets that have not previously been recognized. Our findings indicate that mammalian lectin arrays represent unique discovery tools for identifying both novel ligands and new receptor functions.

Citation

Jégouzo, S. A. F., Nelson, C., Hardwick, T., Wong, S. T. A., Lau, N. K. K., Neoh, G. K. E., Castellanos-Rueda, R., Huang, Z., Mignot, B., Hirdaramani, A., Howitt, A., Frewin, K., Shen, Z., Fox, R. J., Wong, R., Ando, M., Emony, L., Zhu, H., Holder, A. L., Werling, D., …Drickamer, K. (2020). Mammalian lectin arrays for screening host-microbe interactions. Journal of Biological Chemistry, 295(14), 4541-4555. https://doi.org/10.1074/jbc.RA120.012783

Journal Article Type	Article
Acceptance Date	Feb 24, 2020
Publication Date	Feb 24, 2020
Deposit Date	Mar 11, 2020
Publicly Available Date	Mar 11, 2020
Journal	JOURNAL OF BIOLOGICAL CHEMISTRY
Print ISSN	0021-9258
Electronic ISSN	1083-351X
Publisher	American Society for Biochemistry and Molecular Biology
Peer Reviewed	Peer Reviewed
Volume	295
Issue	14
Pages	4541-4555
DOI	https://doi.org/10.1074/jbc.RA120.012783
Public URL	https://rvc-repository.worktribe.com/output/1378516