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Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5

Tombacz, K; Burgess, G; Holder, A L; Werners, A; Werling, D


K Tombacz

G Burgess

A L Holder

A Werners

D Werling


Toxoplasma gondii is responsible for one of the most prevalent infections in people. T. gondii profilin (TgPr) is a protein integral to parasite movement and cellular invasion. Murine TLR has been described to bind TgPr. Furthermore, more recently, human TLR5 has been described to recognise recombinant TgPr, as well as bacterial flagellin. In addition to infections in humans, T. gondii infects farm animals, but little information is available about its innate recognition. We aimed to investigate whether, similarly to their human orthologue, bovine and porcine TLR5 could also be stimulated by TgPr by using a combination of reporter cell lines expressing full length TLR5 from each species as well as primary cells. Although human and bovine TLR5-transfected cells responded to flagellin, no response was detected upon stimulation
with profilin. Furthermore, TgPr failed to elicit IL-6 secretion in human peripheral blood mononuclear cells and CD14þ monocytes. In contrast, exposure of RAW cells, known to express TLR11 to TgPr, slightly increased the IL-6 response. Our data cast doubts on the possibility that profilin is a specific ligand for human TLR5 and bovine TLR5. This leaves the immunogenic properties of this potential target antigen uncharacterised outside of the murine system.


Tombacz, K., Burgess, G., Holder, A. L., Werners, A., & Werling, D. (2018). Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5. Innate Immunity,

Journal Article Type Article
Acceptance Date Aug 9, 2018
Publication Date Sep 8, 2018
Deposit Date Aug 29, 2018
Publicly Available Date Dec 11, 2020
Journal Innate Immunity
Print ISSN 1753-4259
Publisher SAGE Publications
Peer Reviewed Peer Reviewed
Public URL


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