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Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do (2020)
Journal Article
Ribitsch, I., Baptista, P. M., Lange-Consiglio, A., Melotti, L., Patruno, M., Jenner, F., …Penning, L. C. (2020). Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do. Frontiers in Bioengineering and Biotechnology, 8,

Rapid developments in Regenerative Medicine and Tissue Engineering has witnessed an increasing drive toward clinical translation of breakthrough technologies. However, the progression of promising preclinical data to achieve successful clinical marke... Read More about Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do.

Heterogeneity of proteome dynamics between connective tissue phases of adult tendon (2020)
Journal Article
Choi, H., Simpson, D., Wang, D., Prescott, M., Pitsillides, A. A., Dudhia, J., …Thorpe, C. T. (2020). Heterogeneity of proteome dynamics between connective tissue phases of adult tendon. eLife, 9, https://doi.org/10.7554/elife.55262

Maintenance of connective tissue integrity is fundamental to sustain function, requiring protein turnover to repair damaged tissue. However, connective tissue proteome dynamics remain largely undefined, as do differences in turnover rates of individu... Read More about Heterogeneity of proteome dynamics between connective tissue phases of adult tendon.

Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease (2020)
Journal Article
Smith, R. K. W., Önnerfjord, P., Holmgren, K., di Grado, S., & Dudhia, J. (2020). Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease. International Journal of Molecular Sciences, 21(6), 2155. https://doi.org/10.3390/ijms21062155

The diagnosis of tendon injury relies on clinical signs and diagnostic imaging but imaging is subjective and does not always correlate with clinical signs. A molecular marker would potentially offer a sensitive and specific diagnostic tool that could... Read More about Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease.

Histopathological and immunohistochemical evaluation of cellular response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch for tendon repair (2020)
Journal Article
Rashid, M., Dudhia, J., Dakin, S. G., Snelling, S. J. B., De Godoy, R., Mouthuy, P., …Carr, A. J. (2020). Histopathological and immunohistochemical evaluation of cellular response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch for tendon repair. Scientific Reports, 10(1), https://doi.org/10.1038/s41598-020-61725-5

We investigated endogenous tissue response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch intended for tendon repair. A sheep tendon injury model characterised by a natural history of consistent failure of healing was... Read More about Histopathological and immunohistochemical evaluation of cellular response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch for tendon repair.

Inducing pluripotency in the domestic cat (Felis Catus) (2019)
Journal Article
Dutton, L. C., Dudhia, J., Guest, D. J., & Connolly, D. J. (2019). Inducing pluripotency in the domestic cat (Felis Catus). Stem Cells and Development, 28(19), 1299-1309. https://doi.org/10.1089/scd.2019.0142

Domestic cats suffer from a range of inherited genetic diseases, many of which display similarities with equivalent human conditions. Developing cellular models for these inherited diseases would enable drug discovery, benefiting feline health and we... Read More about Inducing pluripotency in the domestic cat (Felis Catus).

Evaluation of the Effects of Synovial Multipotent Cells on Deep Digital Flexor Tendon Repair in a Large Animal Model of Intra-Synovial Tendinopathy (2019)
Journal Article
Khan, M. R., Smith, R. K., David, F. H., Lam, R., Hughes, G., DeGodoy, R., …Dudhia, J. (2019). Evaluation of the Effects of Synovial Multipotent Cells on Deep Digital Flexor Tendon Repair in a Large Animal Model of Intra-Synovial Tendinopathy. Journal of Orthopaedic Research, 38(1), 128-138. https://doi.org/10.1002/jor.24423

ntra‐synovial tendon injuries are a common orthopedic problem with limited treatment options. The synovium is a specialized connective tissue forming the inner encapsulating lining of diarthrodial joints and intra‐synovial tendons. It contains multip... Read More about Evaluation of the Effects of Synovial Multipotent Cells on Deep Digital Flexor Tendon Repair in a Large Animal Model of Intra-Synovial Tendinopathy.

Bone marrow mesenchymal stem cells do not enhance intra-synovial tendon healing despite engraftment and homing to niches within the synovium (2018)
Journal Article
Khan, M. R., Dudhia, J., David, F. H., De Godoy, R., Mehra, V., Hughes, G., …Smith, R. K. W. (2018). Bone marrow mesenchymal stem cells do not enhance intra-synovial tendon healing despite engraftment and homing to niches within the synovium. https://doi.org/10.1186/s13287-018-0900-7

Intra-synovial tendon injuries display poor healing, which often results in reduced functionality and pain. A lack of effective therapeutic options has led to experimental approaches to augment natural tendon repair with autologous mesenchymal stem c... Read More about Bone marrow mesenchymal stem cells do not enhance intra-synovial tendon healing despite engraftment and homing to niches within the synovium.

Exposure of a tendon extracellular matrix to synovial fluid triggers endogenous and engrafted cell death: A mechanism for failed healing of intrathecal tendon injuries (2016)
Journal Article
Garvican, E. R., Salavati, M., Smith, R. K. W., & Dudhia, J. (2016). Exposure of a tendon extracellular matrix to synovial fluid triggers endogenous and engrafted cell death: A mechanism for failed healing of intrathecal tendon injuries. Connective Tissue Research, 58(5), 438-446. https://doi.org/10.1080/03008207.2016.1245726

Aim: The purpose of this study was to investigate the effect of normal synovial fluid (SF) on exposed endogenous tendon-derived cells (TDC) and engrafted mesenchymal stem cells (MSCs) within the tendon extracellular matrix. Methods: Explants from eq... Read More about Exposure of a tendon extracellular matrix to synovial fluid triggers endogenous and engrafted cell death: A mechanism for failed healing of intrathecal tendon injuries.

Investigating the Postmortem Molecular Biology of Cartilage and its Potential Forensic Applications (2015)
Journal Article
Bolton, S. N., Whitehead, M. P., Dudhia, J., Baldwin, T. C., & Sutton, R. (2015). Investigating the Postmortem Molecular Biology of Cartilage and its Potential Forensic Applications. Journal of Forensic Sciences, 60(4), 1061-67. https://doi.org/10.1111/1556-4029.12764

This study investigated the postmortem molecular changes that articular cartilage undergoes following burial. Fresh pig trotters were interred in 30‐cm‐deep graves at two distinct locations exhibiting dissimilar soil environments for up to 42 days. E... Read More about Investigating the Postmortem Molecular Biology of Cartilage and its Potential Forensic Applications.

Low Density Lipoprotein Receptor-related Protein 1 (LRP1)-mediated Endocytic Clearance of a Disintegrin and Metalloproteinase with Thrombospondin Motifs-4 (ADAMTS-4) FUNCTIONAL DIFFERENCES OF NON-CATALYTIC DOMAINS OF ADAMTS-4 AND ADAMTS-5 IN LRP1 BINDING (2014)
Journal Article
Yamamoto, K., Owen, K., Parker, A. E., Scilabra, S. D., Dudhia, J., Strickland, D. K., …Nagase, H. (2014). Low Density Lipoprotein Receptor-related Protein 1 (LRP1)-mediated Endocytic Clearance of a Disintegrin and Metalloproteinase with Thrombospondin Motifs-4 (ADAMTS-4) FUNCTIONAL DIFFERENCES OF NON-CATALYTIC DOMAINS OF ADAMTS-4 AND ADAMTS-5 IN LRP1 BINDING. Journal of Biological Chemistry, 289(10), 6462-6474. https://doi.org/10.1074/jbc.M113.545376

Degradation of the cartilage proteoglycan aggrecan is an early event in the development of osteoarthritis, and a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4) and ADAMTS-5 are considered to be the major aggrecan-degrading... Read More about Low Density Lipoprotein Receptor-related Protein 1 (LRP1)-mediated Endocytic Clearance of a Disintegrin and Metalloproteinase with Thrombospondin Motifs-4 (ADAMTS-4) FUNCTIONAL DIFFERENCES OF NON-CATALYTIC DOMAINS OF ADAMTS-4 AND ADAMTS-5 IN LRP1 BINDING.

Proteomic Analysis of Tendon Extracellular Matrix Reveals Disease Stage- specific Fragmentation and Differential Cleavage of COMP ( Cartilage Oligomeric Matrix Protein) (2014)
Journal Article
Dakin, S. G., Smith, R. K. W., Heinegard, D., Onnerfjord, P., Khabut, A., & Dudhia, J. (2014). Proteomic Analysis of Tendon Extracellular Matrix Reveals Disease Stage- specific Fragmentation and Differential Cleavage of COMP ( Cartilage Oligomeric Matrix Protein). Journal of Biological Chemistry, 289(8), 4919-4927. https://doi.org/10.1074/jbc.M113.511972

During inflammatory processes the extracellular matrix (ECM) is extensively remodeled, and many of the constituent components are released as proteolytically cleaved fragments. These degradative processes are better documented for inflammatory joint... Read More about Proteomic Analysis of Tendon Extracellular Matrix Reveals Disease Stage- specific Fragmentation and Differential Cleavage of COMP ( Cartilage Oligomeric Matrix Protein).

A preliminary study on the effect of wounding on transforming growth factor-ß1 and cartilage oligomeric matrix protein concentrations in the skin of horses (2011)
Journal Article
Dart, A. J., Dart, C. M., Dudhia, J., Perkins, N., Canfield, P. J., & Smith, R. K. W. (2011). A preliminary study on the effect of wounding on transforming growth factor-ß1 and cartilage oligomeric matrix protein concentrations in the skin of horses. Veterinary Surgery, 40(1), 59-65. https://doi.org/10.1111/j.1532-950X.2010.00756.x

Equine Embryonic Stem Cell-Derived Tenocytes are Insensitive to a Combination of Inflammatory Cytokines and Have Distinct Molecular Responses Compared to Primary Tenocytes (0000)
Journal Article
Smith, E. J., Beaumont, R. E., Dudhia, J., & Guest, D. J. (0000). Equine Embryonic Stem Cell-Derived Tenocytes are Insensitive to a Combination of Inflammatory Cytokines and Have Distinct Molecular Responses Compared to Primary Tenocytes. Stem Cell Reviews and Reports, https://doi.org/10.1007/s12015-024-10693-8

Tissue fibrosis following tendon injury is a major clinical problem due to the increased risk of re-injury and limited treatment options; however, its mechanism remains unclear. Evidence suggests that insufficient resolution of inflammation contribut... Read More about Equine Embryonic Stem Cell-Derived Tenocytes are Insensitive to a Combination of Inflammatory Cytokines and Have Distinct Molecular Responses Compared to Primary Tenocytes.