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Isolation and generation of osteoblasts

Perpetuo, I P; Bourne, L E; Orriss, I R


I P Perpetuo

L E Bourne

I R Orriss


This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.


Perpetuo, I. P., Bourne, L. E., & Orriss, I. R. (2019). Isolation and generation of osteoblasts. In Bone Research Protocols. New York: Humana Press.

Publication Date Feb 8, 2019
Deposit Date Feb 16, 2019
Book Title Bone Research Protocols
ISBN 978-1-4939-8997-3
Public URL