Skip to main content

Research Repository

Advanced Search

Association between Single Nucleotide Polymorphism in RelA with Somatic Cell Count and Longevity Supports Importance of NF-¦ÊB Signalling in Cattle Health

Chen, X; Zhang, S; Cheng, Z R; Pollott, G E; Wathes, D C

Authors

X Chen

S Zhang

Z R Cheng

G E Pollott

D C Wathes



Abstract

Mastitis reduces milk production and causes culling. The NF-κB transcription factor RelA plays a central regulatory role in innate immunity. This study used a candidate gene approach to investigate associations between the synonymous C/G SNP rs48035703 in RELA with somatic cell count (SCC) and survival time. Blood samples were collected from 337 Holstein-Friesian heifers on 19 farms and genotyped by PCR-restriction fragment length polymorphism. Animals were monitored from 6 months until 2340 d of age. Pedigree, milk production and disease records were obtained. Genotype frequencies were CC 0.63, CG 0.30 and GG 0.06. The C allele had a favourable additive effect on survival: average longevities from birth were CC, 1872 d; CG, 1745 d and GG 1596 d (P < 0.003). Log transformed first lactation somatic cell count (SCC)data showed a significant association with this SNP using an allele substitution model (mean residuals ± SD: GG 0.30 ± 1.263; CG 0.22 ± 0.994, CC −0.04 ± 0.803, P < 0.05). More CC cows than expected were classified as intermediate and fewer as mastitic (30.4% v 45.9%) with respect to SCC class when categorised as 0 (unaffected), 1 (intermediate) and 2 (mastitic), whereas for CG heterozygotes fewer were intermediate and more were mastitic (12.1% v 60.3%) (p = 0.05). RELA rs48035703 CC genotype cows were therefore less likely to experience a high SCC and survived longer. These results support a role for RelA in combating mammary gland infection and warrant further studies in additional populations.

Citation

Chen, X., Zhang, S., Cheng, Z. R., Pollott, G. E., & Wathes, D. C. (2019). Association between Single Nucleotide Polymorphism in RelA with Somatic Cell Count and Longevity Supports Importance of NF-¦ÊB Signalling in Cattle Health. Open Journal of Animal Sciences, 9(1), 51-64. https://doi.org/10.4236/ojas.2019.91005

Journal Article Type Article
Acceptance Date Jan 1, 2019
Publication Date Jan 4, 2019
Deposit Date Jan 29, 2019
Publicly Available Date Mar 28, 2024
Journal Open Journal of Animal Sciences
Print ISSN 2161-7597
Publisher Scientific Research Publishing
Peer Reviewed Peer Reviewed
Volume 9
Issue 1
Pages 51-64
DOI https://doi.org/10.4236/ojas.2019.91005
Public URL https://rvc-repository.worktribe.com/output/1384108

Files




You might also like



Downloadable Citations