Dynamic changes in gene expression and signalling during trophoblast development in the horse

Read, J E; Cabrera-Sharp, V; Offord, V; Mirczuk, S M; Allen, S P; Fowkes, R C; De Mestre, A M

Abstract

Equine chorionic girdle trophoblast cells play important endocrine and immune functions critical in supporting pregnancy. Very little is known about the genes and pathways that regulate chorionic girdle trophoblast development. Our aim was to identify genes and signalling pathways active in vivo in equine chorionic girdle trophoblast within a critical 7 days window. We exploited the late implantation of the equine conceptus to obtain trophoblast tissue. An Agilent equine 44K microarray was performed using RNA extracted from Chorionic Girdle and Chorion (control) from equine pregnancy days 27, 30, 31 and 34 (n=5), corresponding to the initiation of chorionic girdle trophoblast proliferation, differentiation and migration. Data was analysed using R packages limma and maSigPro, Ingenuity Pathway Analysis and DAVID and verified using qRT-PCR, promoter analysis, western blotting and migration assays. Microarray analysis showed gene expression (absolute log FC > 2, FDR-adjusted P

Citation

Read, J. E., Cabrera-Sharp, V., Offord, V., Mirczuk, S. M., Allen, S. P., Fowkes, R. C., & De Mestre, A. M. (2018). Dynamic changes in gene expression and signalling during trophoblast development in the horse. Reproduction, 156(4), 313-330. https://doi.org/10.1530/REP-18-0270

• Journal Article Type: Article
• Acceptance Date: Jul 10, 2018
• Publication Date: Jul 11, 2018
• Deposit Date: Jul 12, 2018
• Publicly Available Date: Nov 8, 2018
• Journal: REPRODUCTION
• Print ISSN: 1470-1626
• Publisher: Society for Reproduction and Fertility
• Peer Reviewed: Peer Reviewed
• Volume: 156
• Issue: 4
• Pages: 313-330
• DOI: https://doi.org/10.1530/REP-18-0270
• Public URL: https://rvc-repository.worktribe.com/output/1386611