Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells

Szczepanski, A; Owczarek, K; Milewska, A; Baster, Z; Rajfur, Z; Mitchell, J A; Pyrc, K

Abstract

Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization.

Citation

Szczepanski, A., Owczarek, K., Milewska, A., Baster, Z., Rajfur, Z., Mitchell, J. A., & Pyrc, K. (2018). Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells. Veterinary Research, 49, 55. https://doi.org/10.1186/s13567-018-0551-9

• Journal Article Type: Article
• Acceptance Date: Jun 14, 2018
• Publication Date: Jul 3, 2018
• Deposit Date: Jul 28, 2018
• Publicly Available Date: Jul 30, 2018
• Journal: VETERINARY RESEARCH
• Print ISSN: 0928-4249
• Publisher: BioMed Central
• Peer Reviewed: Peer Reviewed
• Volume: 49
• Pages: 55
• DOI: https://doi.org/10.1186/s13567-018-0551-9
• Public URL: https://rvc-repository.worktribe.com/output/1386680