A Goodarzi
Effect of melatonin supplementation in the long-term preservation of the sheep ovaries at different temperatures and subsequent in vitro embryo production
Goodarzi, A; Zare Shahneh, A; Kohram, H; Sadeghi, M; Moazenizadeh, M H; Fouladi-Nashta, A A; Dadashpour Davachi, N
Authors
A Zare Shahneh
H Kohram
M Sadeghi
M H Moazenizadeh
A A Fouladi-Nashta
N Dadashpour Davachi
Abstract
Investigations in the past decades have shown that oocytes developmental competence following in vitro fertilization is greatly influenced by an interval between isolation of the ovaries immediately after death/slaughter and oocytes recovery from the visible follicles. In order to determine the optimal conditions for long-term preservation of ovaries, an experiment was conducted with adding different doses of melatonin (0 (C), 500 (M1), 600 (M2), 700 (M3) and 800 (M4) μM) as an antioxidant to sheep ovaries preservation medium (PBS) maintained at 4 and 20 °C for 24 h. The effects on in vitro embryo production (IVEP) parameters including maturation, fertilization, cleavage, and blastocyst rates and the total number of blastomere were evaluated after the ovaries preservation. Melatonin reduced the decline in fertilization rate as an indicator of success in vitro maturation (P ≤ 0.05). Furthermore, ovarian storage time had significant negative effect (P ≤ 0.05) on IVEP parameters. Supplementation with melatonin increased the total cell number of blastocysts as an indicator of embryo quality (i.e. mean blastomeric cells in 4Math Eq groups: 86.00 ± 3.00, 98.50 ± 3.5, 111.5 ± 1.5, 125.5 ± 2.00 and 126.50 ± 5.5 for C, M1, M2, M3 and M4. respectively). Overall, the results showed that the use of melatonin antioxidant in the ovaries storage medium had beneficial effects on sheep oocytes development and embryos quality.
Citation
Goodarzi, A., Zare Shahneh, A., Kohram, H., Sadeghi, M., Moazenizadeh, M. H., Fouladi-Nashta, A. A., & Dadashpour Davachi, N. (2018). Effect of melatonin supplementation in the long-term preservation of the sheep ovaries at different temperatures and subsequent in vitro embryo production. Theriogenology, 106, 265-270. https://doi.org/10.1016/j.theriogenology.2017.10.009
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 7, 2017 |
Publication Date | Jan 15, 2018 |
Deposit Date | Dec 15, 2017 |
Publicly Available Date | Jan 15, 2019 |
Journal | THERIOGENOLOGY |
Print ISSN | 0093-691X |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 106 |
Pages | 265-270 |
DOI | https://doi.org/10.1016/j.theriogenology.2017.10.009 |
Public URL | https://rvc-repository.worktribe.com/output/1389221 |
Files
11064.pdf
(1.5 Mb)
PDF
You might also like
Initiation of a conserved trophectoderm program in human, cow and mouse embryos
(2020)
Journal Article
Polyunsaturated fatty acids influence offspring sex ratio in cows
(2019)
Journal Article
Effect of oral administration of pioglitazone on follicular dynamics in Holstein dairy cows
(2019)
Journal Article
Downloadable Citations
About RVC Repository
Administrator e-mail: publicationsrepos@rvc.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search