Effect of melatonin supplementation in the long-term preservation of the sheep ovaries at different temperatures and subsequent in vitro embryo production

Goodarzi, A; Zare Shahneh, A; Kohram, H; Sadeghi, M; Moazenizadeh, M H; Fouladi-Nashta, A A; Dadashpour Davachi, N

Abstract

Investigations in the past decades have shown that oocytes developmental competence following in vitro fertilization is greatly influenced by an interval between isolation of the ovaries immediately after death/slaughter and oocytes recovery from the visible follicles. In order to determine the optimal conditions for long-term preservation of ovaries, an experiment was conducted with adding different doses of melatonin (0 (C), 500 (M1), 600 (M2), 700 (M3) and 800 (M4) μM) as an antioxidant to sheep ovaries preservation medium (PBS) maintained at 4 and 20 °C for 24 h. The effects on in vitro embryo production (IVEP) parameters including maturation, fertilization, cleavage, and blastocyst rates and the total number of blastomere were evaluated after the ovaries preservation. Melatonin reduced the decline in fertilization rate as an indicator of success in vitro maturation (P ≤ 0.05). Furthermore, ovarian storage time had significant negative effect (P ≤ 0.05) on IVEP parameters. Supplementation with melatonin increased the total cell number of blastocysts as an indicator of embryo quality (i.e. mean blastomeric cells in 4Math Eq groups: 86.00 ± 3.00, 98.50 ± 3.5, 111.5 ± 1.5, 125.5 ± 2.00 and 126.50 ± 5.5 for C, M1, M2, M3 and M4. respectively). Overall, the results showed that the use of melatonin antioxidant in the ovaries storage medium had beneficial effects on sheep oocytes development and embryos quality.

Citation

Goodarzi, A., Zare Shahneh, A., Kohram, H., Sadeghi, M., Moazenizadeh, M. H., Fouladi-Nashta, A. A., & Dadashpour Davachi, N. (2018). Effect of melatonin supplementation in the long-term preservation of the sheep ovaries at different temperatures and subsequent in vitro embryo production. Theriogenology, 106, 265-270. https://doi.org/10.1016/j.theriogenology.2017.10.009

• Journal Article Type: Article
• Acceptance Date: Oct 7, 2017
• Publication Date: Jan 15, 2018
• Deposit Date: Dec 15, 2017
• Publicly Available Date: Jan 15, 2019
• Journal: THERIOGENOLOGY
• Print ISSN: 0093-691X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 106
• Pages: 265-270
• DOI: https://doi.org/10.1016/j.theriogenology.2017.10.009
• Public URL: https://rvc-repository.worktribe.com/output/1389221