The effects of intrauterine infusion of peanut oil on endometrial health, salivary cortisol and interovulatory period in mares

Campbell, M L H; Hampshire, D; Hamstead, L; Rose, B; Smith, K C; De Mestre, A M

Abstract

Intrauterine infusion of peanut oil at Day 10 post-ovulation has been reported to prolong dioestrus in mares. However, the effects of peanut oil treatment on the endometrium and whether the technique is painful have not been assessed. The objectives of this study were, (i) to determine the effect of intrauterine infusion of peanut oil on endometrial health, (ii) to determine whether use of intrauterine peanut oil is painful and (iii) to confirm that peanut oil causes prolonged dioestrus. Six mares aged 3–12 years old were used in a cross-over design with each mare administered both 1 ml of intrauterine peanut oil and a sham treatment on different oestrous cycles. The effect of intrauterine infusion of 1 ml peanut oil or sham treatment were measured using interovulatory period, uterine fluid accumulation as determined by transrectal ultrasonography, serum progesterone levels, endometrial Kenney biopsy scores and histological features, endometrial eosinophil numbers and salivary cortisol measurements. The individual mare response to intrauterine infusion of peanut oil was variable. Peanut oil infusion did not statistically prolong the luteal phase, nor elevate salivary cortisol levels but did cause superficial erosion of the endometrial surface epithelium in all mares and significantly increased eosinophil numbers in the endometrium (P = 0.0068). The Kenney grade for biopsies from 2/6 mares worsened transiently following infusion. In conclusion, intra-uterine peanut oil does not statistically increase the duration of the luteal phase but results in an inflammatory response and increase in endometrial eosinophil numbers suggesting treatment may be associated with a hypersensitivity-type reaction. Those contemplating using peanut oil to suppress oestrus should also be aware of the legislative and regulatory implications.

Citation

Campbell, M. L. H., Hampshire, D., Hamstead, L., Rose, B., Smith, K. C., & De Mestre, A. M. (2017). The effects of intrauterine infusion of peanut oil on endometrial health, salivary cortisol and interovulatory period in mares. Theriogenology, 102, 116-125. https://doi.org/10.1016/j.theriogenology.2017.07.009

• Journal Article Type: Article
• Acceptance Date: Jul 10, 2017
• Publication Date: Jul 13, 2017
• Deposit Date: Jul 18, 2017
• Publicly Available Date: Jul 14, 2018
• Journal: THERIOGENOLOGY
• Print ISSN: 0093-691X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 102
• Pages: 116-125
• DOI: https://doi.org/10.1016/j.theriogenology.2017.07.009
• Public URL: https://rvc-repository.worktribe.com/output/1391248