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Bovine Endometrial Cells Mount Innate Immune Response to the Intracellular Ligands CL097 and Poly(dA:dT) Indicating Roles against Uterine Viruses

Oguejiofor, C F; Cheng, Z R; Fouladi-Nashta, A A; Wathes, D C

Authors

C F Oguejiofor

Z R Cheng

A A Fouladi-Nashta

D C Wathes



Abstract

Uterine infection and endometritis cause infertility and economic losses in the cattle industry. The innate immune response of the endometrium is critical in the elimination of pathogenic organisms that invade the uterus in postpartum cows. This study investigated the response of bovine endometrium to synthetic intracellular ligands which activate innate immunity by stimulating similar receptors to those used to recognise the presence of some viruses. Mixed primary epithelial and stromal cell cultures were treated with 5 μg/ml of CL097 (a TLR7/8 ligand) or 2 μg/ml of poly(dA:dT) (a DNA analogue) for either 6 h or 24 h. Cellular responses were assessed by the mRNA expression of 18 immune-related genes and 3 endogenous reference genes by conventional PCR followed by qRT-PCR from four replicate experiments. Bovine endometrial cells expressed the cytosolic pattern recognition receptors (PRRs) DDX58 (RIG-I), IFIH1 (MDA5) and LRRFIP1 which act as intracellular nucleic acid sensors. Neither ligand altered the expression of the extra-cytosolic pattern recognition receptors (PRRs) TLR3, TLR4, TLR7 or TLR8 whereas poly(dA:dT) treatment increased the expression of IFIH1 and DDX58. Treated cells also responded to CL097 or poly(dA:dT) with a differential up-regulation of genes involved in innate immune response including type I interferon/antiviral response (MX1, IFNAR1), antimicrobial activity (MUC1, SLPI) and cytokine activity (TNF, IL1B, IL8). Bovine endometrial cells therefore express both cytosolic and extra-cytosolic intracellular PRRs and are able to mount an innate immune response upon stimulation with intracellular ligands. This suggests an important role for these cells in the defence against viruses that may be present in the uterus in postpartum cows.

Citation

Oguejiofor, C. F., Cheng, Z. R., Fouladi-Nashta, A. A., & Wathes, D. C. (2017). Bovine Endometrial Cells Mount Innate Immune Response to the Intracellular Ligands CL097 and Poly(dA:dT) Indicating Roles against Uterine Viruses. Open Journal of Animal Sciences, 7(2), https://doi.org/10.4236/ojas.2017.72010

Journal Article Type Article
Acceptance Date Apr 14, 2017
Publication Date Apr 17, 2017
Deposit Date Apr 21, 2017
Publicly Available Date Mar 29, 2024
Journal Open Journal of Animal Sciences
Print ISSN 2161-7597
Publisher Scientific Research Publishing
Peer Reviewed Peer Reviewed
Volume 7
Issue 2
DOI https://doi.org/10.4236/ojas.2017.72010
Public URL https://rvc-repository.worktribe.com/output/1392289

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