Leukocyte Adhesion Under Hemodynamic Flow Conditions

Lawson, C; Rose, M; Wolf, S

Abstract

Vascular endothelial cells (ECs) line the luminal side of all blood vessels and act as a selective barrier between blood and tissue. ECs are constantly exposed to biochemical and biomechanical stimuli from the blood and underlying tissue. Fluid shear stress acts in parallel to the vessel wall, resulting from friction of blood against EC. Despite the importance of flow on normal EC function, much of the information regarding EC function and dysfunction has been derived from cells harvested, grown, and studied in static culture. In order to study the effects of shear stress on EC function a number of in vitro models have been developed. This manuscript provides methodology for use of a system which enables recirculation of leukocytes and cell culture medium over the endothelium for a period of several minutes to days and enables investigation of the effects of prolonged leukocyte coculture on both the endothelial and leukocyte populations.

Citation

Lawson, C., Rose, M., & Wolf, S. (2017). Leukocyte Adhesion Under Hemodynamic Flow Conditions. https://doi.org/10.1007/978-1-4939-6931-9_7

• Journal Article Type: Article
• Publication Date: Mar 28, 2017
• Deposit Date: Jun 28, 2018
• Journal: Methods in Molecular Biology
• Pages: 85-100
• Book Title: T-Cell Trafficking: Methods and Protocols
• ISBN: 978-1-4939-6931-9
• DOI: https://doi.org/10.1007/978-1-4939-6931-9_7
• Public URL: https://rvc-repository.worktribe.com/output/1392586