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Leukocyte Adhesion Under Hemodynamic Flow Conditions

Lawson, C; Rose, M; Wolf, S


C Lawson

M Rose

S Wolf


Vascular endothelial cells (ECs) line the luminal side of all blood vessels and act as a selective barrier between blood and tissue. ECs are constantly exposed to biochemical and biomechanical stimuli from the blood and underlying tissue. Fluid shear stress acts in parallel to the vessel wall, resulting from friction of blood against EC. Despite the importance of flow on normal EC function, much of the information regarding EC function and dysfunction has been derived from cells harvested, grown, and studied in static culture. In order to study the effects of shear stress on EC function a number of in vitro models have been developed. This manuscript provides methodology for use of a system which enables recirculation of leukocytes and cell culture medium over the endothelium for a period of several minutes to days and enables investigation of the effects of prolonged leukocyte coculture on both the endothelial and leukocyte populations.


Lawson, C., Rose, M., & Wolf, S. (2017). Leukocyte Adhesion Under Hemodynamic Flow Conditions.

Journal Article Type Article
Publication Date Mar 28, 2017
Deposit Date Jun 28, 2018
Journal Methods in Molecular Biology
Pages 85-100
Book Title T-Cell Trafficking: Methods and Protocols
ISBN 978-1-4939-6931-9
Public URL

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